Opstellen van betrouwbare drc
LV1216B
2025-03-28
Inleiding
In dit experiment wordt onderzocht hoe er een dosis-responsecurve opgesteld kan worden voor propranolol en metoprolol. De bijbehorende
deelvragen zijn:
Hoe kan een dosis-responscurve voor propranolol worden opgesteld bij een maximale concentratie van 200 µg/mL, gebaseerd op de relatie tussen
concentratie en hartslagfrequentie? en Hoe kan een dosis-responscurve voor metoprolol worden opgesteld bij een maximale concentratie van
3000 µg/mL, gebaseerd op de relatie tussen concentratie en hartslagfrequentie?
methode
Voor dit experiment zijn er per concentratie drie Daphnia’s met elk drie replicaten blootgesteld aan bètablokkers, waarna de hartslag driemaal 10
seconden geteld werd.
data inlezen
ruwe_data_p <- read_excel("watervlo3.xlsx", sheet = 2)
ruwe_data_m <- read_excel("watervlo3.xlsx", sheet = 3)
kable(ruwe_data_p, caption = "Tabel 1: Ruwe data van propranolol")
Tabel 1: Ruwe data van propranolol
daphnia_id propranolol_ugPermL replicate exposure_status beats_per_10_sec
daphnia_200_1 2e+02 replicate_1 pre_exposure 51
daphnia_200_1 2e+02 replicate_2 pre_exposure 52
daphnia_200_1 2e+02 replicate_3 pre_exposure 53
daphnia_200_1 2e+02 replicate_1 post_exposure 11
daphnia_200_1 2e+02 replicate_2 post_exposure 9
daphnia_200_1 2e+02 replicate_3 post_exposure 8
daphnia_200_2 2e+02 replicate_1 pre_exposure 36
daphnia_200_2 2e+02 replicate_2 pre_exposure 36
daphnia_200_2 2e+02 replicate_3 pre_exposure 37
daphnia_200_2 2e+02 replicate_1 post_exposure 5
daphnia_200_2 2e+02 replicate_2 post_exposure 7
daphnia_200_2 2e+02 replicate_3 post_exposure 6
daphnia_200_3 2e+02 replicate_1 pre_exposure 33
daphnia_200_3 2e+02 replicate_2 pre_exposure 31
daphnia_200_3 2e+02 replicate_3 pre_exposure 32
daphnia_200_3 2e+02 replicate_1 post_exposure 15
daphnia_200_3 2e+02 replicate_2 post_exposure 14
daphnia_200_3 2e+02 replicate_3 post_exposure 11
daphnia_20_1 2e+01 replicate_1 pre_exposure 46
daphnia_20_1 2e+01 replicate_2 pre_exposure 48
daphnia_20_1 2e+01 replicate_3 pre_exposure 52
daphnia_20_1 2e+01 replicate_1 post_exposure 10
daphnia_20_1 2e+01 replicate_2 post_exposure 10
,daphnia_20_1 2e+01 replicate_2 post_exposure 10
daphnia_20_1 2e+01 replicate_3 post_exposure 10
daphnia_20_2 2e+01 replicate_1 pre_exposure 23
daphnia_20_2 2e+01 replicate_2 pre_exposure 19
daphnia_20_2 2e+01 replicate_3 pre_exposure 26
daphnia_20_2 2e+01 replicate_1 post_exposure 5
daphnia_20_2 2e+01 replicate_2 post_exposure 5
daphnia_20_2 2e+01 replicate_3 post_exposure 5
daphnia_20_3 2e+01 replicate_1 pre_exposure 40
daphnia_20_3 2e+01 replicate_2 pre_exposure 42
daphnia_20_3 2e+01 replicate_3 pre_exposure 45
daphnia_20_3 2e+01 replicate_1 post_exposure 14
daphnia_20_3 2e+01 replicate_2 post_exposure 14
