Self study assignment: More recent and convenient cloning systems
Prof. Van Camp
Fundamentals of gene and genome technology
Gene and genome technology
Learning aids:
Several video’s and texts on Gateway cloning and Gibson assembly are available on Blackboard
Aims:
1) Gateway cloning
• The student can describe the CcdA/CcdB selection system in cloning with technical details
• The student can describe the Gateway cloning system with technical details and required
components
• The student can describe the advantages and disadvantages of the Gateway cloning system
• The student can list and explain the differences between Gateway cloning and traditional
cloning using restriction enzymes and ligase
• The student can design a cloning experiment using the Gateway system (the student knows
which att sites to use in which stage of the cloning experiment)
2) Gibson assembly
• The student can describe the Gibson assembly with technical details and required compenents
• The student can describe the advantages and disadvantage of Gibson assembly
• The student can incorporate Gibson assembly in the theoretical design of cloning experiments
to replace restriction enzyme/ligation steps
Prof. Van Camp
Fundamentals of gene and genome technology
Gene and genome technology
Learning aids:
Several video’s and texts on Gateway cloning and Gibson assembly are available on Blackboard
Aims:
1) Gateway cloning
• The student can describe the CcdA/CcdB selection system in cloning with technical details
• The student can describe the Gateway cloning system with technical details and required
components
• The student can describe the advantages and disadvantages of the Gateway cloning system
• The student can list and explain the differences between Gateway cloning and traditional
cloning using restriction enzymes and ligase
• The student can design a cloning experiment using the Gateway system (the student knows
which att sites to use in which stage of the cloning experiment)
2) Gibson assembly
• The student can describe the Gibson assembly with technical details and required compenents
• The student can describe the advantages and disadvantage of Gibson assembly
• The student can incorporate Gibson assembly in the theoretical design of cloning experiments
to replace restriction enzyme/ligation steps
