Nested pcr Guides d'étude, Notes de cours & Résumés

MB (ASCP) 2020(updated) correctly answeredConsider the table below where a child and a possible father (PF) share the listed paternity indices for each locus listed (LOC-A1, LOC-B2, LOC-C3, LOC-D4). Locus Tested PF Child Paternity Index LOC-A1 3 2/3 2.18 LOC-B2 7/5 5 0.798 LOC-C3 15/17 9/17 5.21 LOC-D4 12 12 1.37 Based on the data presented in the table, what is the combined paternity index, CPI, from the loci tested: LOC-A1, LOC-B2, LOC-C3 and LOC-D4? 12.42 9.558 15.56 2.38 12.42...

ASCP MB Exam Questions & Answers 2024 Which PCR method has the highest specificity? A. qPCR B. Touchdown PCR C. Nested PCR D. Two-step PCR - ANSWER-Nested PCR Given the anti-sense strand of DNA: 5'-AATTGCCGACATAGAT-3' which is the appropriate sense strand? A. 5'-ATCTATGTCGGCAATT-3' B. 5'-TTAACGGCTGTATCTA-3' C. 5'-AATTGCCGACATAGAT-3' D. 5'-GAGCACGCTATCTTAT-3' - ANSWER-5'-ATCTATGTCGGCAATT-3' During DNA replication, the 3' -OH of the growing DNA chain attacks wh...

What is the Beer-Lambert Law? - Answer the linear relationship between absorbance and concentration of an absorbing species. What is the Beer-Lambert Law equation? - Answer A = ebc A: absorbance e: molar absorptivity c: concentration b: path length DNA and RNA are absorbed at: - Answer 260nm Proteins are absorbed at: - Answer 280nm What is the melting temperature equation? - Answer Tm = (4 x # of GC pairs) + (2 x # of AT pairs) Dilutions equation - Answer V1C1 = V2C2 V1:...

MB (ASCP) Practice Exam 2024 100% Correct What does barcoding do in Next Generation Sequencing? - Answer ️️ -Adds unique tag to all nucleic acids within one sample so that various samples can be mixed What is the best way to store RNA for longer than a month? - Answer ️️ -In Ethanol, at -20°C or -80°C Testing for Aldehyde Dehydrogenase deficiency is important in patients with: - Answer ️️ - Alcohol dependence During DNA replication, the 3' -OH of the growing DNA chain attac...

Predominant form of DNA Right-handed B DNA and histone interaction is due to what type of bond? Ionic What stains to the minor groove of DNA duplex? SYBR Green What is the most abundant type of RNA? Ribosomal RNA (rRNA) What does RNA act as in a retrovirus? Genome and mRNA What increases half-life of mRNA? 5' Methyl Cap What kind of DNA is most likely to be transcriptionally active? Euchromatin What is the most common type of eukaryotic DNA? Satellite ...

MB (ASCP) Practice Exam 2024 100% Correct What does barcoding do in Next Generation Sequencing? - Answer ️️ -Adds unique tag to all nucleic acids within one sample so that various samples can be mixed What is the best way to store RNA for longer than a month? - Answer ️️ -In Ethanol, at -20°C or -80°C Testing for Aldehyde Dehydrogenase deficiency is important in patients with: - Answer ️️ - Alcohol dependence During DNA replication, the 3' -OH of the growing DNA chain attac...

MB (ASCP) Exam Questions and Answers 2023 Graded A+. Predominant form of DNA Right-handed B DNA and histone interaction is due to what type of bond? Ionic What stains to the minor groove of DNA duplex? SYBR Green What is the most abundant type of RNA? Ribosomal RNA (rRNA) What does RNA act as in a retrovirus? Genome and mRNA What increases half-life of mRNA? 5' Methyl Cap What kind of DNA is most likely to be transcriptionally active? Euchromatin What is the most common type of e...

Molecular Diagnostics Final (Exam + Quiz MC) /verified/ d (primers) *Ans* *The specificity of the PCR reaction is determined by which of the following components?* a. Mg2+ concentration b. Deoxynucleotides c. DNA polymerase d. Primers a (primer dimers) *Ans* *Complementarity of primer pairs at the 3' ends will result in what artifacts?* a. Primer dimers b. Large mis-primed products c. No amplification d. 5' end degradation of the primers d (secondary structure) *Ans* *A DNA se...

MB ASCP CONNECT Questions and Answers 100% correct What is translocation is associated with Burkitt's Lymphoma? a. t(18; 14) b. t(9; 22) c. t(8; 14) d. t(15; 17) t(8; 14) TIP: Burkkitt's 8 letters, Locus PF Child Paternity Index LOC-A1 3 2/3 2.18 LOC-B2 7/5 5 0.798 LOC-C3 17/17 9/17 5.21 LOC-D4 12 12 1.37 Based on the data presented in the table above and with a prior probability (PP) of 0.5, the probability of paternity in this case is : a. 92.5% b. 99.5% c. 90...

MB (ASCP) Exam (Answered) With Complete Verified Solution 100% Predominant form of DNA Right-handed B DNA and histone interaction is due to what type of bond? Ionic What stains to the minor groove of DNA duplex? SYBR Green What is the most abundant type of RNA? Ribosomal RNA (rRNA) What does RNA act as in a retrovirus? Genome and mRNA What increases half-life of mRNA? 5' Methyl Cap What kind of DNA is most likely to be transcriptionally active? Euchromatin What is the most comm...