daphnia_20_3 2e+01 replicate_3 post_exposure 13
daphnia_2_1 2e+00 replicate_1 pre_exposure 49
daphnia_2_1 2e+00 replicate_2 pre_exposure 45
daphnia_2_1 2e+00 replicate_3 pre_exposure 52
daphnia_2_1 2e+00 replicate_1 post_exposure 17
daphnia_2_1 2e+00 replicate_2 post_exposure 10
daphnia_2_1 2e+00 replicate_3 post_exposure 12
daphnia_2_2 2e+00 replicate_1 pre_exposure 56
daphnia_2_2 2e+00 replicate_2 pre_exposure 55
daphnia_2_2 2e+00 replicate_3 pre_exposure 58
daphnia_2_2 2e+00 replicate_1 post_exposure 12
daphnia_2_2 2e+00 replicate_2 post_exposure 12
daphnia_2_2 2e+00 replicate_3 post_exposure 12
daphnia_2_3 2e+00 replicate_1 pre_exposure 40
daphnia_2_3 2e+00 replicate_2 pre_exposure 42
daphnia_2_3 2e+00 replicate_3 pre_exposure 42
daphnia_2_3 2e+00 replicate_1 post_exposure 14
daphnia_2_3 2e+00 replicate_2 post_exposure 20
daphnia_2_3 2e+00 replicate_3 post_exposure 17
daphnia_02_1 2e-01 replicate_1 pre_exposure 49
daphnia_02_1 2e-01 replicate_2 pre_exposure 38
daphnia_02_1 2e-01 replicate_3 pre_exposure 48
daphnia_02_1 2e-01 replicate_1 post_exposure 10
daphnia_02_1 2e-01 replicate_2 post_exposure 9
daphnia_02_1 2e-01 replicate_3 post_exposure 8
daphnia_02_2 2e-01 replicate_1 pre_exposure 31
daphnia_02_2 2e-01 replicate_2 pre_exposure 35
daphnia_02_2 2e-01 replicate_3 pre_exposure 35
daphnia_02_2 2e-01 replicate_1 post_exposure 14
daphnia_02_2 2e-01 replicate_2 post_exposure 13
daphnia_02_2 2e-01 replicate_3 post_exposure 14
daphnia_02_3 2e-01 replicate_1 pre_exposure 37
daphnia_02_3 2e-01 replicate_2 pre_exposure 44
daphnia_02_3 2e-01 replicate_3 pre_exposure 38
,daphnia_02_3 2e-01 replicate_3 pre_exposure 38
daphnia_02_3 2e-01 replicate_1 post_exposure 10
daphnia_02_3 2e-01 replicate_2 post_exposure 11
daphnia_02_3 2e-01 replicate_3 post_exposure 10
daphnia_002_1 2e-02 replicate_1 pre_exposure 47
daphnia_002_1 2e-02 replicate_2 pre_exposure 40
daphnia_002_1 2e-02 replicate_3 pre_exposure 52
daphnia_002_1 2e-02 replicate_1 post_exposure 8
daphnia_002_1 2e-02 replicate_2 post_exposure 6
daphnia_002_1 2e-02 replicate_3 post_exposure 7
daphnia_002_2 2e-02 replicate_1 pre_exposure 32
daphnia_002_2 2e-02 replicate_2 pre_exposure 35
daphnia_002_2 2e-02 replicate_3 pre_exposure 36
daphnia_002_2 2e-02 replicate_1 post_exposure 13
daphnia_002_2 2e-02 replicate_2 post_exposure 14
daphnia_002_2 2e-02 replicate_3 post_exposure 14
daphnia_002_3 2e-02 replicate_1 pre_exposure 43
daphnia_002_3 2e-02 replicate_2 pre_exposure 46
daphnia_002_3 2e-02 replicate_3 pre_exposure 45
daphnia_002_3 2e-02 replicate_1 post_exposure 9
daphnia_002_3 2e-02 replicate_2 post_exposure 7
daphnia_002_3 2e-02 replicate_3 post_exposure 7
In tabel 1 is de ruwe data van het experiment met propranolol weergeven. Hierin is de hartslag per 10 seconden te zien voor elke Daphnia per
concentratie.
