BIOCHEM C785 FINAL FULL SOLUTION STUDY
GUIDE
◉ In the first step of glycolysis, the given two reactions are coupled.
reaction 1: glucose+Pi⟶glucose-6-phosphate+H2O
Δ𝐺= +13.8 kJ/mol
reaction 2:ATP+H2O⟶ADP+Pi
Δ𝐺= −30.5 kJ/molreaction
1. Is reaction 2 spontaneous or nonspontaneous?
2. Complete the net chemical equation.
3. Calculate the overall ΔG for the coupled reaction.
4. Is the first step in glycolysis spontaneous (favorable)?
Answer: 1. spontaneous
2. ATP + glucose ⟶ glucose-6-phosphate + ADP
,3. reaction 1Δ𝐺 = +13.8
reaction 2Δ𝐺 = −30.5
overall Δ𝐺 = −16.7 kJ/mol
4. yes
◉ Hexokinase catalyzes the first step of glycolysis, in which glucose
is phosphorylated to form glucose-6-phosphate.
Which of these statements are accurate?
Answer: - Hexokinase is a type of transferase that catalyzes the
transfer of a phosphoryl group from ATP to a hexose.
- Hexokinase consists of two domains, or lobes, that come together
when glucose and the
- The conformational shift that occurs when glucose, but not water,
enters the active site prevents water from hydrolyzing ATP.
◉ Hexokinase is an enzyme that catalyzes the ATP-dependent
phosphorylation of glucose to glucose 6-phosphate. Watch the
animation of hexokinase converting glucose into glucose
6-phosphate and answer the questions.
,1. Modify the glucose molecule to glucose 6-phosphate.
2. The induced-fit model describes the interaction between the
hexokinase and glucose.
Answer: 1. Ans Ch 14 Question 5
2. Induced fit model
◉ Refer to the glycolytic pathway to answer the question.
Identify the oxidation-reduction reactions of glycolysis. Use the step
numbers from the glycolytic pathway shown.
Answer: step 6
◉ The passage describes some glycolysis reactions. Select the
appropriate term for each blank to complete the passage.
In the first reaction of glycolysis, glucose is converted to glucose
6-phosphate. The phosphate comes from ATP. A kinase is an enzyme
that transfers the terminal phosphate of ATP to a substrate. The
product of this reaction is then isomerized to fructose-6-phosphate.
Fructose-6-phosphate is then phosphorylated by a second
kinase reaction, giving fructose 1,6-bisphosphate .
Answer: glucose 6-phosphate
, ATP
Kinase
ATP
Isomerized
Kinas
Fructose 1,6-biphosphate
◉ Complete the aldolase reaction of glycolysis by drawing the
product or products.
Which enzyme catalyzes the next reaction, interconverting two
trioses?
Answer: 1. CH 14 Q 8
2. triose phosphate isomerase
◉ Complete the glycolysis reaction.
What is the enzyme that catalyzes this reaction?
Answer: CH 14 Q 9
1. ADP - ATP
GUIDE
◉ In the first step of glycolysis, the given two reactions are coupled.
reaction 1: glucose+Pi⟶glucose-6-phosphate+H2O
Δ𝐺= +13.8 kJ/mol
reaction 2:ATP+H2O⟶ADP+Pi
Δ𝐺= −30.5 kJ/molreaction
1. Is reaction 2 spontaneous or nonspontaneous?
2. Complete the net chemical equation.
3. Calculate the overall ΔG for the coupled reaction.
4. Is the first step in glycolysis spontaneous (favorable)?
Answer: 1. spontaneous
2. ATP + glucose ⟶ glucose-6-phosphate + ADP
,3. reaction 1Δ𝐺 = +13.8
reaction 2Δ𝐺 = −30.5
overall Δ𝐺 = −16.7 kJ/mol
4. yes
◉ Hexokinase catalyzes the first step of glycolysis, in which glucose
is phosphorylated to form glucose-6-phosphate.
Which of these statements are accurate?
Answer: - Hexokinase is a type of transferase that catalyzes the
transfer of a phosphoryl group from ATP to a hexose.
- Hexokinase consists of two domains, or lobes, that come together
when glucose and the
- The conformational shift that occurs when glucose, but not water,
enters the active site prevents water from hydrolyzing ATP.
◉ Hexokinase is an enzyme that catalyzes the ATP-dependent
phosphorylation of glucose to glucose 6-phosphate. Watch the
animation of hexokinase converting glucose into glucose
6-phosphate and answer the questions.
,1. Modify the glucose molecule to glucose 6-phosphate.
2. The induced-fit model describes the interaction between the
hexokinase and glucose.
Answer: 1. Ans Ch 14 Question 5
2. Induced fit model
◉ Refer to the glycolytic pathway to answer the question.
Identify the oxidation-reduction reactions of glycolysis. Use the step
numbers from the glycolytic pathway shown.
Answer: step 6
◉ The passage describes some glycolysis reactions. Select the
appropriate term for each blank to complete the passage.
In the first reaction of glycolysis, glucose is converted to glucose
6-phosphate. The phosphate comes from ATP. A kinase is an enzyme
that transfers the terminal phosphate of ATP to a substrate. The
product of this reaction is then isomerized to fructose-6-phosphate.
Fructose-6-phosphate is then phosphorylated by a second
kinase reaction, giving fructose 1,6-bisphosphate .
Answer: glucose 6-phosphate
, ATP
Kinase
ATP
Isomerized
Kinas
Fructose 1,6-biphosphate
◉ Complete the aldolase reaction of glycolysis by drawing the
product or products.
Which enzyme catalyzes the next reaction, interconverting two
trioses?
Answer: 1. CH 14 Q 8
2. triose phosphate isomerase
◉ Complete the glycolysis reaction.
What is the enzyme that catalyzes this reaction?
Answer: CH 14 Q 9
1. ADP - ATP
