BIOL 2444 MICRO LAB FINAL EXAM QUESTIONS WITH CORRECT ANSWERS LATEST UPDATE 2025/2026

BIOL 2444 MICRO LAB FINAL EXAM QUESTIONS WITH CORRECT ANSWERS LATEST UPDATE 2025/2026 undefined media-1 - Answers exact ingredient concentrations unknown defined media-1 - Answers all ingredients are purified and precisely known when added to prepare the media sterilization-1 - Answers process that eliminates or deactivates all biological agents (bacteria, fungi, viruses, etc.) autoclave settings-1 - Answers 121 degree Celcius and 15 PSI 3 phases of autoclave cycle-1 - Answers purge, sterilization, exhaust purge phase-1 - Answers steam flows into the chamber and replaces air with steam, pressure is ramped up slowly sterilizaton phase-1 - Answers exhaust valve is closed causing the temperature and pressure to reach the desired setpoint exhaust phase-1 - Answers pressure is slowly released from the chamber through an exhaust valve until ambient pressure is reached pathogenic relationship-1 - Answers harmful to host communalistic relationship-1 - Answers neutral to host mutualistic relationship-1 - Answers beneficial to host microbiocidal-1 - Answers kill microbes microbiostatic-1 - Answers inhibit microbe growth condenser-1 - Answers creates a condensed light of beam objective lens-1 - Answers The lens on a light microscope that is closest to the stage. 4-100X ocular lens-1 - Answers the lens on a light microscope that your eyes look through. 10X compound microscope-1 - Answers a microscope that uses 2 or more lenses total magnification-1 - Answers objective lens x ocular lens resolution-1 - Answers clarity of the specimen image, goes down with higher levels of magnification numerical aperture-1 - Answers measure of a lens ability to "capture" light coming from the specimen and use it to make the image immersion oil-1 - Answers substance that can be used on the higher power objective lenses such as 100X to aid in capturing light course knob-1 - Answers only used on 4X and 10X fine knob-1 - Answers only used on 40X and 100X binary fission-2 - Answers divides exponentially until nutrients are depleted colony forming unit-2 - Answers cluster of cells visible to the naked eye 3 categories for describing colonies-2 - Answers form, elevation, margin streak plate isolation technique-2 - Answers diluting the polymicrobial mixture until a single bacterial cell or CFU can be obtained, T-streak and Quad-streak are commonly used basic stains-3 - Answers stains that have a positive charge and attracted to negatively charged cell walls, include methylene blue, crystal violet, safranin acidic stains-3 - Answers stains that have a negative charge and attracted to positively charged cell walls, include eosin, India ink, acid fuschin simple staining-3 - Answers the use of a single basic stain differential staining-3 - Answers the use of more than one stain bacterial smear-3 - Answers bacterial emulsion is added to a clean slide and then gently heated over a flame to allow fixation primary stain in gram stain-3 - Answers crystal violet mordant in gram stain-3 - Answers iodine counterstain in gram stain-3 - Answers safranin order of gram stain-3 - Answers crystal violet, iodine, alcohol decolorizer, safranin gram-positive-3 - Answers retain the crystal violet color gram-negative-3 - Answers lose crystal violet to the alcohol decolorizer and then pick