kable(ruwe_data_m, caption = "Tabel 2: Ruwe data van metoprolol")
Tabel 2: Ruwe data van metoprolol
daphnia_id metoprolol_ugPermL replicate exposure_status beats_per_10_sec
daphnia_3000_1 3e+03 replicate_1 pre_exposure 49
daphnia_3000_1 3e+03 replicate_2 pre_exposure 52
daphnia_3000_1 3e+03 replicate_3 pre_exposure 50
daphnia_3000_1 3e+03 replicate_1 post_exposure 16
daphnia_3000_1 3e+03 replicate_2 post_exposure 17
daphnia_3000_1 3e+03 replicate_3 post_exposure 16
daphnia_3000_2 3e+03 replicate_1 pre_exposure 61
daphnia_3000_2 3e+03 replicate_2 pre_exposure 62
daphnia_3000_2 3e+03 replicate_3 pre_exposure 57
daphnia_3000_2 3e+03 replicate_1 post_exposure 22
daphnia_3000_2 3e+03 replicate_2 post_exposure 22
daphnia_3000_2 3e+03 replicate_3 post_exposure 22
daphnia_3000_3 3e+03 replicate_1 pre_exposure 45
daphnia_3000_3 3e+03 replicate_2 pre_exposure 44
daphnia_3000_3 3e+03 replicate_3 pre_exposure 45
daphnia_3000_3 3e+03 replicate_1 post_exposure 12
daphnia_3000_3 3e+03 replicate_2 post_exposure 12
daphnia_3000_3 3e+03 replicate_3 post_exposure 12
daphnia_300_1 3e+02 replicate_1 pre_exposure 48
, daphnia_300_1 3e+02 replicate_1 pre_exposure 48
daphnia_300_1 3e+02 replicate_2 pre_exposure 53
daphnia_300_1 3e+02 replicate_3 pre_exposure 48
daphnia_300_1 3e+02 replicate_1 post_exposure 17
daphnia_300_1 3e+02 replicate_2 post_exposure 20
daphnia_300_1 3e+02 replicate_3 post_exposure 21
daphnia_300_2 3e+02 replicate_1 pre_exposure 54
daphnia_300_2 3e+02 replicate_2 pre_exposure 56
daphnia_300_2 3e+02 replicate_3 pre_exposure 54
daphnia_300_2 3e+02 replicate_1 post_exposure 20
daphnia_300_2 3e+02 replicate_2 post_exposure 20
daphnia_300_2 3e+02 replicate_3 post_exposure 20
daphnia_300_3 3e+02 replicate_1 pre_exposure 37
daphnia_300_3 3e+02 replicate_2 pre_exposure 39
daphnia_300_3 3e+02 replicate_3 pre_exposure 38
daphnia_300_3 3e+02 replicate_1 post_exposure 12
daphnia_300_3 3e+02 replicate_2 post_exposure 14
daphnia_300_3 3e+02 replicate_3 post_exposure 15
daphnia_30_1 3e+01 replicate_1 pre_exposure 47
daphnia_30_1 3e+01 replicate_2 pre_exposure 61
daphnia_30_1 3e+01 replicate_3 pre_exposure 51
daphnia_30_1 3e+01 replicate_1 post_exposure 50
daphnia_30_1 3e+01 replicate_2 post_exposure 40
daphnia_30_1 3e+01 replicate_3 post_exposure 36
daphnia_30_2 3e+01 replicate_1 pre_exposure 53
daphnia_30_2 3e+01 replicate_2 pre_exposure 55
daphnia_30_2 3e+01 replicate_3 pre_exposure 58
daphnia_30_2 3e+01 replicate_1 post_exposure 59
daphnia_30_2 3e+01 replicate_2 post_exposure 65
daphnia_30_2 3e+01 replicate_3 post_exposure 54
daphnia_30_3 3e+01 replicate_1 pre_exposure 39
daphnia_30_3 3e+01 replicate_2 pre_exposure 41
daphnia_30_3 3e+01 replicate_3 pre_exposure 40
daphnia_30_3 3e+01 replicate_1 post_exposure 56
daphnia_30_3 3e+01 replicate_2 post_exposure 58
daphnia_30_3 3e+01 replicate_3 post_exposure 56
daphnia_3_1 3e+00 replicate_1 pre_exposure 51
daphnia_3_1 3e+00 replicate_2 pre_exposure 54
daphnia_3_1 3e+00 replicate_3 pre_exposure 52
daphnia_3_1 3e+00 replicate_1 post_exposure 51
daphnia_3_1 3e+00 replicate_2 post_exposure 51
daphnia_3_1 3e+00 replicate_3 post_exposure 61
daphnia_3_2 3e+00 replicate_1 pre_exposure 54
daphnia_3_2 3e+00 replicate_2 pre_exposure 51
daphnia_3_2 3e+00 replicate_3 pre_exposure 60
daphnia_3_2 3e+00 replicate_1 post_exposure 47
daphnia_3_2 3e+00 replicate_2 post_exposure 46
LV1216B
2025-03-28
Inleiding
In dit experiment wordt onderzocht hoe er een dosis-responsecurve opgesteld kan worden voor propranolol en metoprolol. De bijbehorende
deelvragen zijn:
Hoe kan een dosis-responscurve voor propranolol worden opgesteld bij een maximale concentratie van 200 µg/mL, gebaseerd op de relatie tussen
concentratie en hartslagfrequentie? en Hoe kan een dosis-responscurve voor metoprolol worden opgesteld bij een maximale concentratie van
3000 µg/mL, gebaseerd op de relatie tussen concentratie en hartslagfrequentie?
