Turcan SBB exam review AABB Packet
questions & answers 2023/2024
Rh inheritence of Rhnull - ANSWER-Lack LW
Lack FY5
KEL3 Issues - ANSWER-KPa
in cis position may have suppressive effect on other Kell system antigens
Adsorption applications in RBC testing - ANSWER-Remove autoantibody
Separate multiple antibodies
Confirm antigen or antibody specificity
Antilymphocyte Globulin (ALG) - ANSWER-Made from horse serum
Interferes with DAT and IAT
Relation to Lu(a-b-)
PCR and TMA - ANSWER-PCR used for DNA amp
TMA used for RNA amp
Autologous donor qualifications - ANSWER-DR's order
Hgb >11, HCT 33%
Collected >72 hrs before surgery or Tx
Deferred if risk of bacteremia
Plateletpheresis count info - ANSWER->150,000 min
pre procedure plt count not required for infrequent donors
,post-procedure plt count may be used to qualify donor for next procedure
Two units RBC apheresis post donation H/H - ANSWER-Donor HCT not <30% or Hgb <10 g/dL after
volume replacement following donation
Anticoagulants and purpose of - ANSWER-Dextrose:
Supports ATP generation
Adenine:
Provides substrate for ATP synthesis
Sodium biphosphate:
Controls pH or is buffer
Citrate:
prevents clotting and coagulation
Changes in bag upon storage - ANSWER-decreases in:
pH
2,3-DPG
ATP
Increases in:
Hgb
K
Oxygen dissociation curve information - ANSWER-p50 is pO2 level at which Hgb is 50% saturated
A normal p50 is about 25 pO2
increase p50, see right shift, more O2 released
decrease p50, see left shift, less O2 released
Reactive test results for TTD action - ANSWER-Reactive results must be retested in duplicate
, Confirmatory TTD tests - ANSWER-Neutralization for HBsAg and HIV-1 Ag
WB for Anti-HIV
RIBA for Anti-HCV
Anti-HTLV confirmation
No algorithm available for reentry by FDA for these - ANSWER-HIV and HCV NAT pos tests
Components for correction of coagulopathies - ANSWER-Plasma
PLTs
Cryo
Components for replacing deficient coag factors - ANSWER-FFP
Cryo
Components for fighting infections - ANSWER-WBCs
Use for cryo-poor plasma - ANSWER-treat TTP patients
PLTs purpose and contraindication - ANSWER-Improve hemostasis
do not give for TTP or ITP unless absolutely necessary
Fathers genotype is BO, mom's is OO. Prob to have 3 OO kids? - ANSWER-0.5x0.5x0.5 = .125
Calcium's involvement in coag cascade - ANSWER-involved at:
12a and 11 reaction to 11a
11a and 9 reaction to 9a
9a's and 8a's reaction on 10 to 10a
10a's and 5a's reaction on prothrombin to thrombin
questions & answers 2023/2024
Rh inheritence of Rhnull - ANSWER-Lack LW
Lack FY5
KEL3 Issues - ANSWER-KPa
in cis position may have suppressive effect on other Kell system antigens
Adsorption applications in RBC testing - ANSWER-Remove autoantibody
Separate multiple antibodies
Confirm antigen or antibody specificity
Antilymphocyte Globulin (ALG) - ANSWER-Made from horse serum
Interferes with DAT and IAT
Relation to Lu(a-b-)
PCR and TMA - ANSWER-PCR used for DNA amp
TMA used for RNA amp
Autologous donor qualifications - ANSWER-DR's order
Hgb >11, HCT 33%
Collected >72 hrs before surgery or Tx
Deferred if risk of bacteremia
Plateletpheresis count info - ANSWER->150,000 min
pre procedure plt count not required for infrequent donors
,post-procedure plt count may be used to qualify donor for next procedure
Two units RBC apheresis post donation H/H - ANSWER-Donor HCT not <30% or Hgb <10 g/dL after
volume replacement following donation
Anticoagulants and purpose of - ANSWER-Dextrose:
Supports ATP generation
Adenine:
Provides substrate for ATP synthesis
Sodium biphosphate:
Controls pH or is buffer
Citrate:
prevents clotting and coagulation
Changes in bag upon storage - ANSWER-decreases in:
pH
2,3-DPG
ATP
Increases in:
Hgb
K
Oxygen dissociation curve information - ANSWER-p50 is pO2 level at which Hgb is 50% saturated
A normal p50 is about 25 pO2
increase p50, see right shift, more O2 released
decrease p50, see left shift, less O2 released
Reactive test results for TTD action - ANSWER-Reactive results must be retested in duplicate
, Confirmatory TTD tests - ANSWER-Neutralization for HBsAg and HIV-1 Ag
WB for Anti-HIV
RIBA for Anti-HCV
Anti-HTLV confirmation
No algorithm available for reentry by FDA for these - ANSWER-HIV and HCV NAT pos tests
Components for correction of coagulopathies - ANSWER-Plasma
PLTs
Cryo
Components for replacing deficient coag factors - ANSWER-FFP
Cryo
Components for fighting infections - ANSWER-WBCs
Use for cryo-poor plasma - ANSWER-treat TTP patients
PLTs purpose and contraindication - ANSWER-Improve hemostasis
do not give for TTP or ITP unless absolutely necessary
Fathers genotype is BO, mom's is OO. Prob to have 3 OO kids? - ANSWER-0.5x0.5x0.5 = .125
Calcium's involvement in coag cascade - ANSWER-involved at:
12a and 11 reaction to 11a
11a and 9 reaction to 9a
9a's and 8a's reaction on 10 to 10a
10a's and 5a's reaction on prothrombin to thrombin
