The manipulation of genes to make useful products
Dates back thousands of years (selective breeding)
Today’s technology is used to modify DNA
Nucleic Acid Extraction
Detergent, the enzymes to destroy proteins, and ethanol remove DNA or RNA
Polymerase Chain Reaction (PCR)
Uses polymerase enzymes to amplify (copy) specific DNA regions
Common uses: DNA sequencing for…
Determining paternity
Genetic diseases
Forensics
Gel Electrophoresis
Separates chromosomes or DNA fragments based on size
DNA has a negative charge
Smaller fragments move faster toward the positive charge
Blotting - Transfer fragments onto nylon “membrane”
DNA Profiling and Forensic Science
Analysis of DNA samples to determine the DNA source
The chance of two people having identical DNA is one in 10 billion to one in several
trillion
Reproductive Cloning of Animals
a. Agricultural uses (dairy cows)
Dates back thousands of years (selective breeding)
Today’s technology is used to modify DNA
Nucleic Acid Extraction
Detergent, the enzymes to destroy proteins, and ethanol remove DNA or RNA
Polymerase Chain Reaction (PCR)
Uses polymerase enzymes to amplify (copy) specific DNA regions
Common uses: DNA sequencing for…
Determining paternity
Genetic diseases
Forensics
Gel Electrophoresis
Separates chromosomes or DNA fragments based on size
DNA has a negative charge
Smaller fragments move faster toward the positive charge
Blotting - Transfer fragments onto nylon “membrane”
DNA Profiling and Forensic Science
Analysis of DNA samples to determine the DNA source
The chance of two people having identical DNA is one in 10 billion to one in several
trillion
Reproductive Cloning of Animals
a. Agricultural uses (dairy cows)