methode
Voor dit experiment zijn er per concentratie drie Daphnia’s met elk drie replicaten blootgesteld aan bètablokkers, waarna de hartslag driemaal 10
seconden geteld werd.
data inlezen
ruwe_data_p <- read_excel("watervlo3.xlsx", sheet = 2)
ruwe_data_m <- read_excel("watervlo3.xlsx", sheet = 3)
kable(ruwe_data_p, caption = "Tabel 1: Ruwe data van propranolol")
Tabel 1: Ruwe data van propranolol
daphnia_id propranolol_ugPermL replicate exposure_status beats_per_10_sec
daphnia_200_1 2e+02 replicate_1 pre_exposure 51
daphnia_200_1 2e+02 replicate_2 pre_exposure 52
daphnia_200_1 2e+02 replicate_3 pre_exposure 53
daphnia_200_1 2e+02 replicate_1 post_exposure 11
daphnia_200_1 2e+02 replicate_2 post_exposure 9
daphnia_200_1 2e+02 replicate_3 post_exposure 8
daphnia_200_2 2e+02 replicate_1 pre_exposure 36
daphnia_200_2 2e+02 replicate_2 pre_exposure 36
daphnia_200_2 2e+02 replicate_3 pre_exposure 37
daphnia_200_2 2e+02 replicate_1 post_exposure 5
daphnia_200_2 2e+02 replicate_2 post_exposure 7
daphnia_200_2 2e+02 replicate_3 post_exposure 6
daphnia_200_3 2e+02 replicate_1 pre_exposure 33
daphnia_200_3 2e+02 replicate_2 pre_exposure 31
daphnia_200_3 2e+02 replicate_3 pre_exposure 32
daphnia_200_3 2e+02 replicate_1 post_exposure 15
daphnia_200_3 2e+02 replicate_2 post_exposure 14
daphnia_200_3 2e+02 replicate_3 post_exposure 11
daphnia_20_1 2e+01 replicate_1 pre_exposure 46
daphnia_20_1 2e+01 replicate_2 pre_exposure 48
daphnia_20_1 2e+01 replicate_3 pre_exposure 52
daphnia_20_1 2e+01 replicate_1 post_exposure 10
daphnia_20_1 2e+01 replicate_2 post_exposure 10
,daphnia_20_1 2e+01 replicate_2 post_exposure 10
daphnia_20_1 2e+01 replicate_3 post_exposure 10
daphnia_20_2 2e+01 replicate_1 pre_exposure 23
daphnia_20_2 2e+01 replicate_2 pre_exposure 19
daphnia_20_2 2e+01 replicate_3 pre_exposure 26
daphnia_20_2 2e+01 replicate_1 post_exposure 5
daphnia_20_2 2e+01 replicate_2 post_exposure 5
daphnia_20_2 2e+01 replicate_3 post_exposure 5
daphnia_20_3 2e+01 replicate_1 pre_exposure 40
daphnia_20_3 2e+01 replicate_2 pre_exposure 42
daphnia_20_3 2e+01 replicate_3 pre_exposure 45
daphnia_20_3 2e+01 replicate_1 post_exposure 14
daphnia_20_3 2e+01 replicate_2 post_exposure 14
daphnia_20_3 2e+01 replicate_3 post_exposure 13
daphnia_2_1 2e+00 replicate_1 pre_exposure 49
daphnia_2_1 2e+00 replicate_2 pre_exposure 45
daphnia_2_1 2e+00 replicate_3 pre_exposure 52
daphnia_2_1 2e+00 replicate_1 post_exposure 17
daphnia_2_1 2e+00 replicate_2 post_exposure 10
daphnia_2_1 2e+00 replicate_3 post_exposure 12
