Gene and Genome Technology
13/01/20 - 08u30 group 1
5 questions. 1 oral, 4 written.
Oral: You discovered a new bird species and want to sequence the complete genome. Which
technique would you use? Which ones wouldn't be good and why?
Written 1: array-CGH vs. SNP-array. What are the similarities and differences of these
techniques used for finding deletions and duplications?
Written 2: PacBIO and Nanopore; both techniques that are able to sequence long sequences.
Choose one to explain and explain why they're able to read long sequences.
3: a) You want to clone a PCR product into a specific vector into an EcoRI site. The PCR
product however doesn't have an EcoRI site. How would you solve this knowing that it has to
go in the EcoRI site? (max 10 lines)
Written 3: b) List the different cloning vectors, the sizes of the insert for each and best known
applications for each.
Written 4: In evolution, how do gene duplications occur and what will happen to these genes after
a few generations?
14/01 - 8u30
1) Ion proton sequencing. Why difficulties with long stretches of homosequences (AAAAAAAA or
GGGGGGG)
2) A) …
B) site directed mutagenesis + drawing
3) Relevance of genotyping of CYP2D6 and CYP3A4 in context pharmacogenomics
4) Explain
a) Exon shuffling
b) Paralogue
c) TPMT
d) Mycoplasma genitalia
5) Oral. Human protein expression in T cells.. Not a lot known. Goal: purification. Strategy?
16/01 - 13u00
1) A clinician comes to you because she found a patient that has the clinical symptoms of a
specific genomic disease. This disease has a deletion of a CNV including 5 genes. You use a
SNP assay to look at the DNA of your patient and you discover no such deletion of a CNV in
this region. What would you tell the clinician to test next?
2) a) A clinician wants to do a mutation analysis in the coding region of a gene. This gene
consists of only one coding exon. They want to do a PCR, a cloning, pick up a clone and do
Sanger sequencing. What is the problem with this, what did they forget? How can you resolve
this problem?
13/01/20 - 08u30 group 1
5 questions. 1 oral, 4 written.
Oral: You discovered a new bird species and want to sequence the complete genome. Which
technique would you use? Which ones wouldn't be good and why?
Written 1: array-CGH vs. SNP-array. What are the similarities and differences of these
techniques used for finding deletions and duplications?
Written 2: PacBIO and Nanopore; both techniques that are able to sequence long sequences.
Choose one to explain and explain why they're able to read long sequences.
3: a) You want to clone a PCR product into a specific vector into an EcoRI site. The PCR
product however doesn't have an EcoRI site. How would you solve this knowing that it has to
go in the EcoRI site? (max 10 lines)
Written 3: b) List the different cloning vectors, the sizes of the insert for each and best known
applications for each.
Written 4: In evolution, how do gene duplications occur and what will happen to these genes after
a few generations?
14/01 - 8u30
1) Ion proton sequencing. Why difficulties with long stretches of homosequences (AAAAAAAA or
GGGGGGG)
2) A) …
B) site directed mutagenesis + drawing
3) Relevance of genotyping of CYP2D6 and CYP3A4 in context pharmacogenomics
4) Explain
a) Exon shuffling
b) Paralogue
c) TPMT
d) Mycoplasma genitalia
5) Oral. Human protein expression in T cells.. Not a lot known. Goal: purification. Strategy?
16/01 - 13u00
1) A clinician comes to you because she found a patient that has the clinical symptoms of a
specific genomic disease. This disease has a deletion of a CNV including 5 genes. You use a
SNP assay to look at the DNA of your patient and you discover no such deletion of a CNV in
this region. What would you tell the clinician to test next?
2) a) A clinician wants to do a mutation analysis in the coding region of a gene. This gene
consists of only one coding exon. They want to do a PCR, a cloning, pick up a clone and do
Sanger sequencing. What is the problem with this, what did they forget? How can you resolve
this problem?
