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Method for food testing unit 8

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Method for food testing Unit 8 - Physiology of Human Body Systems

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Method for food testing:

Testing for reducing sugars:
1. Add 2cm of test solution and benedict solution to a boiling tube
2. Heat in a boiling water batch (80 degrees) for 5 minutes
3. Remove the boiling tube and observe the colour of the precipitate formed


Brown to green to orangey brown to orange and brick red will
be among the colours. The final colour should be brick red. If
there are brick red sugars present. People utilise it in added
sugar, dairy products, fruits and vegetables, and it is beneficial
because it allows our bodies to balance a nutritious diet and
absorb all of the nutrients necessary for our bodies to function
effectively and appropriately. Testing for non-reducing sugar:

1. To 2cm of test solution, add two drops of hydrochloric acid. The non-reducing
sugars glyosidic linkages are hydrolysed by HCL, releasing reducing sugars into the
solution.

2. Boil the boiling tube for 2 minutes in a water bath (80 degrees).

3. Set aside to cool

4. Fill the boiling tube with 2 drops of weak sodium hydroxide. The excess HCL is
neutralised as a result of this.

5. Use Benedict Regent to retest the solution.

6. Make a note of the colour of the precipitate that has developed.

The colour progression should be blue to green to brown to
orange to brick red. Bacteria, fungi, yeast, insects, and plants
are all sources of this nutrient.

Testing for proteins:

1. Fill a boiling tube with 2cm of test solution and the biuret reaction.

2. Gently shake to combine.

3. Keep a journal of your observations. (Purple to blue)

It can be found in shellfish, lean meat, eggs, beans, and other foods.

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