DNA Replication 7. The replication of chromosomes by eukaryotes occurs in
a relatively short period of time because
1. Both strands of DNA serve as templates concurrently in
A. the eukaryotes have more amount of DNA for
A. replication replication
B. excision repair B. the eukaryotic replication machinery is 1000 times faster
C. mismatch repair than the prokaryotes
D. none of these C. each chromosome contains multiple replicons
D. eukaryotic DNA is always single stranded
2. Proofreading activity to maintain the fidelity of DNA
synthesis 8. In E.coli, which enzyme synthesizes the RNA primer for
Okazaki fragments?
A. occurs after the synthesis has been completed
B. is a function of the 3'-5' exonuclease activity of the DNA A. DnaA
polymerases B. DnaC
C. requires the presence of an enzyme separate from the C. DnaG
DNA polymerases D. all of these
D. occurs in prokaryotes but not eukaryotes
9. During which of the following process a new copy of a
3. Which of the following repairs nicked DNA by forming a DNA molecule is precisely synthesized?
phosphodiester bond between adjacent nucleotides?
A. Trasformation
A. Helicase B. Transcription
B. DNA gyrase C. Translation
C. Topoisomerases D. Replication
D. DNA ligase
10. DNA gyrase is inhibited by
4. The enzyme that catalyzes the synthesis of DNA is called
A. tetracycline
A. DNA polymerase B. nalidixic acid
B. DNA gyrase C. both (a) and (b)
C. DNA ligase D. cephalosporin
D. helicase
11. Which of the following enzymes unwind short stretches
5. Which of the following possesses both 5'-3' and 3'-5' of DNA helix immediately ahead of a replication fork?
exonuclease activity?
A. DNA polymerases
A. Kornberg enzyme B. Helicases
B. DNA polymerase III C. Single-stranded binding proteins
C. Taq DNA polymerase D. Topoisomerases
D. None of these
12. During DNA replication in prokaryotes, synthesis begins
6. Which of the following statements on replication in on the circular chromosome
E.coli is correct?
A. always at the same place
A. It occurs in a unidirectional manner B. at any stretch of DNA that is high in AT pairs
B. It occurs in bidirectional manner C. randomly on the chromosome
C. Always uses T7 DNA polymerase when infected by T7 D. at the promoter
phage
D. Occurs only when λ phase has infected E. coli
a relatively short period of time because
1. Both strands of DNA serve as templates concurrently in
A. the eukaryotes have more amount of DNA for
A. replication replication
B. excision repair B. the eukaryotic replication machinery is 1000 times faster
C. mismatch repair than the prokaryotes
D. none of these C. each chromosome contains multiple replicons
D. eukaryotic DNA is always single stranded
2. Proofreading activity to maintain the fidelity of DNA
synthesis 8. In E.coli, which enzyme synthesizes the RNA primer for
Okazaki fragments?
A. occurs after the synthesis has been completed
B. is a function of the 3'-5' exonuclease activity of the DNA A. DnaA
polymerases B. DnaC
C. requires the presence of an enzyme separate from the C. DnaG
DNA polymerases D. all of these
D. occurs in prokaryotes but not eukaryotes
9. During which of the following process a new copy of a
3. Which of the following repairs nicked DNA by forming a DNA molecule is precisely synthesized?
phosphodiester bond between adjacent nucleotides?
A. Trasformation
A. Helicase B. Transcription
B. DNA gyrase C. Translation
C. Topoisomerases D. Replication
D. DNA ligase
10. DNA gyrase is inhibited by
4. The enzyme that catalyzes the synthesis of DNA is called
A. tetracycline
A. DNA polymerase B. nalidixic acid
B. DNA gyrase C. both (a) and (b)
C. DNA ligase D. cephalosporin
D. helicase
11. Which of the following enzymes unwind short stretches
5. Which of the following possesses both 5'-3' and 3'-5' of DNA helix immediately ahead of a replication fork?
exonuclease activity?
A. DNA polymerases
A. Kornberg enzyme B. Helicases
B. DNA polymerase III C. Single-stranded binding proteins
C. Taq DNA polymerase D. Topoisomerases
D. None of these
12. During DNA replication in prokaryotes, synthesis begins
6. Which of the following statements on replication in on the circular chromosome
E.coli is correct?
A. always at the same place
A. It occurs in a unidirectional manner B. at any stretch of DNA that is high in AT pairs
B. It occurs in bidirectional manner C. randomly on the chromosome
C. Always uses T7 DNA polymerase when infected by T7 D. at the promoter
phage
D. Occurs only when λ phase has infected E. coli
