Bio Exam 4 Pearson
Study online at https://quizlet.com/_f9zja8
1. The unpaired nucleotides produced by the action of sticky ends
restriction enzyme are referred to as
2. What information can not be obtained from the se- whether the gene
quence of a gene? is methylated
3. What is the polymerase chain reaction (PCR)? a method to am-
plify a fragment of
DNA
4. Comparison of the sequences of the same gene true
across species can give some insight into the exis-
tence of a common ancestor with that gene.
5. The Taq enzyme is a type of DNA polymerase that al- false: require or
lows researchers to separate the DNA strands during the denaturation
the annealing step of the PCR cycle without destroy- step of PCR
ing the polymerase.
6. How many DNA molecules would there be after four 32
rounds of PCR if the initial reaction mixture contains
two molecules?
7. During which step in the PCR cycle are nucleotides extension
used?
8. During which step in the PCR cycle do primers form annealing
bonds with a single-stranded template?
9. How could they obtain sufficient DNA from a tiny use the poly-
sample for an analysis of the ancient human's genes? merase chain re-
action
10. cDNA is synthesized from what molecule using an mRNa
enzyme known as what? reverse transcrip-
tase
11. Gel electrophoresis separates DNA fragments on the length
basis of what characteristic?
, Bio Exam 4 Pearson
Study online at https://quizlet.com/_f9zja8
12. Why are yeast cells frequently used as hosts for they are eukaryot-
cloning? ic cells
13. Many identical copies of genes cloned in bacteria plasmid and bac-
are produced as a result of which of the following terial cell repro-
processes? duction
14. The final step in a Sanger DNA sequencing reaction it separates DNA
is the run the DNA fragment on a gel. What purpose fragments gener-
does this serve? ated during the
sequencing reac-
tion based on
one-nucleotide dif-
ferences in their
size
15. The defense claims that DNA evidence using STR the prosecution is
analysis with 13 markers is not sufficient to reliably right. STR analy-
distinguish between people. What do you think? sis with 13 mark-
ers gives a 1 in 10
billion or greater
chance that two
people will have
the same pattern
16. Which mutated protein causes the symptoms of sick- hemoglobin
le cell disease?
17. Scientists decide to use this technique to treat cystic use CRISPR to al-
fibrosis. Which of the following do they do? ter the DNA in pa-
tient's cells
18. A second patient has this treatment and it is again de- the patient started
clared a success. Which of the following happened? making fetal he-
moglobin
19. Which of the following is true regarding CRISPR treat- it will have no ef-
ment fect on the chance
of people having
sickle cell disease
Study online at https://quizlet.com/_f9zja8
1. The unpaired nucleotides produced by the action of sticky ends
restriction enzyme are referred to as
2. What information can not be obtained from the se- whether the gene
quence of a gene? is methylated
3. What is the polymerase chain reaction (PCR)? a method to am-
plify a fragment of
DNA
4. Comparison of the sequences of the same gene true
across species can give some insight into the exis-
tence of a common ancestor with that gene.
5. The Taq enzyme is a type of DNA polymerase that al- false: require or
lows researchers to separate the DNA strands during the denaturation
the annealing step of the PCR cycle without destroy- step of PCR
ing the polymerase.
6. How many DNA molecules would there be after four 32
rounds of PCR if the initial reaction mixture contains
two molecules?
7. During which step in the PCR cycle are nucleotides extension
used?
8. During which step in the PCR cycle do primers form annealing
bonds with a single-stranded template?
9. How could they obtain sufficient DNA from a tiny use the poly-
sample for an analysis of the ancient human's genes? merase chain re-
action
10. cDNA is synthesized from what molecule using an mRNa
enzyme known as what? reverse transcrip-
tase
11. Gel electrophoresis separates DNA fragments on the length
basis of what characteristic?
, Bio Exam 4 Pearson
Study online at https://quizlet.com/_f9zja8
12. Why are yeast cells frequently used as hosts for they are eukaryot-
cloning? ic cells
13. Many identical copies of genes cloned in bacteria plasmid and bac-
are produced as a result of which of the following terial cell repro-
processes? duction
14. The final step in a Sanger DNA sequencing reaction it separates DNA
is the run the DNA fragment on a gel. What purpose fragments gener-
does this serve? ated during the
sequencing reac-
tion based on
one-nucleotide dif-
ferences in their
size
15. The defense claims that DNA evidence using STR the prosecution is
analysis with 13 markers is not sufficient to reliably right. STR analy-
distinguish between people. What do you think? sis with 13 mark-
ers gives a 1 in 10
billion or greater
chance that two
people will have
the same pattern
16. Which mutated protein causes the symptoms of sick- hemoglobin
le cell disease?
17. Scientists decide to use this technique to treat cystic use CRISPR to al-
fibrosis. Which of the following do they do? ter the DNA in pa-
tient's cells
18. A second patient has this treatment and it is again de- the patient started
clared a success. Which of the following happened? making fetal he-
moglobin
19. Which of the following is true regarding CRISPR treat- it will have no ef-
ment fect on the chance
of people having
sickle cell disease
