33.1 CHROMAT0GRAPHY
Chromatography is a tomy ot seporotion techniques that aeperds on elotve
solubibtu in moble phase ond ottinity tor stat1onary phase
- nobile phase
solvent oten colled eluent in column civommatogrophy)
stationwy phase stays still
Thin-Layu chromatogrphy
Mobe phase - solvent
Station ay phase - slica (s,0,) or alumina (Al, 0,) mounted on glass plate
Deparation occurs as the conponents an ether be dissolv ed in solvent
Cr adsorbed On to silca
S:0 ond Alp3 are polar
see spots on
chromatogram using wlght or spray wth a
locating ogent.
R+ volue : dstance nmoved by sput
distance moved by solvernt
Compae Kt value to compute database ot substana R values
’hemicaly Sinular substances wil have very simila Rt values
R+ vaues change depending on the solvent.
Column chromatography
Mobe phase passes through a column contany the
Suitable organi sclvent nd silIca stationary phase
Comon ents move at dteeent rates and can be collected
amount ot time tor seprate
substanu tomove through retntin time
deteior used to detet separatin ot wlouless
substancen
Gos- liqud chramatouraphy
Mobie phase - inet arrie qaS leg N, Ar)
Sotionory phase- iquid odsorbed on inet sol1d support
Somple n eted into the machine and corned oalong th1n
tjme taken to trvel aown clumn column
(retentiom time) detected
s
Substaus identfed buy ompriy retentiun times to database
o area wnde u pealk tels you the
amout of a Sutstane
cas chro,natoyruphy nss spetrumetry'
gas chromotography atiach ed directy to mcss spetrojneter
moss ad troqmentetion putl nca he used to identitu
Chromatography is a tomy ot seporotion techniques that aeperds on elotve
solubibtu in moble phase ond ottinity tor stat1onary phase
- nobile phase
solvent oten colled eluent in column civommatogrophy)
stationwy phase stays still
Thin-Layu chromatogrphy
Mobe phase - solvent
Station ay phase - slica (s,0,) or alumina (Al, 0,) mounted on glass plate
Deparation occurs as the conponents an ether be dissolv ed in solvent
Cr adsorbed On to silca
S:0 ond Alp3 are polar
see spots on
chromatogram using wlght or spray wth a
locating ogent.
R+ volue : dstance nmoved by sput
distance moved by solvernt
Compae Kt value to compute database ot substana R values
’hemicaly Sinular substances wil have very simila Rt values
R+ vaues change depending on the solvent.
Column chromatography
Mobe phase passes through a column contany the
Suitable organi sclvent nd silIca stationary phase
Comon ents move at dteeent rates and can be collected
amount ot time tor seprate
substanu tomove through retntin time
deteior used to detet separatin ot wlouless
substancen
Gos- liqud chramatouraphy
Mobie phase - inet arrie qaS leg N, Ar)
Sotionory phase- iquid odsorbed on inet sol1d support
Somple n eted into the machine and corned oalong th1n
tjme taken to trvel aown clumn column
(retentiom time) detected
s
Substaus identfed buy ompriy retentiun times to database
o area wnde u pealk tels you the
amout of a Sutstane
cas chro,natoyruphy nss spetrumetry'
gas chromotography atiach ed directy to mcss spetrojneter
moss ad troqmentetion putl nca he used to identitu
