MIC 102 FINAL QUESTIONS & ANSWERS
How would you describe a bacterium that has many flagella originating at a single pole
of the cell?
A. axial filament
B. lophotrichous
c. monotrichous
d. pertrichous
e. perpendicular filament - Answers -B. lophotrichous
Some "microbial" species like Epulopiscium fishelsoni can grow large enough to be
seen by the naked eye. What does a cell typically have to do to be able to handle the
most critical problem of growing to such a large size?
a. creates a series of internal gas vesicles to increase buoyancy
b. creates a smooth surface to decrease the cell's surface area
c. creates folds in the cell membrane to increase the cell's surface area.
d. cover cell in a layer of crystalline layer of protein to provide rigidity. - Answers -c.
creates folds in the cell membrane to increase the cell's surface area.
Bacterial Topoisomerase can facilitate which of the following activities. - Answers -
converting a negatively supercoiled chromosome to the relaxed state
converting a positively supercoiled chromosome into the relaxed state
DNA synthesis on the lagging strand: - Answers --Requires DNA ligase enzyme activity
to complete.
-requires multiple instances of primase activity as it progresses
If you wanted to track the localization of the FtsZ protein in living cells, which type of
microscopy would you use? - Answers -fluorescence
Which type of macromolecule makes up most of a cell's mass and requires most of the
cell's energy to synthesize? - Answers -Protein
Which of the following types of photosynthesis would be considered non-cyclic? -
Answers -- the electron donor is H2O
-the electron donor is a reduced sulfur compound like H2S
Lipopolysaccharide is found: - Answers -in the outer membrane of gram-negative
bacteria
LUCA probably had all of the following properties except: - Answers -a cell wall
containing peptidoglycan
, In order to assimilate sulfur from the environment into cell material - Answers -sulfur
must be reduced prior to assimilation
A transport type that phosphorylates the transported substrate as it enters the cell is: -
Answers -group translocation
The bacterial pilus - Answers --can be used to attach to surfaces
-changes length dynamically by polymerization and depolymerization
-is assembled primarily out of a single protein monomer
Bacteria and Archaea share many features of their cell envelopes, but which feature
can only be found among the Archaea? - Answers -a cell membrane that is a lipid
monolayer
Which of the following is true of the average bacterial nucleoid? - Answers -There is a
single circular chromosome
Initiating DNA replication requires a sufficient supply of ATP and the accumulation of
this protein - Answers -DnaA
You are working with a gut bacterium that forms enterosomes. What do you expect to
happen if you give this bacterium an antibiotic compound that pokes holes in the
enterosomes? - Answers -The cell will no longer be able to safely metabolize
compounds like propanediol
Why does a chemoautotroph sometimes need to run its electron transport chain
backwards? - Answers -To generate NADH which is not made when the transport chain
is run forwards
You are studying the growth of E. coli and for one experiment you decide to use M9
medium. Composition fully listed. After experimentation, you determine that E.coli can
grow in this exact medium, but it cannot grow if any component is missing or added.
What can you say about this medium? - Answers --M9 is a defined growth medium
-M9 is a minimal growth medium for E.coli
Glucose fermentation by some microbes can result in the accumulation of acids such as
acetate and lactate in the medium. The accumulation of these acid byproducts is often
toxic to the cell that makes them, but they do it anyways because fermentation is
necessary to: - Answers -continue ATP production by substrate-level phosphorylation
An organism that has proteins constructed to withstand denaturing, increased amounts
of DNA-binding proteins, and an increased activity of reverse gyrase to form positive
supercoils in the chromosome is probably well adapted to grow in what type of
environment. - Answers -high temperature
How would you describe a bacterium that has many flagella originating at a single pole
of the cell?
A. axial filament
B. lophotrichous
c. monotrichous
d. pertrichous
e. perpendicular filament - Answers -B. lophotrichous
Some "microbial" species like Epulopiscium fishelsoni can grow large enough to be
seen by the naked eye. What does a cell typically have to do to be able to handle the
most critical problem of growing to such a large size?
a. creates a series of internal gas vesicles to increase buoyancy
b. creates a smooth surface to decrease the cell's surface area
c. creates folds in the cell membrane to increase the cell's surface area.
d. cover cell in a layer of crystalline layer of protein to provide rigidity. - Answers -c.
creates folds in the cell membrane to increase the cell's surface area.
Bacterial Topoisomerase can facilitate which of the following activities. - Answers -
converting a negatively supercoiled chromosome to the relaxed state
converting a positively supercoiled chromosome into the relaxed state
DNA synthesis on the lagging strand: - Answers --Requires DNA ligase enzyme activity
to complete.
-requires multiple instances of primase activity as it progresses
If you wanted to track the localization of the FtsZ protein in living cells, which type of
microscopy would you use? - Answers -fluorescence
Which type of macromolecule makes up most of a cell's mass and requires most of the
cell's energy to synthesize? - Answers -Protein
Which of the following types of photosynthesis would be considered non-cyclic? -
Answers -- the electron donor is H2O
-the electron donor is a reduced sulfur compound like H2S
Lipopolysaccharide is found: - Answers -in the outer membrane of gram-negative
bacteria
LUCA probably had all of the following properties except: - Answers -a cell wall
containing peptidoglycan
, In order to assimilate sulfur from the environment into cell material - Answers -sulfur
must be reduced prior to assimilation
A transport type that phosphorylates the transported substrate as it enters the cell is: -
Answers -group translocation
The bacterial pilus - Answers --can be used to attach to surfaces
-changes length dynamically by polymerization and depolymerization
-is assembled primarily out of a single protein monomer
Bacteria and Archaea share many features of their cell envelopes, but which feature
can only be found among the Archaea? - Answers -a cell membrane that is a lipid
monolayer
Which of the following is true of the average bacterial nucleoid? - Answers -There is a
single circular chromosome
Initiating DNA replication requires a sufficient supply of ATP and the accumulation of
this protein - Answers -DnaA
You are working with a gut bacterium that forms enterosomes. What do you expect to
happen if you give this bacterium an antibiotic compound that pokes holes in the
enterosomes? - Answers -The cell will no longer be able to safely metabolize
compounds like propanediol
Why does a chemoautotroph sometimes need to run its electron transport chain
backwards? - Answers -To generate NADH which is not made when the transport chain
is run forwards
You are studying the growth of E. coli and for one experiment you decide to use M9
medium. Composition fully listed. After experimentation, you determine that E.coli can
grow in this exact medium, but it cannot grow if any component is missing or added.
What can you say about this medium? - Answers --M9 is a defined growth medium
-M9 is a minimal growth medium for E.coli
Glucose fermentation by some microbes can result in the accumulation of acids such as
acetate and lactate in the medium. The accumulation of these acid byproducts is often
toxic to the cell that makes them, but they do it anyways because fermentation is
necessary to: - Answers -continue ATP production by substrate-level phosphorylation
An organism that has proteins constructed to withstand denaturing, increased amounts
of DNA-binding proteins, and an increased activity of reverse gyrase to form positive
supercoils in the chromosome is probably well adapted to grow in what type of
environment. - Answers -high temperature
