Sample Question Paper 2023-24
Class XII
Biotechnology (Subject Code-045)
Maximum Marks: 70 Time: 3 hours
General Instructions:
(i) The question paper has five Sections. All questions are compulsory.
(ii) Section–A contains 12 Multiple choice questions and 4 Assertion-Reasoning based questions of 1
mark each; Section–B has 5 short answer questions of 2 marks each; Section –C has 7 short
answer questions of 3 marks each; Section-D has 2 case based question of 4 marks; Section-E
has three long answer questions of 5 marks each.
(iii) There is no overall choice. However, internal choices have been provided in some questions. A
student has to attempt only one of the alternatives in such questions.
Section - A
Q. No. Question Marks
1 Type II Restriction Enzymes are employed and not type I and type III, in 1
Recombinant DNA technology because :
(a) They recognise a palindromic recognition sequence.
(b) They lyse specifically within the restriction site.
(c) They cleave 1 bp away from the 5’ end.
(d) They cleave both the DNA strands simultaneously.
2 A bull has trouble walking and getting up, He is nervous and violent. This condition 1
is getting worse with time. The causative agent responsible for these symptoms
could be :
(a) Bacillus anthracis
(b) Virion
(c) Mycobacterium bovis
(d) Prion
3 Turbidity measurement method for ascertaining the number of microbial cells in a 1
liquid culture is not accurate. Choose the correct explanation.
(a) It measures both live and dead cells.
(b) It measures low amount of cells too.
(c) It is difficult and non sensitive technique.
(d) It destroys the sample solution.
1
, 4 To increase the efficiency of picking of insert by a vector, Dr. Kumar decided to 1
make use of ___________________ in the genetic engineering protocol. Identify
the tool.
(a) DNA Ligase
(b) DNA Phosphatase
(c) Alkaline Phosphatase
(d) DNA Polymerase
5 Example of Single Cell Protein 1
(a) Yeast
(b) Bacteria
(c) Algae
(d) All of these
6 The first human cell line established by George Gay in 1950s from cervix cancer is 1
called ____________
(a) CHO cell line
(b) Cos-1 cell line
(c) HeLa cell line
(d) Fibroblast cell line
7 In 1603, Baricelli reported that a spectrum of diseases like jaundice, skin lesions etc 1
can be treated by administration of :
(a) Curd
(b) Honey
(c) Milk
(d) Whey
8 An important plant secondary metabolite produced through tissue culture which 1
has found use in antifertility drugs is _________________
(a) Berberine
(b) Quinine
(c) Shikonin
(d) Diosgenin
9 One of the important purpose of Functional Proteomics is : 1
(a) To obtain 3D structure of all the proteins
(b) To identify protein networks in nuclear pore complex
(c) To characterise all protein-protein interactions
(d) To identify disease specific proteins
2
Class XII
Biotechnology (Subject Code-045)
Maximum Marks: 70 Time: 3 hours
General Instructions:
(i) The question paper has five Sections. All questions are compulsory.
(ii) Section–A contains 12 Multiple choice questions and 4 Assertion-Reasoning based questions of 1
mark each; Section–B has 5 short answer questions of 2 marks each; Section –C has 7 short
answer questions of 3 marks each; Section-D has 2 case based question of 4 marks; Section-E
has three long answer questions of 5 marks each.
(iii) There is no overall choice. However, internal choices have been provided in some questions. A
student has to attempt only one of the alternatives in such questions.
Section - A
Q. No. Question Marks
1 Type II Restriction Enzymes are employed and not type I and type III, in 1
Recombinant DNA technology because :
(a) They recognise a palindromic recognition sequence.
(b) They lyse specifically within the restriction site.
(c) They cleave 1 bp away from the 5’ end.
(d) They cleave both the DNA strands simultaneously.
2 A bull has trouble walking and getting up, He is nervous and violent. This condition 1
is getting worse with time. The causative agent responsible for these symptoms
could be :
(a) Bacillus anthracis
(b) Virion
(c) Mycobacterium bovis
(d) Prion
3 Turbidity measurement method for ascertaining the number of microbial cells in a 1
liquid culture is not accurate. Choose the correct explanation.
(a) It measures both live and dead cells.
(b) It measures low amount of cells too.
(c) It is difficult and non sensitive technique.
(d) It destroys the sample solution.
1
, 4 To increase the efficiency of picking of insert by a vector, Dr. Kumar decided to 1
make use of ___________________ in the genetic engineering protocol. Identify
the tool.
(a) DNA Ligase
(b) DNA Phosphatase
(c) Alkaline Phosphatase
(d) DNA Polymerase
5 Example of Single Cell Protein 1
(a) Yeast
(b) Bacteria
(c) Algae
(d) All of these
6 The first human cell line established by George Gay in 1950s from cervix cancer is 1
called ____________
(a) CHO cell line
(b) Cos-1 cell line
(c) HeLa cell line
(d) Fibroblast cell line
7 In 1603, Baricelli reported that a spectrum of diseases like jaundice, skin lesions etc 1
can be treated by administration of :
(a) Curd
(b) Honey
(c) Milk
(d) Whey
8 An important plant secondary metabolite produced through tissue culture which 1
has found use in antifertility drugs is _________________
(a) Berberine
(b) Quinine
(c) Shikonin
(d) Diosgenin
9 One of the important purpose of Functional Proteomics is : 1
(a) To obtain 3D structure of all the proteins
(b) To identify protein networks in nuclear pore complex
(c) To characterise all protein-protein interactions
(d) To identify disease specific proteins
2
