Cond.hons neccesso Y Par b ecttra pro-t4
icrobiolo7 nulnients Carbon and enerty Sow ce, Mtret ions
Gram- posilie bocferia Gram neqativbacfe ria
edoglycan 38Ppolysolarde
6 d88868 A ollalne
-Tenperat.re (25 °c-45°C)
S885855589 8
Caltwetfiog in bacterio
re unt red hot ad let aol
Pars
Stoiin method
2. LPthd enou to ter
violet lolneoalkohol add Safronin
ad Crystal odd Lgal's 3 Secur
(qram-pesitive purple ) (sold:Pies dya) remos top layor o gram-neg ative pL) pet dish ispe
30m negafive
4. Incubat 25°C n37C beuas sc
pala qeni
boctar a gro at 37C
Counting co lonies
ttaiAuto clae: 12C ste , I5 inuteks
Osin ling berches disinfect wih 3 lysol
total f aekna
Typers bocter o Sperical ( cocuus)
colones oerla p/alun p colory no. x dilten pebr =
too l:Hle colonie s fo be rod shope Cbacillus)
can count accurately
Assunpfion: 4 cel, 1 coloay stat.s tcally signRcsnt.
5Piral spiilum )
icrobiolo7 nulnients Carbon and enerty Sow ce, Mtret ions
Gram- posilie bocferia Gram neqativbacfe ria
edoglycan 38Ppolysolarde
6 d88868 A ollalne
-Tenperat.re (25 °c-45°C)
S885855589 8
Caltwetfiog in bacterio
re unt red hot ad let aol
Pars
Stoiin method
2. LPthd enou to ter
violet lolneoalkohol add Safronin
ad Crystal odd Lgal's 3 Secur
(qram-pesitive purple ) (sold:Pies dya) remos top layor o gram-neg ative pL) pet dish ispe
30m negafive
4. Incubat 25°C n37C beuas sc
pala qeni
boctar a gro at 37C
Counting co lonies
ttaiAuto clae: 12C ste , I5 inuteks
Osin ling berches disinfect wih 3 lysol
total f aekna
Typers bocter o Sperical ( cocuus)
colones oerla p/alun p colory no. x dilten pebr =
too l:Hle colonie s fo be rod shope Cbacillus)
can count accurately
Assunpfion: 4 cel, 1 coloay stat.s tcally signRcsnt.
5Piral spiilum )
