Page 1 of 94
EXAM 3: BIO322 / BIO 322 (LATEST 2025 UPDATE)
APPLIED PATHOPHYSIOLOGY EXAM | QUESTIONS
AND VERIFIED ANSWERS | 100% CORRECT | GRADE
A - GCU
Which of the following does NOT apply to the reaction
catalyzed by the pyruvate dehydrogenase complex?
.....ANSWER.....Each intermediate in the five step reaction is able
to diffuse to the next active site in a sequential manner.
Which of the following enzymes catalyzes a reaction of the citric
acid cycle that does NOT produce reduced electron carriers?
.....ANSWER.....Succinyl-CoA synthetase
All of the enzymes of the citric acid cycle are located in the
mitochondrion. .....ANSWER.....True
The citric acid cycle is classed as a reductive pathway as it
produces reduced electron carriers. .....ANSWER.....False
,Page 2 of 94
Acetyl CoA is a feed forward activator of the enzyme ________
ensuring sufficient oxaloacetate for the citric acid cycle to
continue. .....ANSWER.....Pyruvate carboxylase
Taking one mole of glucose through glycolysis and the citric acid
cycle generates: .....ANSWER.....6C02, 10 NADH/H+, 2FADH2
and 4 ATP.
ATP synthase can produce ATP using as a direct energy source:
.....ANSWER.....Energy from a proton gradient established across
the inner mitochondrial membrane
Which of the following is correct concerning the mitochondrial
ATP synthase? .....ANSWER.....It catalyzes ATP synthesis by a
rotational catalysts mechanism
During oxidative phosphorylation, the proton motive force that is
generated by electron transport is used to: .....ANSWER.....drive
the synthesis of ATP as protons flow passively back into the
matrix through a proton pore assiciated with ATP synthase
,Page 3 of 94
Which of the following statements is FALSE?
-All of the protein complexes of the mitochondrial respiratory
chain are bound in the inner mitochondrial membrane
-Cytochrome c and coenzyme Q are both soluble electron
carriers that are loosely attached to the outside of the inner
mitochondrial membrane.
-Oxygen is reduced to water at complex IV of the mitochondrial
respiratory chain.
-During electron transport protons are pumped across the inner
mitochondrial membrane from the matrix to the intermembrane
space. .....ANSWER.....Cytochrome c and coenzyme Q are both
soluble electron carriers that are loosely attached to the outside
of the inner mitochondrial membrane.
, Page 4 of 94
Which of the following is NOT an electron acceptor in the
mitochondrial respiratory chain?
CoQH2
FMN
Fe3+
FAD .....ANSWER.....CoQH2
Electron transport through NADH-Coenzyme Q reductase
(Complex I) is accompanied by transfer of ________ protons
from the matrix to the intermembrane space. (answer with the
number. e.g.: 6) .....ANSWER.....4
How do the mitochondria maintain the chemiosmotic gradient
used for the electron transport chain? .....ANSWER.....They
continuously pump protons from the mitochondrial matrix into the
intermembrane space
EXAM 3: BIO322 / BIO 322 (LATEST 2025 UPDATE)
APPLIED PATHOPHYSIOLOGY EXAM | QUESTIONS
AND VERIFIED ANSWERS | 100% CORRECT | GRADE
A - GCU
Which of the following does NOT apply to the reaction
catalyzed by the pyruvate dehydrogenase complex?
.....ANSWER.....Each intermediate in the five step reaction is able
to diffuse to the next active site in a sequential manner.
Which of the following enzymes catalyzes a reaction of the citric
acid cycle that does NOT produce reduced electron carriers?
.....ANSWER.....Succinyl-CoA synthetase
All of the enzymes of the citric acid cycle are located in the
mitochondrion. .....ANSWER.....True
The citric acid cycle is classed as a reductive pathway as it
produces reduced electron carriers. .....ANSWER.....False
,Page 2 of 94
Acetyl CoA is a feed forward activator of the enzyme ________
ensuring sufficient oxaloacetate for the citric acid cycle to
continue. .....ANSWER.....Pyruvate carboxylase
Taking one mole of glucose through glycolysis and the citric acid
cycle generates: .....ANSWER.....6C02, 10 NADH/H+, 2FADH2
and 4 ATP.
ATP synthase can produce ATP using as a direct energy source:
.....ANSWER.....Energy from a proton gradient established across
the inner mitochondrial membrane
Which of the following is correct concerning the mitochondrial
ATP synthase? .....ANSWER.....It catalyzes ATP synthesis by a
rotational catalysts mechanism
During oxidative phosphorylation, the proton motive force that is
generated by electron transport is used to: .....ANSWER.....drive
the synthesis of ATP as protons flow passively back into the
matrix through a proton pore assiciated with ATP synthase
,Page 3 of 94
Which of the following statements is FALSE?
-All of the protein complexes of the mitochondrial respiratory
chain are bound in the inner mitochondrial membrane
-Cytochrome c and coenzyme Q are both soluble electron
carriers that are loosely attached to the outside of the inner
mitochondrial membrane.
-Oxygen is reduced to water at complex IV of the mitochondrial
respiratory chain.
-During electron transport protons are pumped across the inner
mitochondrial membrane from the matrix to the intermembrane
space. .....ANSWER.....Cytochrome c and coenzyme Q are both
soluble electron carriers that are loosely attached to the outside
of the inner mitochondrial membrane.
, Page 4 of 94
Which of the following is NOT an electron acceptor in the
mitochondrial respiratory chain?
CoQH2
FMN
Fe3+
FAD .....ANSWER.....CoQH2
Electron transport through NADH-Coenzyme Q reductase
(Complex I) is accompanied by transfer of ________ protons
from the matrix to the intermembrane space. (answer with the
number. e.g.: 6) .....ANSWER.....4
How do the mitochondria maintain the chemiosmotic gradient
used for the electron transport chain? .....ANSWER.....They
continuously pump protons from the mitochondrial matrix into the
intermembrane space
