MGG EXAM 4 QUESTIONS AND ANSWERS

MGG EXAM 4 QUESTIONS AND ANSWERS
A FASTA file always begins with a:

a. <

b. @

c. /

d. > - CORRECT ANSWER✅✅D



Besides the > sign, the first line of a FASTA file includes:

a. the read quality data

b. the sequence

c. the accession number for the sequence

d. the type of sequencing equipment used in obtaining the sequence - CORRECT ANSWER✅✅C



The second line of a FASTA file comprises:

a. the sequence

b. the read quality data

c. the accession number for the sequence

d. the type of sequencing equipment used in obtaining the sequence - CORRECT ANSWER✅✅A



A FASTQ file always begins with a:

a. <

b. >

c. @

d. / - CORRECT ANSWER✅✅C



The second line of a FASTQ file comprises:

a.the read quality data

b. the accession number for the sequence

, c. the sequence

d. the type of sequencing equipment used in obtaining the sequence - CORRECT ANSWER✅✅C



The fourth line of FASTQ line comprises:

a. the accession number for the sequence

b. the type of sequencing equipment used in obtaining the sequence

c. the read quality data

d. the sequence - CORRECT ANSWER✅✅C



A PHRED score of 40 for a particular base call corresponds to the probability of 1 base call error in how
many base calls? [Note: this is a Review Quiz question; you won't be asked to memorize the formula for
the exam]

a. 40

b. 4,000

c. 10,000

d. 40,000 - CORRECT ANSWER✅✅C



What does a PHRED score of 30 or above indicate?

a. Low error rate for the entire read; acceptable quality

b. Low error rate for that base call; acceptable quality

c. High error rate for that base call; unacceptable quality

d. High error rate for the entire read; unacceptable quality - CORRECT ANSWER✅✅B



Which symbol represents a PHRED score of 36 in the Sanger FASTQ system for using ASCII symbols to
represent PHRED scores? [Note: this is a Review Quiz question; you won't be asked to memorize the
table in the exam]

a. $

b. E

c. @

d. m - CORRECT ANSWER✅✅B