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Phlebotomy Certification Exam Study Guide 2025 | NHA, ASCP & NCCT Prep

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Prepare for your Phlebotomy Certification Exam (NHA, ASCP, NCCT) with this comprehensive 2025 study guide. Covers venipuncture techniques, order of draw, specimen handling, safety protocols, and medical terminology to help you pass your CPT exam and launch your healthcare career.

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Page 1 of 56


Phlebotomy Certification Exam_Latest 2025 100%

Correct

Coagulation tubes have which color stopper? .....ANSWER.....light

blue

Hematology tubes have which color stopper?

.....ANSWER.....purple/lavender

Serum tubes have which color stopper? .....ANSWER.....red/tiger

top/gold

A health care-acquired infections are called?

.....ANSWER.....Nosocomial infections

A inherited condition that is caused by the body's inability to

metabolize phenylalanine (protein) is called: .....ANSWER.....PKU

A lavender stoppered evacuated tube must be redrawn because

of clots in the tube. What collection error causes this?

.....ANSWER.....insufficient mixing of additive with specimen

,Page 2 of 56


A patient has undergone a left radical mastectomy. What site

can not be used for venipuncture until the patient's physician is

consulted? .....ANSWER.....left arm

A patient suffers a transfusion reaction. What may this condition

cause in the patient's specimen? .....ANSWER.....hemolysis

A phlebotomist is to collect a specimen for an APTT and

prothrombin time from a patient. According to the NCCLS

guidelines, what tube stopper color should the phlebotomist

collect FIRST? .....ANSWER.....red

A phlebotomist enters a patient's room at 5:30 am, but the

patient is asleep. The phlebotomist should: .....ANSWER.....gently

wake the patient and proceed

A phlebotomist is about to collect a blood specimen from a

patient in ICU, but the patient is unconscious. The phlebotomist

should: .....ANSWER.....proceed as though the patient were

conscious

,Page 3 of 56


After performing a routine venipuncture, the phlebotomist noticed

that the blood in the evacuated tube was bright cherry red.

What vessel did the phlebotomist puncture? .....ANSWER.....artery

After puncturing a finger during a skin puncture procedure, what

is the next step? .....ANSWER.....Wipe away the first drop with

gauze.

Agranulocytes that function in antibody production are called:

.....ANSWER.....lymphocytes

An evacuated tube contains clot activator and serum separator

gel. What color is the Hemogard TM closure? .....ANSWER.....gold

An example of a specimen that requires protection from light is:

.....ANSWER.....Billirubin, beta-carotene and porphyrins.

Anti diuretic hormone (ADH) requires which of the following

blood collection tubes for proper test results?

.....ANSWER.....purple

, Page 4 of 56


At least how long should a warmer be applied to a skin puncture

site prior to making a skin puncture? .....ANSWER.....3 minutes

Before starting a bleeding time test, the phlebotomist should:

.....ANSWER.....ask the patient if he or she has taken any

medications

Bleeding time is used to access what? .....ANSWER.....platelet

function, blood vessel integrity

Bleeding time procedures are usually ordered:

.....ANSWER.....pre-surgically

Blood collection for neonatal screening to detect metabolic and

genetic abnormalities usually occurs: .....ANSWER.....by collecting

from the lateral plantar surface of the heel.

Blood enters the right side of the heart from what blood vessel?

.....ANSWER.....superior vena cava
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