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MOLECULAR DIAGNOSTICS FINAL TEST QUESTIONS AND ANSWERS Calculate the DNA concentration in ug/mL from the following information: Absorbance reading at 260 nm from a 1:50 dilution = 0.307 - ANSWER-767.5 ug/mL Calculate the DNA concentration in ug/mL from the following information: Absorbance reading at 260 nm from a 1:100 dilution = 0.172 - ANSWER-860 ug/mL Calculate the DNA concentration in ug/mL from the following information: Absorbance reading at 260 nm from a 1:100 dilution = 0.08...

MOLECULAR DIAGNOSTICS FINAL (EXAM + QUIZ MC) QUESTIONS AND ANSWERS c (primer annealing and extension) - ANSWER-*Fluorescence is detectable at which stage of the real-time PCR process?* a. Template denaturation b. Cycle 1 c. Primer annealing and extension d. Prior to amplification c (3) - ANSWER-*A circular molecule has two sites for the restriction enzyme PstI and one site for the enzyme BamH1. How many fragments will result when digested with both enzymes?* a. 1 b. 2 c. 3 d. 4 ...

MOLECULAR DIAGNOSTICS EXAM SET QUESTIONS AND ANSWERS What are some of the limitations of molecular genetic testing? - ANSWER-Most therapeutic targets are phenotype (don't need to know genotype to treat phenotype), Mutations may not predict phenotype (have mutation but not have disease state) Genetic mutation in the absence of symptoms Explain the concept of penetrance - ANSWER-Frequency gene is expressed, can be incomplete (people have mutation but don't have phenotype) Describe t...

ROBOTICS TEST STUDY GUIDE QUESTIONS AND ANSWERS Specifications - Answer-A detailed description of the design and materials used to make something. Troubleshoot - Answer-The process of locating issues and making repairs in machinery and technical equipment. Algorithm - Answer-A methodical, logical rule or procedure that guarantees solving a particular problem. Prototype - Answer-an original model on which later versions are patterned Robot - Answer-A machine capable of carrying out a comple...

MOLECULAR DIAGNOSTICS EXAM #3 QUESTIONS WITH COMPLETE ANSWERS STR analysis is performed on bone marrow donor-recipient pairs prior to transplantation for which of the following reasons? - ANSWER-To find informative alleles for post-transplantation studies What is a microvariant? - ANSWER-An STR Allele missing base pairs in the repeat unit. What is an expression for how many times morelikely the child's allele is inherited from the alleged father than by random occurence of the allele...

MOLECULAR DIAGNOSTICS EXAM REVIEW QUESTIONS AND ANSWERS What helps to visualize the sample while electrophoresis is taking place? - ANSWER-Loading dye (buffer) What types of loading dyes are available? - ANSWER--Ethidium bromide (carcinogenic) -Fast Blast What are the contents of the loading dye? - ANSWER--10 to 20% glycerol -Tracking dye Polyacrylamide Gel Electrophoresis - ANSWER-PAGE PAGE composition: - ANSWER-Cross-linked polyacrylamide polymer PAGE pore uniformity: - ANS...

MOLECULAR DIAGNOSTICS EXAM #2 QUESTIONS WITH CORRECT ANSWERS p. 110 nucleic acid specific dyes - SYBR green - ANSWER-SyBr green in association with DNA or RNA also emits light in the orange range (522 nm). In agarose gel electrophoresis, SyBr staining is 25-100 times more sensitive than EtBr (detection level: 60 pg of double-stranded DNA vs. 5 ng for EtBr). This is due, in part, to background fluorescence from EtBr in agarose. SyBr green can also be added directly to the DNA sample before ...

MOLECULAR DIAGNOSTICS EXAM 2 MATERIAL QUESTIONS AND ANSWERS If DNA is not pure, then it is usually contaminated with _____________ or ________________ from the DNA isolation reagents. - ANSWER-protein. inorganic/organic compounds. Protein absorbs maximally at _____________ nm. Contaminants (EDTA, phenol, Guanidine salts) absorb maximally at ____________ nm. - ANSWER-A280 A230. To determine DNA purity from protein contamination, you can take the A260/A280 to get a ratio. It should...

MOLECULAR DIAGNOSTICS PRACTICE EXAM QUESTIONS AND ANSWERS Nucleotide - ANSWER-This is composed of: -A 5-carbon sugar -Phosphate group -Nitrogenous base Nucleoside - ANSWER--This is composed of: -A 5-carbon sugar -Nitrogenous base -*NO phosphate group* Phosphodiester bond - ANSWER--This bond forms between the 3' hydroxyl group of the last nucleotide and the 5' phosphate group of an incoming nucleotide RNA - ANSWER--Has an -OH on the 2nd sugar -Single-stranded -Ribose sug...

MOLECULAR DIAGNOSTICS EXAM Q&A Messenger RNA is different from other types of RNA because messenger RNA has a. a cruciform structure. b. a 3' methylated cap. c. a 3' polyA tail. d. introns and exons. - ANSWER-a 3' polyA tail. A mechanism of intron removal results in which by-product? a. PolyA tails b. Dinucleotides c. Okazaki fragments d. Lariats - ANSWER-Lariats Thalassemias arise from changes in what part of the beta-globin gene? a. 3' untranslated region...

