MGG Exam 4 questions well answered
graded A+ 2025/2026
A FASTA file always begins with a:
a. <
b. @
c. /
d. > - correct answer ✔✔D
Besides the > sign, the first line of a FASTA file includes:
a. the read quality data
b. the sequence
c. the accession number for the sequence
d. the type of sequencing equipment used in obtaining the sequence - correct answer ✔✔C
The second line of a FASTA file comprises:
a. the sequence
b. the read quality data
c. the accession number for the sequence
d. the type of sequencing equipment used in obtaining the sequence - correct answer ✔✔A
A FASTQ file always begins with a:
a. <
b. >
c. @
, d. / - correct answer ✔✔C
The second line of a FASTQ file comprises:
a.the read quality data
b. the accession number for the sequence
c. the sequence
d. the type of sequencing equipment used in obtaining the sequence - correct answer ✔✔C
The fourth line of FASTQ line comprises:
a. the accession number for the sequence
b. the type of sequencing equipment used in obtaining the sequence
c. the read quality data
d. the sequence - correct answer ✔✔C
A PHRED score of 40 for a particular base call corresponds to the probability of 1 base call error
in how many base calls? [Note: this is a Review Quiz question; you won't be asked to memorize
the formula for the exam]
a. 40
b. 4,000
c. 10,000
d. 40,000 - correct answer ✔✔C
What does a PHRED score of 30 or above indicate?
a. Low error rate for the entire read; acceptable quality
b. Low error rate for that base call; acceptable quality
c. High error rate for that base call; unacceptable quality
d. High error rate for the entire read; unacceptable quality - correct answer ✔✔B
graded A+ 2025/2026
A FASTA file always begins with a:
a. <
b. @
c. /
d. > - correct answer ✔✔D
Besides the > sign, the first line of a FASTA file includes:
a. the read quality data
b. the sequence
c. the accession number for the sequence
d. the type of sequencing equipment used in obtaining the sequence - correct answer ✔✔C
The second line of a FASTA file comprises:
a. the sequence
b. the read quality data
c. the accession number for the sequence
d. the type of sequencing equipment used in obtaining the sequence - correct answer ✔✔A
A FASTQ file always begins with a:
a. <
b. >
c. @
, d. / - correct answer ✔✔C
The second line of a FASTQ file comprises:
a.the read quality data
b. the accession number for the sequence
c. the sequence
d. the type of sequencing equipment used in obtaining the sequence - correct answer ✔✔C
The fourth line of FASTQ line comprises:
a. the accession number for the sequence
b. the type of sequencing equipment used in obtaining the sequence
c. the read quality data
d. the sequence - correct answer ✔✔C
A PHRED score of 40 for a particular base call corresponds to the probability of 1 base call error
in how many base calls? [Note: this is a Review Quiz question; you won't be asked to memorize
the formula for the exam]
a. 40
b. 4,000
c. 10,000
d. 40,000 - correct answer ✔✔C
What does a PHRED score of 30 or above indicate?
a. Low error rate for the entire read; acceptable quality
b. Low error rate for that base call; acceptable quality
c. High error rate for that base call; unacceptable quality
d. High error rate for the entire read; unacceptable quality - correct answer ✔✔B