FC assignments;
Nummer; 1
Homo sapiens taste 2 receptor member 1 (TAS2R1), transcript variant 1, mRNA
Homo sapiens taste 2 receptor member 1 (TAS2R1), transcript variant 1, - Nucleotide - NCBI (nih.gov)
translation="MLESHLIIYFLLAVIQFLLGIFTNGIIVVVNGIDLIKHRKMAPL
DLLLSCLAVSRIFLQLFIFYVNVIVIFFIEFIMCSANCAILLFINELELWLATWLGVF
YCAKVASVRHPLFIWLKMRISKLVPWMILGSLLYVSMICVFHSKYAGFMVPYFLRKFF
SQNATIQKEDTLAIQIFSFVAEFSVPLLIFLFAVLLLIFSLGRHTRQMRNTVAGSRVP
GRGAPISALLSILSFLILYFSHCMIKVFLSSLKFHIRRFIFLFFILVIGIYPSGHSLI
LILGNPKLKQNAKKFLLHSKCCQ"
NCBI Blast:Protein Sequence (nih.gov)
Best match = gorilla gorilla gorilla
, ProtScale analysis (expasy.org)
➔ 6 trans memraan helices ipv 7? Twee helices samengeperst?
Forward primer: CCTTCGTTTTCTTGGTGAATTTTTGGGATGTAGTGAAGAGGCGG
Reverse primer; GGTTGGCTTGGTTTGCAATCATCC
Best match;
Homo sapiens taste 2 receptor member 38 (TAS2R38), mRNA
https://www.ncbi.nlm.nih.gov/nuccore/NM_176817.5
Match forward primer;
Nummer; 1
Homo sapiens taste 2 receptor member 1 (TAS2R1), transcript variant 1, mRNA
Homo sapiens taste 2 receptor member 1 (TAS2R1), transcript variant 1, - Nucleotide - NCBI (nih.gov)
translation="MLESHLIIYFLLAVIQFLLGIFTNGIIVVVNGIDLIKHRKMAPL
DLLLSCLAVSRIFLQLFIFYVNVIVIFFIEFIMCSANCAILLFINELELWLATWLGVF
YCAKVASVRHPLFIWLKMRISKLVPWMILGSLLYVSMICVFHSKYAGFMVPYFLRKFF
SQNATIQKEDTLAIQIFSFVAEFSVPLLIFLFAVLLLIFSLGRHTRQMRNTVAGSRVP
GRGAPISALLSILSFLILYFSHCMIKVFLSSLKFHIRRFIFLFFILVIGIYPSGHSLI
LILGNPKLKQNAKKFLLHSKCCQ"
NCBI Blast:Protein Sequence (nih.gov)
Best match = gorilla gorilla gorilla
, ProtScale analysis (expasy.org)
➔ 6 trans memraan helices ipv 7? Twee helices samengeperst?
Forward primer: CCTTCGTTTTCTTGGTGAATTTTTGGGATGTAGTGAAGAGGCGG
Reverse primer; GGTTGGCTTGGTTTGCAATCATCC
Best match;
Homo sapiens taste 2 receptor member 38 (TAS2R38), mRNA
https://www.ncbi.nlm.nih.gov/nuccore/NM_176817.5
Match forward primer;
