CHT EXAM COMPLETE STUDY GUIDE
QUESTIONS WITH 100%VERIFIED
ANSWERS
\.____ a EDTA is preferred over ACD as an additive for molecular testing - Answer- True
\.____ b- EDTA chelates calcium and is acceptable for CDC assays - Answer- False
\.____ c- EDTA is acceptable for flow cytometry assays - Answer- True
\.____ d- Moderate hemolysis of serum should not interfere with PRA testing - Answer- True
\.____ e- Plasma is unacceptable for CDC PRA testing - Answer- True
\.____ f- Heparinized blood is acceptable for molecular testing - Answer- True, MOSTLY
\.____ g- Heparin does not interfere with Taq polymerase - Answer- False
\.____ h- Lithium heparin is an acceptable substitute for sodium heparin - Answer- False
\.____ i- It is acceptable for a red top to be collected from a patient who is at that moment
receiving a dialysis treatment - Answer- False
,\.____ j- Blood shipped to the lab for serology testing should be kept at 4 degrees - Answer-
False
\.____ k- Patients receiving long-term steroid treatment may have insufficient lymphocytes for
HLA testing - Answer- True
\.____ l- Patients receiving blood products should have their blood collected for HLA typing
immediately following the transfusions to ensure adequate cell numbers - Answer- False
\.____ m- You should ask for the WBC and differential on leukopenic and pediatric patients to
determine how much blood should be collected for HLA typing - Answer- True
\.____ n- Requisition forms must include name of an authorized requester - Answer- True
\.____ o- Plain red top, EDTA and ACD are all acceptable samples for ABO blood grouping -
Answer- True
\.____ p- Lymphocytes from EBV transformed cell lines react the same as PBLs in CDC assays -
Answer- False
\.____ q- The color of the stopper is a universal code for a vacutainer's contents - Answer-
False
\.____ r- EDTA blood stored for 1 year at RT is an acceptable sample - Answer- False
\.____ s- Blood spotted on SS 903 cards and stored for 1 year at RT is an acceptable sample -
Answer- True
,\.____ t- ACD tubes maintain cell viability are better than heparin during long transit times -
Answer- True
\._____ also inspect laboratories, and because of its deemed status with other regulatory
agencies (OSHA, HCFA, UNOS, JCAHO), It is compelled to notify those agencies when an
incidence occur. - Answer- ASHI
\.______ has the mandate to control pollution of environment - Answer- EPA
\._______ are the major receptor cluster expressed on human NK cells and subset
of T cells. - Answer- KIRs
\.________ is an ultra-sensitive fluorescent dye that undergoes a dramatic fluorescence
enhancement upon binding to dsDNA that can be measured using a microplate fluorometer.
Simpler and more sensitive (ability to detect 1ng/mL of dsDNA) compared to Hoechst 33258
Dye. - Answer- PicoGreen
\._________ are typically
cold reacting (4oC) and________are typically warm reacting (22-37oC). - Answer-
Autoantibodies
B cell antibodies
\._________ would be helpful in determining the volume of blood needed for testing. - Answer-
WBC count
\.__________ are increasingly recognized as an important immune
component involved in immune surveillance, anti-infection, as well as alloimmune
, response. - Answer- Natural killer (NK) cells
\.___________ must be chosen so that the markers are flanking
on each side the expected DNA migration. They are used to determine migration outcome by
comparing the patient specimen migration with the known marker migration. - Answer- The
DNA ladder
\._______________ is a fluorescent dye that intercalates between the
bases of DNA and RNA. - Answer- Ethidium bromide
\."MISSING SELF" ON A CANCEROUS OR INFECTED CELL PERMITS ______ ACTIVATION - Answer-
NK
\.(ASHI) - Answer- American Society of Histocompatibility and Immunogenetics
\.(FBS) - Answer- Fetal Bovine Serum can be a suitable substitute for PHS. However, FBS may
be cytotoxic to human cells and, therefore, must be tested in CDC assays prior to use in typing
trays.
\.(IHWG) - Answer- With International Histocompatibility Workshop Group
\.(PHS) - Answer- Pooled human sera from non-immunized, blood group
AB male donors. PHS provides an additional protein source that is essential in maintaining the
integrity of cell
membranes during cell growth, storage or any subsequent testing.
