BIOCHEM C785 pre assessment #3

5' TTG TTA CTG 3 3’ GTC ATT GTT 5’ 3’ GTT ATT GTC 5’ 3' TTG TTA CTG 5 The correct answer is 3' TTG TTA CTG 5'. Remember complementary means “Matching or Pairing” You have to remember to pay attention to your numbers as well as your letters (A-T, G- C, 5'-3'). The correct answer is 3’ CAG TAA CAA 5’ (original sequence) 5’ GTC ATT GTT 3’ (complementary sequence) But we asked for it in the 3’ direction, so mirror answer to give correct answer: 3’ TTG TTA CTG 5’ Correct answers: 3' TTG TTA CTG 5 Gln Leu His The correct answer is Gln Leu His. We are starting at the coding strand, and have to remember the relationship between coding DNA and mRNA. These two strands are non-complementary and parallel. So we copy the coding strand , change T ---> U, and then write the mRNA sequence: 3’ TAC GTT AAC 5’ coding 3’ UAC GUU AAC 5’ mRNA Mirror by changing