Practical report in vivo Biomedical Imaging - Ba3 BMW
2024-2025
Name Van Nimmen Femke
Instructions
The demo-practicals in vivo biomedical imaging (MRI, US, PET/SPECT, RX, CT, US,
intervention vascularBLI) give you insight into the different imaging techniques and the
clinical and preclinical (laboratory animals) application.
The mandatory practical report includes assignments from some of the different demonstrated
in vivo imaging techniques: MRI (preclinical and clinical); US; PET/SPECT/CT (virtual) and
BLI imaging.
In the 5 sub-reports (MRI (preclinical and clinical); US; PET/SPECT/CT; BLI), bundled in
one document, you formulate an answer to the questions asked. These may include, for
example, the description of the imaging equipment/scanners used; the principles of the
imaging equipment/scanners; the time course of the recordings + chronological description of
animal handling, etc.
Try to be complete but concise. Ask questions during the demonstrations!
Clearly state your name at the top and save the completed document with an appropriate
name:
“in vivo BMB report 2024_First name_Name.docx”
This document must be submitted via Blackboard no later than December 7th, 2024. Reports
submitted via email will not be assessed.
, Assignment preclinical MRI (max 2.5 pages)
The report is a description of the preclinical MR practical based on the acquired MRI.
The questions below definitely need to answered.
1. Briefly describe the chronological course of the MRI images and animal handling
procedures.
1ste: place the animal in an induction box. In the box is oxygen combined with isoflurane =
the anaesthetic.
Second: Once the rat/mouse's eye reflexes have disappeared we can assume that the animal is
fully anesthetized. Next, we put eye gels the eyes of the animal to protected them from drying
out. Then
we take the animal out of the box put it on the movable table of the MRI with a mask on
his head with anaesthetic (isoflurane) + a rectal probe is inserted in the bud of the animal with
Vaseline, to monitoring the animal’s body temperature. On his foot is pulse oximeter for
tracking his/here’s pulse. (paws of the rat/mice are too small for the pulse oximeter). Brain
monitoring is followed by a path on the head of the rat to detect his brain activity and pressed
firmly to minimize interference from the RF pulses with the skull and hair.
Good the know: the animal must be stable, so he has ‘tied up’ with earplugs and his front
teeth into a small slot. Just to make sure the animal won’t move, and we can take clear
images.
2. Describe the scanners used (preclinical), RF coils and subject.
An indication which scientists use to identify animals includes the following: the small MRI;
specifically conceived for imaging small mammals-like mice or rats, or rabbits; a preclinical
MRI-Imagers known which are specific for the sort of medical experiments one has to
conduct; very high field strength from 7T to 14T for state-of-the-art imaging as well as small
anatomical structures, brain or tumors included; and many made to move closer to small
animals with a total volume excluded of normally-being larger than other MRI devices.
The most important part of an MRI is the coil used for transmission and reception. This coil
varies- volume coils cover large areas as in the entire body imaging, or pass a gradient
imaging for small, targeted areas such, as the brain of the mice. In general, there are coils
made of combinations of other coils that are called phased-array coils, with smaller elements
working together. The coil is usually placed as close to the animal as possible for improved
signal strength.
Preclinical MRI is a powerful, non-invasive tool for researchers concerned with analysing the
development of a disease and its treatment. MRI allows for real-time monitoring of conditions
such as tumours or neurological disorders without the need for invasive procedures.
3. What is a T2-weighted MRI image? Describe the acquisition sequence and parameters for
a T2-weighted MRI.
A T2-weighted MRI is an MRI sequence that is extreme sensitive to the longitudinal
relaxation time (=T2) of tissues. It gives detailed information about the tissue’s water content
and the structure of tissues, so it is efficiently for evaluation of the soft tissues and detecting
abnormalities like oedemas.
2024-2025
Name Van Nimmen Femke
Instructions
The demo-practicals in vivo biomedical imaging (MRI, US, PET/SPECT, RX, CT, US,
intervention vascularBLI) give you insight into the different imaging techniques and the
clinical and preclinical (laboratory animals) application.
The mandatory practical report includes assignments from some of the different demonstrated
in vivo imaging techniques: MRI (preclinical and clinical); US; PET/SPECT/CT (virtual) and
BLI imaging.
In the 5 sub-reports (MRI (preclinical and clinical); US; PET/SPECT/CT; BLI), bundled in
one document, you formulate an answer to the questions asked. These may include, for
example, the description of the imaging equipment/scanners used; the principles of the
imaging equipment/scanners; the time course of the recordings + chronological description of
animal handling, etc.
Try to be complete but concise. Ask questions during the demonstrations!
Clearly state your name at the top and save the completed document with an appropriate
name:
“in vivo BMB report 2024_First name_Name.docx”
This document must be submitted via Blackboard no later than December 7th, 2024. Reports
submitted via email will not be assessed.
, Assignment preclinical MRI (max 2.5 pages)
The report is a description of the preclinical MR practical based on the acquired MRI.
The questions below definitely need to answered.
1. Briefly describe the chronological course of the MRI images and animal handling
procedures.
1ste: place the animal in an induction box. In the box is oxygen combined with isoflurane =
the anaesthetic.
Second: Once the rat/mouse's eye reflexes have disappeared we can assume that the animal is
fully anesthetized. Next, we put eye gels the eyes of the animal to protected them from drying
out. Then
we take the animal out of the box put it on the movable table of the MRI with a mask on
his head with anaesthetic (isoflurane) + a rectal probe is inserted in the bud of the animal with
Vaseline, to monitoring the animal’s body temperature. On his foot is pulse oximeter for
tracking his/here’s pulse. (paws of the rat/mice are too small for the pulse oximeter). Brain
monitoring is followed by a path on the head of the rat to detect his brain activity and pressed
firmly to minimize interference from the RF pulses with the skull and hair.
Good the know: the animal must be stable, so he has ‘tied up’ with earplugs and his front
teeth into a small slot. Just to make sure the animal won’t move, and we can take clear
images.
2. Describe the scanners used (preclinical), RF coils and subject.
An indication which scientists use to identify animals includes the following: the small MRI;
specifically conceived for imaging small mammals-like mice or rats, or rabbits; a preclinical
MRI-Imagers known which are specific for the sort of medical experiments one has to
conduct; very high field strength from 7T to 14T for state-of-the-art imaging as well as small
anatomical structures, brain or tumors included; and many made to move closer to small
animals with a total volume excluded of normally-being larger than other MRI devices.
The most important part of an MRI is the coil used for transmission and reception. This coil
varies- volume coils cover large areas as in the entire body imaging, or pass a gradient
imaging for small, targeted areas such, as the brain of the mice. In general, there are coils
made of combinations of other coils that are called phased-array coils, with smaller elements
working together. The coil is usually placed as close to the animal as possible for improved
signal strength.
Preclinical MRI is a powerful, non-invasive tool for researchers concerned with analysing the
development of a disease and its treatment. MRI allows for real-time monitoring of conditions
such as tumours or neurological disorders without the need for invasive procedures.
3. What is a T2-weighted MRI image? Describe the acquisition sequence and parameters for
a T2-weighted MRI.
A T2-weighted MRI is an MRI sequence that is extreme sensitive to the longitudinal
relaxation time (=T2) of tissues. It gives detailed information about the tissue’s water content
and the structure of tissues, so it is efficiently for evaluation of the soft tissues and detecting
abnormalities like oedemas.
