PHLEBOTOMY ALL MODULES QUESTIONS AND ANSWERS

PHLEBOTOMY ALL MODULES QUESTIONS AND ANSWERS




The Order of Draw - (ANSWER)the order in which blood must be collected via additive/color. The order
of draw may change depending on your lab, facility, manufacturer or workplace procedure. Below is the
order of draw with additional information. Some of these tubes are rarely used in everyday outpatient
labs but it is good for you to know.



additive - (ANSWER)Nutrient AgarAerobic BEFORE Anaerobic



mode of action - (ANSWER)Preserves viability of microorganisms, grows bacteria



uses - (ANSWER)Microbiology - aerobes, anaerobes, fungi, bacteria cultures Requires BUTTERFLY needle
and hub.



Yellow- Black Top Tube - (ANSWER)Additive: Broth mixtureMode of Action: Preserves viability of
microorganismsUses: Microbiology - aerobes, anaerobes, fungi



Yellow Top Tube - (ANSWER)Additive: ACD (acid-citrate-dextrose) (Solution A or B)



Mode of Action: Complement inactivation *May also go last in the order of draw



Uses: HLA tissue typing, paternity testing, DNA studies, Blood Cultures



Light-Blue Top Tube - (ANSWER)Additive: Sodium citrate



Mode of Action: Forms calcium salts to remove calcium



Uses: Coagulation tests (protime and prothrombin time), full draw required



Red Top Tube - (ANSWER)Additive: None

,PHLEBOTOMY ALL MODULES QUESTIONS AND ANSWERS




Mode of Action: Blood clots, and the serum is separated by centrifugation



Uses: Chemistries, Immunology and Serology, Blood Bank (Crossmatch)



Gold Top (SST) Tube - (ANSWER)Additive: Clot Activator and Thixotropic Gel



Mode of Action: Serum separator tube (SST) contains a gel at the bottom to separate blood from serum
on centrifugation



Uses: Chemistries, Immunology and Serology



Red/Gray Marble Top (SST) Tube - (ANSWER)Additive: Clot Activator and Thixotropic Gel



Mode of Action: Serum separator tube (SST) contains a gel at the bottom to separate blood from serum
on centrifugation



Uses: Chemistries, Immunology and Serology



Orange Top Tube - (ANSWER)Additive: Thrombin



Mode of Action: Quickly clots blood



Uses: STAT serum chemistries



Light Green Top Tube - (ANSWER)Additive: Plasma Separating Tube (PST) with Lithium Heparin



Mode of Action: Anti-coagulates with lithium heparin; Plasma is separated with PST gel at the bottom of
the tube

,PHLEBOTOMY ALL MODULES QUESTIONS AND ANSWERS




Uses: Chemistries



Dark Green Top Tube - (ANSWER)Additive: Sodium heparin



Mode of Action: Inactivates thrombin and thromboplastin



Uses: For lithium level, use sodium heparinFor ammonia level, use sodium or lithium heparin



Lavender Top Tube - (ANSWER)Additive: Dipotassium EDTA



Mode of Action: Forms calcium salts to remove calcium



Uses: Hematology (CBC) and Blood Bank (Crossmatch); requires full draw - invert 8 times to prevent
clotting and platelet clumping



Pink Top Tube - (ANSWER)Additive: Potassium EDTA



Mode of Action: Forms calcium salts



Uses: Immunohematology



White Top Tube - (ANSWER)Additive: Potassium EDTA



Mode of Action: Forms calcium salts



Uses: Molecular/PCR and bDNA testing

, PHLEBOTOMY ALL MODULES QUESTIONS AND ANSWERS




Dark Blue Top Tube - (ANSWER)Additive: EDTA



Mode of Action: Tube is designed to contain no contaminating metals



Uses: Trace element testing (zinc, copper, lead, mercury) and toxicology



Light Brown Top Tube - (ANSWER)Additive: EDTA



Mode of Action: Inactivates thrombin and thromboplastin; contains virtually no lead



Uses: Serum lead determination



Black ESR Vacuum Top Tube - (ANSWER)Additive: 3.2% Sodium Citrate (buffered)



Mode of Action: Forms calcium salts to remove calcium



Uses: Westergren Sedimentation Rate; requires full draw



Light Gray Top Tube - (ANSWER)Additive: Sodium fluoride and potassium oxalate



Mode of Action: Antiglycolytic agent preserves glucose up to 5 days



Uses: Glucoses, requires full draw (may cause hemolysis if short draw)



LABELING THE SAMPLE - (ANSWER)A properly labeled sample is essential so that the results of the test
match the patient. The key elements in labeling are:



-Patient's surname, first and middle.