MOLECULAR DIAGNOSTICS EXAM AND ANSWERS semiconservative - ANSWER-Semi-conservative replication is the key to maintaining the sequence of the nucleotides in DNA through new generations. Every cell in a multicellular organism or in a clonal population of unicellular organisms carries the same genetic information. It is important that this information, in the form of the DNA sequence, be transferred faithfully at cell division. The replication apparatus is designed to copy the DNA strands in...

MOLECULAR DIAGNOSTICS EXAM 1 QUESTIONS AND ANSWERS 2025 / 2026 Translation is terminated when a stop codon is presented at the; - ANSWER-A site According to the wobble hypothesis a U in the 5' position of anticodon can pair with; - ANSWER-A or G Which enzyme is incorrectly matched to it's function? -RNA polymerase; transcription -Aminoacyl-tRNA synthesis; attaches an amino acid to a tRNA -PolyA polymerase; translation -Guanlyl transferase- mRNA capping - ANSWER-Poly A polymera...

MOLECULAR DIAGNOSTICS EXAM CHAPTER #1 QUESTIONS AND ANSWERS In a DNA molecule, one end has a free hydroxyl group and one end has a free what? - ANSWER-phosphate group The nucleotides in single strand of DNA are held together by _________________ bonds between sugar and phosphate groups - ANSWER-phosphodiester Which of the five ribose carbons of the deoxyribonucleotide are involved in formation of the DNA chain? A. 1' and 5' B. 2' and 4' C. 3' and 4' D. 3' and 5...

MOLECULAR DIAGNOSTICS CHAPTER 1-16 EXAM QUESTIONS WITH COMPLETE ANSWERS An exonuclease will catalyze what type of reaction? - ANSWER-Dissociation of phosphodiester bonds at the ends of DNA molecules What reactions are catalyzed by DNA methyltransferase? - ANSWER-Addition of methyl groups to nitrogen bases The purpose of ligase is to what? - ANSWER-Paste 2 ends of DNA together What is the fertility factor (F) observed by Hayes and Lederberg? - ANSWER-A plasmid Avery, MacLeod, and M...

MOLECULAR DIAGNOSTICS CHAPTER #1 EXAM QUESTIONS WITH CORRECT ANSWERS What reactions are catalyzed by DNA methyltransferase? - ANSWER-Addition of methyl groups to nitrogen bases The purpose of ligase is to what? - ANSWER-Paste 2 ends of DNA together What is the fertility factor (F) observed by Hayes and Lederberg? - ANSWER-A plasmid Avery, MacLeod, and McCarty identified the nature of Griffith's transforming factor in an experiment where it was destroyed by what treatment? - ANSWER...

MODULE 3 TEST BANK EXAM Q&A How do formal and informal power differ? - Answer-Formal: Legitimate (Authority) Reward/Coercive (Resources) Informal: Referent (Social Standing) Informational (Knowledge) Name some ways to increase formal power - Answer-Climb the ladder Gain control of critical resources Name some ways to increase informal power - Answer-Credentials Experience in the organization Abilities and Knowledge Name and briefly describe the five four theoretical bases o...

MODULE 3 TEST BANK QUESTIONS AND ANSWERS Dysfunctional: Unhealthy responses to conflict impedes... coordination of effort ... and achievement of group goals. What kinds of outcomes does functional (i.e., healthy) conflict resolution yield more specifically? - Answer-decision-making quality and collective performance. How do we define power for this course? - Answer-Capacity to assert your will on others Name three ways having and not having power affects people. - Answer-Having power: ...

TAP Travel Module #2 Exam Questions and Answers How does the Supervision and Educational Requirements mechanism protect consumers? - Answer-require personnel to have a certain level of expertise (updated to on July 1, 2009) True or False? If an Ontario tour operator goes bankrupt, consumers who purchased their vacation from any travel agency can make a claim. - Answer-false. If an Ontario tour operator goes bankrupt, only consumers who purchased their vacation from a licensed Ontario tra...

MICROBIOLOGY CHAPTER 11 TEST BANK EXAM QUESTIONS AND ANSWERS Burkholderia was reclassified from the gammaproteobacteria to the betaproteobacteria because A) it grows in disinfectants. B) it is a gram-negative rod. C) it causes infections in cystic fibrosis patients. D) it causes melioidosis. E) its rRNA sequence is similar to that of Neisseria. - Answer-its rRNA sequence is similar to that of Neisseria The crab industry needs female crabs for growing more crabs. What bacterium might b...

MICROBIOLOGY CHAPTER 10 TEST BANK EXAM QUESTIONS AND ANSWERS Western blotting is an immunological test used to identify the antibodies in the serum of an individual suspected of having Lyme disease. - Answer-TRUE When building a cladogram, the length of the horizontal branch is based on the calculated percent similarity between organisms. - Answer-TRUE Which of the following statements about archaea is FALSE? A) They are prokaryotes. B) They lack peptidoglycan in their cell walls. C) ...