Inhoud
1 Celbiologie Eiwitten en translocatie in het ER deel 1 ........................................................................... 3
1.1 Eiwitsynthese, aminozuren en post translationele modificaties .................................................. 3
1.2 Vouwing: spontaan en m.b.v. chaperon eiwitten ......................................................................... 4
1.3 Afbraak eiwitten ............................................................................................................................ 5
1.4 Conformatie verandering door allosterische liganden ................................................................. 8
1.5 Celorganellen................................................................................................................................. 9
1.6 Transport van eiwitten uit cytoplasma naar ER .......................................................................... 10
1.7 Postranslationele modificaties in ER ........................................................................................... 12
2 Celbiologie Eiwitten en translocatie in het ER deel 2 ......................................................................... 13
2.1 Unfolded protein response (UPR) ............................................................................................... 13
2.2 Lipide bilaag gevormd door ER .................................................................................................... 15
2.3 Nucleaire import en export van moleculen ................................................................................ 16
2.4 Transport van eiwitten naar mitochondria ................................................................................. 17
2.5 Peroxisomen ................................................................................................................................ 18
3 Celbiologie Vesiculair transport ......................................................................................................... 19
3.1 Introductie vesiculair transport ................................................................................................... 19
3.2 Clathrine coated vesicles ............................................................................................................. 19
3.3 Rol van phosphoinisotides........................................................................................................... 20
3.4 COP coated vesicles ..................................................................................................................... 21
3.5 Rol van Rabs en SNARES .............................................................................................................. 22
3.6 Transport tussen ER en Golgi ..................................................................................................... 22
3.7 Modificatie en sortering in het Golgi .......................................................................................... 23
3.8 Lysosomen ................................................................................................................................... 23
3.9 Transport naar lysosomen ........................................................................................................... 24
3.10 Endocytose ................................................................................................................................ 25
3.11 Exocytose................................................................................................................................... 26
4 Celbiologie Cel communicatie deel 1 ................................................................................................. 27
4.1 Algemene principes van cel communicatie ................................................................................. 27
4.2 Intracellulaire receptoren............................................................................................................ 28
4.3 G-eiwit gekoppelde receptoren (GPCR) ...................................................................................... 28
4.4 Adrenaline signalering via GPCR, cAMP en PKA .......................................................................... 29
5 Celbiologie Cel communicatie deel 2 ................................................................................................. 32
5.1 GPCR signalering via fosfolipase C-β ........................................................................................... 32
1 Celbiologie Eiwitten en translocatie in het ER deel 1 ........................................................................... 3
1.1 Eiwitsynthese, aminozuren en post translationele modificaties .................................................. 3
1.2 Vouwing: spontaan en m.b.v. chaperon eiwitten ......................................................................... 4
1.3 Afbraak eiwitten ............................................................................................................................ 5
1.4 Conformatie verandering door allosterische liganden ................................................................. 8
1.5 Celorganellen................................................................................................................................. 9
1.6 Transport van eiwitten uit cytoplasma naar ER .......................................................................... 10
1.7 Postranslationele modificaties in ER ........................................................................................... 12
2 Celbiologie Eiwitten en translocatie in het ER deel 2 ......................................................................... 13
2.1 Unfolded protein response (UPR) ............................................................................................... 13
2.2 Lipide bilaag gevormd door ER .................................................................................................... 15
2.3 Nucleaire import en export van moleculen ................................................................................ 16
2.4 Transport van eiwitten naar mitochondria ................................................................................. 17
2.5 Peroxisomen ................................................................................................................................ 18
3 Celbiologie Vesiculair transport ......................................................................................................... 19
3.1 Introductie vesiculair transport ................................................................................................... 19
3.2 Clathrine coated vesicles ............................................................................................................. 19
3.3 Rol van phosphoinisotides........................................................................................................... 20
3.4 COP coated vesicles ..................................................................................................................... 21
3.5 Rol van Rabs en SNARES .............................................................................................................. 22
3.6 Transport tussen ER en Golgi ..................................................................................................... 22
3.7 Modificatie en sortering in het Golgi .......................................................................................... 23
3.8 Lysosomen ................................................................................................................................... 23
3.9 Transport naar lysosomen ........................................................................................................... 24
3.10 Endocytose ................................................................................................................................ 25
3.11 Exocytose................................................................................................................................... 26
4 Celbiologie Cel communicatie deel 1 ................................................................................................. 27
4.1 Algemene principes van cel communicatie ................................................................................. 27
4.2 Intracellulaire receptoren............................................................................................................ 28
4.3 G-eiwit gekoppelde receptoren (GPCR) ...................................................................................... 28
4.4 Adrenaline signalering via GPCR, cAMP en PKA .......................................................................... 29
5 Celbiologie Cel communicatie deel 2 ................................................................................................. 32
5.1 GPCR signalering via fosfolipase C-β ........................................................................................... 32