daphnia_2_2 2e+00 replicate_1 pre_exposure 56
daphnia_2_2 2e+00 replicate_2 pre_exposure 55
daphnia_2_2 2e+00 replicate_3 pre_exposure 58
daphnia_2_2 2e+00 replicate_1 post_exposure 12
daphnia_2_2 2e+00 replicate_2 post_exposure 12
daphnia_2_2 2e+00 replicate_3 post_exposure 12
daphnia_2_3 2e+00 replicate_1 pre_exposure 40
daphnia_2_3 2e+00 replicate_2 pre_exposure 42
daphnia_2_3 2e+00 replicate_3 pre_exposure 42
daphnia_2_3 2e+00 replicate_1 post_exposure 14
daphnia_2_3 2e+00 replicate_2 post_exposure 20
daphnia_2_3 2e+00 replicate_3 post_exposure 17
daphnia_02_1 2e-01 replicate_1 pre_exposure 49
daphnia_02_1 2e-01 replicate_2 pre_exposure 38
daphnia_02_1 2e-01 replicate_3 pre_exposure 48
daphnia_02_1 2e-01 replicate_1 post_exposure 10
daphnia_02_1 2e-01 replicate_2 post_exposure 9
daphnia_02_1 2e-01 replicate_3 post_exposure 8
daphnia_02_2 2e-01 replicate_1 pre_exposure 31
daphnia_02_2 2e-01 replicate_2 pre_exposure 35
daphnia_02_2 2e-01 replicate_3 pre_exposure 35
daphnia_02_2 2e-01 replicate_1 post_exposure 14
daphnia_02_2 2e-01 replicate_2 post_exposure 13
daphnia_02_2 2e-01 replicate_3 post_exposure 14
daphnia_02_3 2e-01 replicate_1 pre_exposure 37
daphnia_02_3 2e-01 replicate_2 pre_exposure 44
daphnia_02_3 2e-01 replicate_3 pre_exposure 38
,daphnia_02_3 2e-01 replicate_3 pre_exposure 38
daphnia_02_3 2e-01 replicate_1 post_exposure 10
daphnia_02_3 2e-01 replicate_2 post_exposure 11
daphnia_02_3 2e-01 replicate_3 post_exposure 10
daphnia_002_1 2e-02 replicate_1 pre_exposure 47
daphnia_002_1 2e-02 replicate_2 pre_exposure 40
daphnia_002_1 2e-02 replicate_3 pre_exposure 52
daphnia_002_1 2e-02 replicate_1 post_exposure 8
daphnia_002_1 2e-02 replicate_2 post_exposure 6
daphnia_002_1 2e-02 replicate_3 post_exposure 7
daphnia_002_2 2e-02 replicate_1 pre_exposure 32
daphnia_002_2 2e-02 replicate_2 pre_exposure 35
daphnia_002_2 2e-02 replicate_3 pre_exposure 36
daphnia_002_2 2e-02 replicate_1 post_exposure 13
daphnia_002_2 2e-02 replicate_2 post_exposure 14
daphnia_002_2 2e-02 replicate_3 post_exposure 14
daphnia_002_3 2e-02 replicate_1 pre_exposure 43
daphnia_002_3 2e-02 replicate_2 pre_exposure 46
daphnia_002_3 2e-02 replicate_3 pre_exposure 45
daphnia_002_3 2e-02 replicate_1 post_exposure 9
daphnia_002_3 2e-02 replicate_2 post_exposure 7
daphnia_002_3 2e-02 replicate_3 post_exposure 7
In tabel 1 is de ruwe data van het experiment met propranolol weergeven. Hierin is de hartslag per 10 seconden te zien voor elke Daphnia per
concentratie.
