Unit 15 Microbiological Techniques
Assignment No: 2 Cultiatng Micro-Organisms
Using Aseptic techniques to cultiivte Micro-Orgvnisms
AIM of the Experiment:
To show a method of the lawn plate to show cell culture techniques.
To show and describe methods using aseptic techniques to safely carry out a practical
investigation.
To explain the applications of cell cultures in research and possible development.
To explain & show how valuable aseptic techniques are to this area.
Introduction:
The term "micro-organism" or microbe, refers to any single-celled living organism, including
bacteria, viruses and fungi. Microbes ate transferred by direct contact or indirectly through a
vehicle (e.g. a surgical tool) or via the air a person breathes. If favourable conditions for
growth exist a new host, microbes can establish themselves and reproduce colonies.
Many types of organisms are pathogenic, (foreign body) and when they enter the body, they
cause infection. Here they can damage the cells directly or release toxins that will eventually
cause damage. The prevention of disease-causing microbes especially in hospitals for
example or patient care, is generally accomplished using "Aseptic or sterile techniques".
Here is then the goal is to create a germ free environment as much as is deemed possible.
This is achieved through aseptic techniques and sterilization and the maintenance of
sterile/non-sterile barriers.
In this particular experiment we are showing and explaining the principles underlying the
microbiological techniques used in terms of relevant aseptic techniques and the method of
cultivation.
Within this report of the practical carried out there will be an investigation into two types of
bacteria growth and how this was investigated.
Methodology
In the beginning of micro organism cultures we have bacteria in "nature" and how that can
cause "disease", However not all bacteria's cause disease. These types of bacteria have been
given terms such as "germs". This can be equated to :
Pathogens – A disease causing agent – these can operate in two ways -
Break down tissues for food (for example the Tuberculosis pathogen)
Release toxins that can harm the host (for example food poisoning)
Many bacteria can be "killed" or kept in check, with antibiotics or vaccines.
Human causes of bacteria:
In food products that are made or produced through the actions of micro organisms, for
example, cheeses, buttermilk, chocolate, creams, yoghurts. Bacteria's can also be used to
make medicines. Bacteria can also be used in industry to clean up toxic waste.
How to control Bacteria:
Assignment No: 2 Cultiatng Micro-Organisms
Using Aseptic techniques to cultiivte Micro-Orgvnisms
AIM of the Experiment:
To show a method of the lawn plate to show cell culture techniques.
To show and describe methods using aseptic techniques to safely carry out a practical
investigation.
To explain the applications of cell cultures in research and possible development.
To explain & show how valuable aseptic techniques are to this area.
Introduction:
The term "micro-organism" or microbe, refers to any single-celled living organism, including
bacteria, viruses and fungi. Microbes ate transferred by direct contact or indirectly through a
vehicle (e.g. a surgical tool) or via the air a person breathes. If favourable conditions for
growth exist a new host, microbes can establish themselves and reproduce colonies.
Many types of organisms are pathogenic, (foreign body) and when they enter the body, they
cause infection. Here they can damage the cells directly or release toxins that will eventually
cause damage. The prevention of disease-causing microbes especially in hospitals for
example or patient care, is generally accomplished using "Aseptic or sterile techniques".
Here is then the goal is to create a germ free environment as much as is deemed possible.
This is achieved through aseptic techniques and sterilization and the maintenance of
sterile/non-sterile barriers.
In this particular experiment we are showing and explaining the principles underlying the
microbiological techniques used in terms of relevant aseptic techniques and the method of
cultivation.
Within this report of the practical carried out there will be an investigation into two types of
bacteria growth and how this was investigated.
Methodology
In the beginning of micro organism cultures we have bacteria in "nature" and how that can
cause "disease", However not all bacteria's cause disease. These types of bacteria have been
given terms such as "germs". This can be equated to :
Pathogens – A disease causing agent – these can operate in two ways -
Break down tissues for food (for example the Tuberculosis pathogen)
Release toxins that can harm the host (for example food poisoning)
Many bacteria can be "killed" or kept in check, with antibiotics or vaccines.
Human causes of bacteria:
In food products that are made or produced through the actions of micro organisms, for
example, cheeses, buttermilk, chocolate, creams, yoghurts. Bacteria's can also be used to
make medicines. Bacteria can also be used in industry to clean up toxic waste.
How to control Bacteria:
