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FIN 306 NPV mid term exam-1 | fall 2025/26 Update with complete solutions.

Henderson-Hasselbach Equation - pH = pKa + log ([A-] / [HA]) Salting Out (Purification) - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - Separates sample based on size with smaller molecules eluting later. Ion-Exchange Chromatography - Separates sample based on charge. CM attracts +, DEAE attracts -. May have repulsion effect on like charges. Salt or acid used to remove st...

This document summarizes key ACS Biochemistry topics, including analytical techniques, purification methods, chemical reagents, and foundational equations such as the Henderson–Hasselbalch equation. It provides clear explanations of chromatography types, electrophoresis methods, and chemical modifications relevant to protein analysis. The content reflects updated material for the 2024/2025 exam cycle and includes accurate question-and-answer style notes.

This document covers essential ACS Biochemistry exam material, including detailed explanations of metabolism, catabolism, anabolism, and key cellular properties. It contains actual questions and answers updated for the 2024/2025 exam cycle. The material provides clear definitions, conceptual clarifications, and topic-specific insights aligned with common ACS exam objectives.

Henderson-Hasselbach Equation - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - Separates sample based on size with smaller molecules eluting later. Ion-Exchange Chro...

This document provides a structured summary of essential biochemistry foundations. It explains metabolism in detail, highlighting catabolism as energy-releasing pathways and anabolism as energy-consuming biosynthetic processes. The key properties of cells, including reproduction, mutation, environmental responsiveness, and efficiency, are outlined to show how cells function and adapt. Finally, the unique chemical properties of water—such as hydrogen bonding, high specific heat, heat of vaporiz...

This document provides a concise overview of essential biochemistry techniques used in protein purification and analysis. It covers theoretical principles and applications of the Henderson-Hasselbalch equation, FMOC synthesis, salting out, chromatography methods, and electrophoresis techniques. These notes summarize the main mechanisms and experimental uses, making it a practical study guide for exams and laboratory preparation.

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