kable(ruwe_data_m, caption = "Tabel 2: Ruwe data van metoprolol")
Tabel 2: Ruwe data van metoprolol
daphnia_id metoprolol_ugPermL replicate exposure_status beats_per_10_sec
daphnia_3000_1 3e+03 replicate_1 pre_exposure 49
daphnia_3000_1 3e+03 replicate_2 pre_exposure 52
daphnia_3000_1 3e+03 replicate_3 pre_exposure 50
daphnia_3000_1 3e+03 replicate_1 post_exposure 16
daphnia_3000_1 3e+03 replicate_2 post_exposure 17
daphnia_3000_1 3e+03 replicate_3 post_exposure 16
daphnia_3000_2 3e+03 replicate_1 pre_exposure 61
daphnia_3000_2 3e+03 replicate_2 pre_exposure 62
daphnia_3000_2 3e+03 replicate_3 pre_exposure 57
daphnia_3000_2 3e+03 replicate_1 post_exposure 22
daphnia_3000_2 3e+03 replicate_2 post_exposure 22
daphnia_3000_2 3e+03 replicate_3 post_exposure 22
daphnia_3000_3 3e+03 replicate_1 pre_exposure 45
daphnia_3000_3 3e+03 replicate_2 pre_exposure 44
daphnia_3000_3 3e+03 replicate_3 pre_exposure 45
daphnia_3000_3 3e+03 replicate_1 post_exposure 12
daphnia_3000_3 3e+03 replicate_2 post_exposure 12
daphnia_3000_3 3e+03 replicate_3 post_exposure 12
daphnia_300_1 3e+02 replicate_1 pre_exposure 48
, daphnia_300_1 3e+02 replicate_1 pre_exposure 48
daphnia_300_1 3e+02 replicate_2 pre_exposure 53
daphnia_300_1 3e+02 replicate_3 pre_exposure 48
daphnia_300_1 3e+02 replicate_1 post_exposure 17
daphnia_300_1 3e+02 replicate_2 post_exposure 20
daphnia_300_1 3e+02 replicate_3 post_exposure 21
daphnia_300_2 3e+02 replicate_1 pre_exposure 54
daphnia_300_2 3e+02 replicate_2 pre_exposure 56
daphnia_300_2 3e+02 replicate_3 pre_exposure 54
daphnia_300_2 3e+02 replicate_1 post_exposure 20
daphnia_300_2 3e+02 replicate_2 post_exposure 20
daphnia_300_2 3e+02 replicate_3 post_exposure 20
daphnia_300_3 3e+02 replicate_1 pre_exposure 37
daphnia_300_3 3e+02 replicate_2 pre_exposure 39
daphnia_300_3 3e+02 replicate_3 pre_exposure 38
daphnia_300_3 3e+02 replicate_1 post_exposure 12
daphnia_300_3 3e+02 replicate_2 post_exposure 14
daphnia_300_3 3e+02 replicate_3 post_exposure 15
daphnia_30_1 3e+01 replicate_1 pre_exposure 47
daphnia_30_1 3e+01 replicate_2 pre_exposure 61
daphnia_30_1 3e+01 replicate_3 pre_exposure 51
daphnia_30_1 3e+01 replicate_1 post_exposure 50
daphnia_30_1 3e+01 replicate_2 post_exposure 40
daphnia_30_1 3e+01 replicate_3 post_exposure 36
daphnia_30_2 3e+01 replicate_1 pre_exposure 53
daphnia_30_2 3e+01 replicate_2 pre_exposure 55
daphnia_30_2 3e+01 replicate_3 pre_exposure 58
daphnia_30_2 3e+01 replicate_1 post_exposure 59
daphnia_30_2 3e+01 replicate_2 post_exposure 65
daphnia_30_2 3e+01 replicate_3 post_exposure 54
daphnia_30_3 3e+01 replicate_1 pre_exposure 39
daphnia_30_3 3e+01 replicate_2 pre_exposure 41
daphnia_30_3 3e+01 replicate_3 pre_exposure 40
daphnia_30_3 3e+01 replicate_1 post_exposure 56
daphnia_30_3 3e+01 replicate_2 post_exposure 58
daphnia_30_3 3e+01 replicate_3 post_exposure 56
daphnia_3_1 3e+00 replicate_1 pre_exposure 51
daphnia_3_1 3e+00 replicate_2 pre_exposure 54
daphnia_3_1 3e+00 replicate_3 pre_exposure 52
daphnia_3_1 3e+00 replicate_1 post_exposure 51
daphnia_3_1 3e+00 replicate_2 post_exposure 51
daphnia_3_1 3e+00 replicate_3 post_exposure 61
daphnia_3_2 3e+00 replicate_1 pre_exposure 54
daphnia_3_2 3e+00 replicate_2 pre_exposure 51
daphnia_3_2 3e+00 replicate_3 pre_exposure 60
daphnia_3_2 3e+00 replicate_1 post_exposure 47
daphnia_3_2 3e+00 replicate_2 post_exposure 46