\.(SEOPF) - Answer- Southeastern Organ Procurement Foundation
QUESTIONS WITH 100%VERIFIED
ANSWERS
\.____ a EDTA is preferred over ACD as an additive for molecular testing - Answer- True
\.____ b- EDTA chelates calcium and is acceptable for CDC assays - Answer- False
\.____ c- EDTA is acceptable for flow cytometry assays - Answer- True
\.____ d- Moderate hemolysis of serum should not interfere with PRA testing - Answer- True
\.____ e- Plasma is unacceptable for CDC PRA testing - Answer- True
\.____ f- Heparinized blood is acceptable for molecular testing - Answer- True, MOSTLY
\.____ g- Heparin does not interfere with Taq polymerase - Answer- False
\.____ h- Lithium heparin is an acceptable substitute for sodium heparin - Answer- False
\.____ i- It is acceptable for a red top to be collected from a patient who is at that moment
receiving a dialysis treatment - Answer- False
,\.____ j- Blood shipped to the lab for serology testing should be kept at 4 degrees - Answer-
False
\.____ k- Patients receiving long-term steroid treatment may have insufficient lymphocytes for
HLA testing - Answer- True
\.____ l- Patients receiving blood products should have their blood collected for HLA typing
immediately following the transfusions to ensure adequate cell numbers - Answer- False
\.____ m- You should ask for the WBC and differential on leukopenic and pediatric patients to
determine how much blood should be collected for HLA typing - Answer- True
\.____ n- Requisition forms must include name of an authorized requester - Answer- True
\.____ o- Plain red top, EDTA and ACD are all acceptable samples for ABO blood grouping -
Answer- True
\.____ p- Lymphocytes from EBV transformed cell lines react the same as PBLs in CDC assays -
Answer- False
\.____ q- The color of the stopper is a universal code for a vacutainer's contents - Answer-
False
\.____ r- EDTA blood stored for 1 year at RT is an acceptable sample - Answer- False
\.____ s- Blood spotted on SS 903 cards and stored for 1 year at RT is an acceptable sample -
Answer- True
,\.____ t- ACD tubes maintain cell viability are better than heparin during long transit times -
Answer- True
\._____ also inspect laboratories, and because of its deemed status with other regulatory
agencies (OSHA, HCFA, UNOS, JCAHO), It is compelled to notify those agencies when an
incidence occur. - Answer- ASHI
\.______ has the mandate to control pollution of environment - Answer- EPA
\._______ are the major receptor cluster expressed on human NK cells and subset
of T cells. - Answer- KIRs
\.________ is an ultra-sensitive fluorescent dye that undergoes a dramatic fluorescence
enhancement upon binding to dsDNA that can be measured using a microplate fluorometer.
Simpler and more sensitive (ability to detect 1ng/mL of dsDNA) compared to Hoechst 33258
Dye. - Answer- PicoGreen
\._________ are typically
cold reacting (4oC) and________are typically warm reacting (22-37oC). - Answer-
Autoantibodies
B cell antibodies
\._________ would be helpful in determining the volume of blood needed for testing. - Answer-
WBC count
\.__________ are increasingly recognized as an important immune
component involved in immune surveillance, anti-infection, as well as alloimmune
, response. - Answer- Natural killer (NK) cells
\.___________ must be chosen so that the markers are flanking
on each side the expected DNA migration. They are used to determine migration outcome by
comparing the patient specimen migration with the known marker migration. - Answer- The
DNA ladder
\._______________ is a fluorescent dye that intercalates between the
bases of DNA and RNA. - Answer- Ethidium bromide
\."MISSING SELF" ON A CANCEROUS OR INFECTED CELL PERMITS ______ ACTIVATION - Answer-
NK
\.(ASHI) - Answer- American Society of Histocompatibility and Immunogenetics
\.(FBS) - Answer- Fetal Bovine Serum can be a suitable substitute for PHS. However, FBS may
be cytotoxic to human cells and, therefore, must be tested in CDC assays prior to use in typing
trays.
\.(IHWG) - Answer- With International Histocompatibility Workshop Group
\.(PHS) - Answer- Pooled human sera from non-immunized, blood group
AB male donors. PHS provides an additional protein source that is essential in maintaining the
integrity of cell
membranes during cell growth, storage or any subsequent testing.
\.(SEOPF) - Answer- Southeastern Organ Procurement Foundation
