BIOD 171 Final Exam Review (150+ Questions) Portage
Learning 2026 | Real Exam Questions & Verified Answers
| Complete Prep
1. How would you define "contrast"?
the amount of light that can be taken in by the lens
a point at which an object is enlarged much that no further information
can be obtained
the distance between 2 objects at which you can still see them as
distinct
none of the above
the difference between the object and its background
2. A group of elementary students became ill after eating undercooked ground
beef prepared in the school cafeteria. E. coli serotype O157:H7 is the
suspected pathogen. Which of the following types of media should be used
to confirm the identification of this pathogen?
MacConkey agar with sorbitol
Hektoen-Enteric agar
MacConkey agar
XLD agar
3. In the streak-plate method, streaking the bacteria from region to region on
the Petri dish serves the purpose of
increasing the number of bacteria you will have for further
experiments.
decreasing the numbers of bacteria in each area, producing
, individual colonies in the final area.
spreading the bacteria around the plate more evenly.
limiting the number of bacteria that may grow on the plate.
4. Describe the significance of fluorescence in studying protein expression in
cells.
Fluorescence indicates the overall health of the cell.
Fluorescence allows researchers to visualize and quantify the
expression levels of specific proteins within cells.
Fluorescence is used to measure the temperature of the cell.
Fluorescence helps in determining the DNA sequence of the cell.
5. Describe the significance of the Protista kingdom in the context of cellular
structure and classification.
The Protista kingdom consists only of multicellular organisms that are
essential for plant growth.
The Protista kingdom is exclusively made up of bacteria and viruses.
The Protista kingdom includes primarily unicellular organisms that
play crucial roles in ecosystems and serve as a bridge between
prokaryotic and multicellular life forms.
The Protista kingdom includes only organisms that are harmful to
humans.
6. When inoculating a sterile petri dish from an environment, what technique is
commonly used and what is the hoped for result of the technique when
identifying possible bacteria species.
Streaking the plate to produce individual isolated colonies.
Transferring the environment to your fingers and then spreading the
, environment on to the petri dish.
Making a lawn from the environment of choice to get a uniform
coverage of the petri dish.
Waving the plate over the environment and hoping spores from the
environment will transfer to the petri dish.
7. Which of the following technique(s) are capable of producing a three-
dimensional representation of a biological sample:
electron microscopy tomography
scanning electron microscopy
serial optical sectioning by confocal microscopy followed by image
reconstruction.
none of the above.
all of the above
8. What are the two primary functions of glucose-6-phosphate in cellular
metabolism?
DNA replication and RNA transcription
Energy production and glycogen synthesis
Protein synthesis and lipid metabolism
Cell signaling and membrane formation
9. Which of the following is the most correct interpretation of the figure?
, Energy from catabolism can be used directly for performing cellular
work.
ATP is a molecule that acts as an intermediary to store chemical
energy for cellular work.
Pi acts as a shuttle molecule to move energy from ATP to ADP.
ADP + Pi are a set of molecules that store energy for catabolism.
10. GFP would be used in which of the following types of microscopes?
phase contrast microscope
fluorescent microscope
differential interference contrast (DIC) microscope
electron microscope
atomic force microscope
11. Describe the role of MacConkey agar in the detection of E. coli O157:H7.
MacConkey agar inhibits the growth of gram-negative bacteria.
MacConkey agar promotes the growth of all bacteria equally.
MacConkey agar differentiates lactose fermenters from non-
fermenters, allowing for the identification of E. coli O157:H7.
MacConkey agar is used primarily for culturing fungi.
12. Small phase shifts in the light passing through a transparent specimen are
converted into amplitude or contrast changes in the image
Learning 2026 | Real Exam Questions & Verified Answers
| Complete Prep
1. How would you define "contrast"?
the amount of light that can be taken in by the lens
a point at which an object is enlarged much that no further information
can be obtained
the distance between 2 objects at which you can still see them as
distinct
none of the above
the difference between the object and its background
2. A group of elementary students became ill after eating undercooked ground
beef prepared in the school cafeteria. E. coli serotype O157:H7 is the
suspected pathogen. Which of the following types of media should be used
to confirm the identification of this pathogen?
MacConkey agar with sorbitol
Hektoen-Enteric agar
MacConkey agar
XLD agar
3. In the streak-plate method, streaking the bacteria from region to region on
the Petri dish serves the purpose of
increasing the number of bacteria you will have for further
experiments.
decreasing the numbers of bacteria in each area, producing
, individual colonies in the final area.
spreading the bacteria around the plate more evenly.
limiting the number of bacteria that may grow on the plate.
4. Describe the significance of fluorescence in studying protein expression in
cells.
Fluorescence indicates the overall health of the cell.
Fluorescence allows researchers to visualize and quantify the
expression levels of specific proteins within cells.
Fluorescence is used to measure the temperature of the cell.
Fluorescence helps in determining the DNA sequence of the cell.
5. Describe the significance of the Protista kingdom in the context of cellular
structure and classification.
The Protista kingdom consists only of multicellular organisms that are
essential for plant growth.
The Protista kingdom is exclusively made up of bacteria and viruses.
The Protista kingdom includes primarily unicellular organisms that
play crucial roles in ecosystems and serve as a bridge between
prokaryotic and multicellular life forms.
The Protista kingdom includes only organisms that are harmful to
humans.
6. When inoculating a sterile petri dish from an environment, what technique is
commonly used and what is the hoped for result of the technique when
identifying possible bacteria species.
Streaking the plate to produce individual isolated colonies.
Transferring the environment to your fingers and then spreading the
, environment on to the petri dish.
Making a lawn from the environment of choice to get a uniform
coverage of the petri dish.
Waving the plate over the environment and hoping spores from the
environment will transfer to the petri dish.
7. Which of the following technique(s) are capable of producing a three-
dimensional representation of a biological sample:
electron microscopy tomography
scanning electron microscopy
serial optical sectioning by confocal microscopy followed by image
reconstruction.
none of the above.
all of the above
8. What are the two primary functions of glucose-6-phosphate in cellular
metabolism?
DNA replication and RNA transcription
Energy production and glycogen synthesis
Protein synthesis and lipid metabolism
Cell signaling and membrane formation
9. Which of the following is the most correct interpretation of the figure?
, Energy from catabolism can be used directly for performing cellular
work.
ATP is a molecule that acts as an intermediary to store chemical
energy for cellular work.
Pi acts as a shuttle molecule to move energy from ATP to ADP.
ADP + Pi are a set of molecules that store energy for catabolism.
10. GFP would be used in which of the following types of microscopes?
phase contrast microscope
fluorescent microscope
differential interference contrast (DIC) microscope
electron microscope
atomic force microscope
11. Describe the role of MacConkey agar in the detection of E. coli O157:H7.
MacConkey agar inhibits the growth of gram-negative bacteria.
MacConkey agar promotes the growth of all bacteria equally.
MacConkey agar differentiates lactose fermenters from non-
fermenters, allowing for the identification of E. coli O157:H7.
MacConkey agar is used primarily for culturing fungi.
12. Small phase shifts in the light passing through a transparent specimen are
converted into amplitude or contrast changes in the image
