STRAIGHTERLINE MICROBIOLOGY LAB
BIO250L FINAL STUDY GUIDE 2026 TESTED
ANSWERS GRADED A+
⩥ define the medium?
Answer: liquid or gel designed to expose bacteria
⩥ what are the two types if medium and what do they provide?
Answer: broth and agar; they provide variable growth conditions in the
form of growth and stability
⩥ what three ways do you handle media?
Answer: make sure that the top is on the bottom; plate is upside down
make sure to seal the plate with parafilm
store in correct temp for the bacteria
⩥ define agar
Answer: chemical derived from seaweed that solidifies into a jelly-like
substance and becomes a solid
⩥ what are two types of agar?
Answer: plates and slants
, ⩥ example of agar?
Answer: TSA--- trypticase soy agar
⩥ what is the air exposure procedure?
Answer: open the plate to expose it to the air for several minutes then
seal with parafilm and place in an incubate at 22C for 24 hours then 4C
until the next week
⩥ what is the objective of a surface swab?
Answer: to demonstrate that their are microorganism and can only kill
some
⩥ what is sterilization and some examples?
Answer: when you kill all the bacteria;
autoclave and radiation
⩥ what is autoclave?
Answer: example of sterilization when you use heat and pressure
⩥ what is disinfection and an example?
Answer: only kills some or most of the microorganisms; Lysol
BIO250L FINAL STUDY GUIDE 2026 TESTED
ANSWERS GRADED A+
⩥ define the medium?
Answer: liquid or gel designed to expose bacteria
⩥ what are the two types if medium and what do they provide?
Answer: broth and agar; they provide variable growth conditions in the
form of growth and stability
⩥ what three ways do you handle media?
Answer: make sure that the top is on the bottom; plate is upside down
make sure to seal the plate with parafilm
store in correct temp for the bacteria
⩥ define agar
Answer: chemical derived from seaweed that solidifies into a jelly-like
substance and becomes a solid
⩥ what are two types of agar?
Answer: plates and slants
, ⩥ example of agar?
Answer: TSA--- trypticase soy agar
⩥ what is the air exposure procedure?
Answer: open the plate to expose it to the air for several minutes then
seal with parafilm and place in an incubate at 22C for 24 hours then 4C
until the next week
⩥ what is the objective of a surface swab?
Answer: to demonstrate that their are microorganism and can only kill
some
⩥ what is sterilization and some examples?
Answer: when you kill all the bacteria;
autoclave and radiation
⩥ what is autoclave?
Answer: example of sterilization when you use heat and pressure
⩥ what is disinfection and an example?
Answer: only kills some or most of the microorganisms; Lysol
