Biol 2444 Micro LAB Final Exam Latest Update
undefined media-1 - ANSWER exact ingredient concentrations unknown
defined media-1 - ANSWER all ingredients are purified and precisely known
when added to prepare the media
sterilization-1 - ANSWER process that eliminates or deactivates all biological
agents (bacteria, fungi, viruses, etc.)
autoclave settings-1 - ANSWER 121 degree Celcius and 15 PSI
3 phases of autoclave cycle-1 - ANSWER purge, sterilization, exhaust
purge phase-1 - ANSWER steam flows into the chamber and replaces air with
steam, pressure is ramped up slowly
sterilizaton phase-1 - ANSWER exhaust valve is closed causing the temperature
and pressure to reach the desired setpoint
exhaust phase-1 - ANSWER pressure is slowly released from the chamber
through an exhaust valve until ambient pressure is reached
pathogenic relationship-1 - ANSWER harmful to host
communalistic relationship-1 - ANSWER neutral to host
mutualistic relationship-1 - ANSWER beneficial to host
microbiocidal-1 - ANSWER kill microbes
microbiostatic-1 - ANSWER inhibit microbe growth
condenser-1 - ANSWER creates a condensed light of beam
objective lens-1 - ANSWER The lens on a light microscope that is closest to the
stage. 4-100X
ocular lens-1 - ANSWER the lens on a light microscope that your eyes look
through. 10X
compound microscope-1 - ANSWER a microscope that uses 2 or more lenses
, total magnification-1 - ANSWER objective lens x ocular lens
resolution-1 - ANSWER clarity of the specimen image, goes down with higher
levels of magnification
numerical aperture-1 - ANSWER measure of a lens ability to "capture" light
coming from the specimen and use it to make the image
immersion oil-1 - ANSWER substance that can be used on the higher power
objective lenses such as 100X to aid in capturing light
course knob-1 - ANSWER only used on 4X and 10X
fine knob-1 - ANSWER only used on 40X and 100X
binary fission-2 - ANSWER divides exponentially until nutrients are depleted
colony forming unit-2 - ANSWER cluster of cells visible to the naked eye
3 categories for describing colonies-2 - ANSWER form, elevation, margin
streak plate isolation technique-2 - ANSWER diluting the polymicrobial mixture
until a single bacterial cell or CFU can be obtained, T-streak and Quad-streak
are commonly used
basic stains-3 - ANSWER stains that have a positive charge and attracted to
negatively charged cell walls, include methylene blue, crystal violet, safranin
acidic stains-3 - ANSWER stains that have a negative charge and attracted to
positively charged cell walls, include eosin, India ink, acid fuschin
simple staining-3 - ANSWER the use of a single basic stain
differential staining-3 - ANSWER the use of more than one stain
bacterial smear-3 - ANSWER bacterial emulsion is added to a clean slide and
then gently heated over a flame to allow fixation
primary stain in gram stain-3 - ANSWER crystal violet
mordant in gram stain-3 - ANSWER iodine
counterstain in gram stain-3 - ANSWER safranin
undefined media-1 - ANSWER exact ingredient concentrations unknown
defined media-1 - ANSWER all ingredients are purified and precisely known
when added to prepare the media
sterilization-1 - ANSWER process that eliminates or deactivates all biological
agents (bacteria, fungi, viruses, etc.)
autoclave settings-1 - ANSWER 121 degree Celcius and 15 PSI
3 phases of autoclave cycle-1 - ANSWER purge, sterilization, exhaust
purge phase-1 - ANSWER steam flows into the chamber and replaces air with
steam, pressure is ramped up slowly
sterilizaton phase-1 - ANSWER exhaust valve is closed causing the temperature
and pressure to reach the desired setpoint
exhaust phase-1 - ANSWER pressure is slowly released from the chamber
through an exhaust valve until ambient pressure is reached
pathogenic relationship-1 - ANSWER harmful to host
communalistic relationship-1 - ANSWER neutral to host
mutualistic relationship-1 - ANSWER beneficial to host
microbiocidal-1 - ANSWER kill microbes
microbiostatic-1 - ANSWER inhibit microbe growth
condenser-1 - ANSWER creates a condensed light of beam
objective lens-1 - ANSWER The lens on a light microscope that is closest to the
stage. 4-100X
ocular lens-1 - ANSWER the lens on a light microscope that your eyes look
through. 10X
compound microscope-1 - ANSWER a microscope that uses 2 or more lenses
, total magnification-1 - ANSWER objective lens x ocular lens
resolution-1 - ANSWER clarity of the specimen image, goes down with higher
levels of magnification
numerical aperture-1 - ANSWER measure of a lens ability to "capture" light
coming from the specimen and use it to make the image
immersion oil-1 - ANSWER substance that can be used on the higher power
objective lenses such as 100X to aid in capturing light
course knob-1 - ANSWER only used on 4X and 10X
fine knob-1 - ANSWER only used on 40X and 100X
binary fission-2 - ANSWER divides exponentially until nutrients are depleted
colony forming unit-2 - ANSWER cluster of cells visible to the naked eye
3 categories for describing colonies-2 - ANSWER form, elevation, margin
streak plate isolation technique-2 - ANSWER diluting the polymicrobial mixture
until a single bacterial cell or CFU can be obtained, T-streak and Quad-streak
are commonly used
basic stains-3 - ANSWER stains that have a positive charge and attracted to
negatively charged cell walls, include methylene blue, crystal violet, safranin
acidic stains-3 - ANSWER stains that have a negative charge and attracted to
positively charged cell walls, include eosin, India ink, acid fuschin
simple staining-3 - ANSWER the use of a single basic stain
differential staining-3 - ANSWER the use of more than one stain
bacterial smear-3 - ANSWER bacterial emulsion is added to a clean slide and
then gently heated over a flame to allow fixation
primary stain in gram stain-3 - ANSWER crystal violet
mordant in gram stain-3 - ANSWER iodine
counterstain in gram stain-3 - ANSWER safranin
