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BIOL 2444 Lab Final Study Guide Questions
with Correct Answers | Updated (100%
Correct Answers)
level of biosafety Answer: determined by the infectivity, severity of
disease, transmissibility, and nature of work conducted with
microbes in the lab
BSL-2 Lab Contents Answer: - personal protective equipment
including gloves, lab coat, and eye protection
- sinks and eye wash stations
- restricted access to the lab for authorized persons only
- autoclaves or alternative methods of decontamination
Required PPE Answer: gloves, lab apron/coat, goggles, close-toed
shoes, and long pants/skirt
agar Answer: a mixture of polysaccharide and pectin that is derived
from red algae
complex/undefined media Answer: exact ingredient concentrations
are unknown
defined media Answer: ingredients are purified and concentrations
are precisely known; does not contain animal or yeast extract
© 2025 All rights reserved
, 2
sterilization Answer: a process that eliminates or deactivates all
biological agents
parts of an autoclave Answer: - chamber: where items are loaded
- jacket: the area surrounding the chamber
phases of an autoclave cycle Answer: 1. purge phase
2. sterilization phase
3. exhaust phase
purge phase Answer: steam flows into the chamber and replaces air
with steam; pressure is ramped up slowly
sterilization phase Answer: the exhaust valve is closed, causing the
temperature and pressure to reach the desired setpoint; the
autoclave program maintains these settings for the desired time set
exhaust phase Answer: pressure is slowly released from the
chamber through an exhaust valve until ambient pressure is reached
petri dish storage Answer: petri dishes should be stored upside
down to prevent condensation from dripping onto the agar and
spreading bacteria
© 2025 All rights reserved
BIOL 2444 Lab Final Study Guide Questions
with Correct Answers | Updated (100%
Correct Answers)
level of biosafety Answer: determined by the infectivity, severity of
disease, transmissibility, and nature of work conducted with
microbes in the lab
BSL-2 Lab Contents Answer: - personal protective equipment
including gloves, lab coat, and eye protection
- sinks and eye wash stations
- restricted access to the lab for authorized persons only
- autoclaves or alternative methods of decontamination
Required PPE Answer: gloves, lab apron/coat, goggles, close-toed
shoes, and long pants/skirt
agar Answer: a mixture of polysaccharide and pectin that is derived
from red algae
complex/undefined media Answer: exact ingredient concentrations
are unknown
defined media Answer: ingredients are purified and concentrations
are precisely known; does not contain animal or yeast extract
© 2025 All rights reserved
, 2
sterilization Answer: a process that eliminates or deactivates all
biological agents
parts of an autoclave Answer: - chamber: where items are loaded
- jacket: the area surrounding the chamber
phases of an autoclave cycle Answer: 1. purge phase
2. sterilization phase
3. exhaust phase
purge phase Answer: steam flows into the chamber and replaces air
with steam; pressure is ramped up slowly
sterilization phase Answer: the exhaust valve is closed, causing the
temperature and pressure to reach the desired setpoint; the
autoclave program maintains these settings for the desired time set
exhaust phase Answer: pressure is slowly released from the
chamber through an exhaust valve until ambient pressure is reached
petri dish storage Answer: petri dishes should be stored upside
down to prevent condensation from dripping onto the agar and
spreading bacteria
© 2025 All rights reserved
