ABO PRACTICAL KNOWLEDGE ASSESSMENT
WITH ANSWER KEY
◉ What reagents are used in Forward ABO typing?
Answer: Anti-A, Anti-B, Anti-A,B, and patient RBC suspension.
◉ What does agglutination indicate in Forward ABO typing?
Answer: Agglutination indicates that the antigen is present.
◉ What is the purpose of Reverse ABO typing?
Answer: To detect the patient's expected ABO antibodies and
confirm the forward typing.
◉ What reagents are used in Reverse ABO typing?
Answer: A₁ cells and B cells.
◉ What does a positive result in Reverse ABO typing indicate?
Answer: A positive result indicates that the corresponding antibody
exists in the plasma.
◉ What is the purpose of Rh typing?
, Answer: To determine whether the patient's RBCs possess the D
antigen.
◉ What must be negative for the Rh result to be trusted?
Answer: The Rh control must be negative.
◉ What does a positive Rh control indicate?
Answer: It indicates nonspecific agglutination and that the Rh result
is invalid.
◉ What is the critical rule for Rh typing?
Answer: Rh control must be negative to trust the Rh result.
◉ What is the purpose of the Antibody Screen?
Answer: To detect unexpected clinically significant antibodies in
patient plasma/serum.
◉ Why are Group O cells used in the Antibody Screen?
Answer: Because Group O cells do not have A or B antigens that
would interfere with the test.
◉ What is the sequence of steps in the Antibody Screen procedure?
WITH ANSWER KEY
◉ What reagents are used in Forward ABO typing?
Answer: Anti-A, Anti-B, Anti-A,B, and patient RBC suspension.
◉ What does agglutination indicate in Forward ABO typing?
Answer: Agglutination indicates that the antigen is present.
◉ What is the purpose of Reverse ABO typing?
Answer: To detect the patient's expected ABO antibodies and
confirm the forward typing.
◉ What reagents are used in Reverse ABO typing?
Answer: A₁ cells and B cells.
◉ What does a positive result in Reverse ABO typing indicate?
Answer: A positive result indicates that the corresponding antibody
exists in the plasma.
◉ What is the purpose of Rh typing?
, Answer: To determine whether the patient's RBCs possess the D
antigen.
◉ What must be negative for the Rh result to be trusted?
Answer: The Rh control must be negative.
◉ What does a positive Rh control indicate?
Answer: It indicates nonspecific agglutination and that the Rh result
is invalid.
◉ What is the critical rule for Rh typing?
Answer: Rh control must be negative to trust the Rh result.
◉ What is the purpose of the Antibody Screen?
Answer: To detect unexpected clinically significant antibodies in
patient plasma/serum.
◉ Why are Group O cells used in the Antibody Screen?
Answer: Because Group O cells do not have A or B antigens that
would interfere with the test.
◉ What is the sequence of steps in the Antibody Screen procedure?
