Unit 2c: Practical Scientific Procedures and Techniques
Issue date: 11/03/2022
Deadline: 24/03/2022
Introduction:
For this assignment we had to complete two chromatography practicals;
Firstly, we had to conduct a paper chromatography to separate and identify the different colours
inside the different felt tip pens we used.
And secondly, we had to conduct thin layer chromatography, in which we needed to do to identify
the different components in the mixture of paracetamol.
This assignment will be a report on how we conducted these experiments, the method we used and
the results that we obtained.
Paper Chromatography of felt tip pens
Equipment:
A selection of at least five different coloured felt tip pens.
A glass rod or pencil
Water (Solvent)
Chromatography paper
250ml beaker
Tape
Ruler
Risk assessment:
The only real risk in this practical would be the risk of breaking and cutting yourself on the glassware
used. In which case, the safety precautions would be to handle carefully and not leaving the
glassware anywhere near the edge where it could fall and break- and if it breaks don’t pick it up with
your hands, you should clean it up with a dustpan and brush. If you are cut, seek medical attention.
There are a few other general safety precautions that you should take like wearing a lab coat, safety
goggles, gloves and if you have long hair then having your hair tied back.
Method:
The method that we were required to follow is described below:
Firstly, we drew a line with a pencil straight across the chromatography paper, about 1cm from the
bottom, we then picked four different felt tip pens and marked four different points with each of
them, equally spread across the paper, and labelled them as A, B, C, D.
, We then attached the chromatography paper to a glass rod with tape and placed it into the beaker
and started adding water until it was about 0.5cm up.
After waiting for the water to move through the paper, we removed the chromatography paper just
as it reached the top and allowed the chromatogram to dry.
This was our result:
Issue date: 11/03/2022
Deadline: 24/03/2022
Introduction:
For this assignment we had to complete two chromatography practicals;
Firstly, we had to conduct a paper chromatography to separate and identify the different colours
inside the different felt tip pens we used.
And secondly, we had to conduct thin layer chromatography, in which we needed to do to identify
the different components in the mixture of paracetamol.
This assignment will be a report on how we conducted these experiments, the method we used and
the results that we obtained.
Paper Chromatography of felt tip pens
Equipment:
A selection of at least five different coloured felt tip pens.
A glass rod or pencil
Water (Solvent)
Chromatography paper
250ml beaker
Tape
Ruler
Risk assessment:
The only real risk in this practical would be the risk of breaking and cutting yourself on the glassware
used. In which case, the safety precautions would be to handle carefully and not leaving the
glassware anywhere near the edge where it could fall and break- and if it breaks don’t pick it up with
your hands, you should clean it up with a dustpan and brush. If you are cut, seek medical attention.
There are a few other general safety precautions that you should take like wearing a lab coat, safety
goggles, gloves and if you have long hair then having your hair tied back.
Method:
The method that we were required to follow is described below:
Firstly, we drew a line with a pencil straight across the chromatography paper, about 1cm from the
bottom, we then picked four different felt tip pens and marked four different points with each of
them, equally spread across the paper, and labelled them as A, B, C, D.
, We then attached the chromatography paper to a glass rod with tape and placed it into the beaker
and started adding water until it was about 0.5cm up.
After waiting for the water to move through the paper, we removed the chromatography paper just
as it reached the top and allowed the chromatogram to dry.
This was our result:
