LAB PRACTICAL 2 BIOL: Muscle Tissue Anatomy & Physiology Questions with Correct Answers & Detailed Rationales 2026/2027 Edition

LAB PRACTICAL 2 BIOL: Muscle Tissue Anatomy &
Physiology Questions with Correct Answers & Detailed
Rationales 2026/2027 Edition

SECTION 1: MUSCLE TISSUE MEMBRANES & COVERINGS
(Questions 1–20)


Question 1: Identify the membrane in muscle tissue that surrounds an individual
muscle fascicle.
• A) Endomysium
• B) Perimysium
• C) Epimysium
• D) Sarcolemma
Answer: B
Rationale: Perimysium is the connective tissue membrane that surrounds each
fascicle (bundle of muscle fibers). The endomysium surrounds individual muscle
fibers, and the epimysium surrounds the entire muscle.


Question 2: The outer connective tissue covering that surrounds the entire skeletal
muscle is called the:
• A) Perimysium
• B) Endomysium
• C) Epimysium
• D) Sarcolemma
Answer: C

,Rationale: Epimysium is the outer connective tissue covering that surrounds the
entire skeletal muscle. It is continuous with the tendons that attach the muscle to
bone.


Question 3: The connective tissue membrane that surrounds individual muscle
fibers is called the:
• A) Perimysium
• B) Endomysium
• C) Epimysium
• D) Sarcoplasmic reticulum
Answer: B
Rationale: Endomysium is the thin connective tissue membrane that surrounds
each individual muscle fiber (cell). It provides support and contains capillaries and
nerve endings.


Question 4: The plasma membrane of an individual muscle fiber is called the:
• A) Endomysium
• B) Perimysium
• C) Epimysium
• D) Sarcolemma
Answer: D
Rationale: The sarcolemma is the plasma membrane of a muscle fiber. It
surrounds the sarcoplasm (cytoplasm) and is responsible for conducting action
potentials.


Question 5: The dark striations in skeletal muscle tissue are called:
• A) A bands
• B) I bands

, • C) H zones
• D) M lines
Answer: A
Rationale: A bands are the dark striations in muscle tissue. They contain the thick
filaments (myosin) and are anisotropic (appear dark under polarized light).


Question 6: The light striations in skeletal muscle tissue are called:
• A) A bands
• B) I bands
• C) H zones
• D) Z discs
Answer: B
Rationale: I bands are the light striations in muscle tissue. They contain only thin
filaments (actin) and are isotropic (appear light under polarized light).


Question 7: The region of the sarcomere where only myosin filaments are present
is the:
• A) I band
• B) Z disc
• C) M line
• D) H zone
Answer: D
Rationale: The H zone is the central region of the sarcomere where only myosin
(thick) filaments are present. It shortens during contraction.


Question 8: The structure that anchors thin filaments in the sarcomere is the:
• A) M line

, • B) Z disc
• C) H zone
• D) A band
Answer: B
Rationale: The Z disc anchors the thin filaments (actin) and marks the boundary of
each sarcomere. It appears as a dark line in the middle of the I band.


Question 9: The structure in the center of the sarcomere that anchors thick
filaments is the:
• A) Z disc
• B) M line
• C) I band
• D) H zone
Answer: B
Rationale: The M line is in the center of the sarcomere and anchors the thick
filaments (myosin). It contains proteins that hold the myosin filaments in
alignment.


Question 10: A sarcomere extends from:
• A) M line to M line
• B) Z disc to Z disc
• C) A band to A band
• D) I band to I band
Answer: B
Rationale: A sarcomere extends from one Z disc to the next Z disc. It is the basic
contractile unit of skeletal muscle.