Flow Cytometry/Fluorescence-Activated Cell
Sorting (FACS) Questions with Verified
Correct Answers
flow cytometry:
a way of identifying cells, using antibodies, based on the molecule they express
interrogation:
cells are in liqiuid stream and as the cells o single file by the laser, the laser analyzes each cell
Fluorochromes:
fluorescent dyes attached to antibody
T cells: CD3+
CD4+, CD8+, CD4-8-
Nk cells: Ig-CD3-
CD16+, CD5+
b cells: Ig+
Ig+, CD5+
how flow cytometry works:
-antibody bound to cell marker on surface of the cell and antibody has fluorochrome
-when laser hits fluorochrome, the dye absorbs NRG from light
-the dye gets excited and gives off certain color of light
emission color of R-phycoerythrin (PE):
orange
Sorting (FACS) Questions with Verified
Correct Answers
flow cytometry:
a way of identifying cells, using antibodies, based on the molecule they express
interrogation:
cells are in liqiuid stream and as the cells o single file by the laser, the laser analyzes each cell
Fluorochromes:
fluorescent dyes attached to antibody
T cells: CD3+
CD4+, CD8+, CD4-8-
Nk cells: Ig-CD3-
CD16+, CD5+
b cells: Ig+
Ig+, CD5+
how flow cytometry works:
-antibody bound to cell marker on surface of the cell and antibody has fluorochrome
-when laser hits fluorochrome, the dye absorbs NRG from light
-the dye gets excited and gives off certain color of light
emission color of R-phycoerythrin (PE):
orange
