BIOD 171 LAB 8 Exam
1. The word ‘antigen’ is actually a combination for what two words?
Antibody-generating
2. For the ELISA assay depicted below, what do the arrows indicate?
The arrows indicate the wash steps.
3. What would happen in terms of the colorimetric readout if you forgot
to do the wash step afterStep D (above)?
All of the wells would have a dark color. The intensity of the readout
is dependent upon the amount of the labeled secondary antibody
present in the well. Since the unbound secondaryantibody was not
washed away, all wells would react equally.
1. In a Western blot are separated based on its size.
proteins
2. When setting up a western blot, what is the purpose of the blocking step
post-transfer?
Blocking prevents non-specific binding of the antibody—you
1. The word ‘antigen’ is actually a combination for what two words?
Antibody-generating
2. For the ELISA assay depicted below, what do the arrows indicate?
The arrows indicate the wash steps.
3. What would happen in terms of the colorimetric readout if you forgot
to do the wash step afterStep D (above)?
All of the wells would have a dark color. The intensity of the readout
is dependent upon the amount of the labeled secondary antibody
present in the well. Since the unbound secondaryantibody was not
washed away, all wells would react equally.
1. In a Western blot are separated based on its size.
proteins
2. When setting up a western blot, what is the purpose of the blocking step
post-transfer?
Blocking prevents non-specific binding of the antibody—you
