ASCP BOC Ques ons/ Complete BOC: ASCP prep With
complete solu on Newest RATED A+ 100% CORRECT
Which enzyme catalyzes the conversion of starch to glucose and maltose? - Ans Amylase
Nephelometers measure light: - Ans Sca ered at a right angle to the light path
A serum sample demonstrates an elevated result when tested with the Jaffe reac!on. This
indicates: - Ans Renal func!onal impairment
A vaginal/rectal swab is collected from a pregnant pa!ent to screen for group B Streptococcus
coloniza!on. What is the best medium to use for specimen inocula!on? - Ans Lim Broth
When tes!ng a pa!ent for HIV an!body, what is used to confirm a posi!ve screening test? -
Ans Western Blot
An isolate from a stool culture gives the following growth characteris!cs and biochemical
reac!ons:
MAC: Colorless colonies
HE agar: Yellow-Orange colonies
TSI: Acid slant/acid bu , no gas, no h2s
Urea: Posi!ve
These screening results are consistent with which enteric pathogen? - Ans Yersinia
entercoli!ca. Yersinia produces yellow colonies on HE because it can ferment sucrose.
, When stool examina!on is nega!ve, the preferred specimen for the diagnosis of paragonimiasis
is: - Ans Sputum. Paragonimus worms live in cys!c cavi!es in the lungs. Ova may be found in
sputum, or swallowed and passed in stool.
A branching gram-posi!ve, par!ally acid-fast organism is isolated from a bronchial washing on a
63-year-old woman receiving chemotherapy. The organism does not hydrolyze casein, tyrosine
or xanthine. The most likely iden!fica!on is: - Ans Nocardia asteroides. (THINK BECAUSE OF
THE BRANCHING). It is par!ally acid fast and does not hydrolyze the substrates casein, tyrosine,
or xanthine.
Use of only male donors as a source of plasma intended for transfusion is advocated to reduce
which type of reac!on? - Ans TRALI. TRALI is most commonly caused by donor HLA or
granulocyte-specific an!bodies that react with recipient an!gens, causing damage to the lung
basement membrane and bilateral pulmonary edema within 6 hours of transfusion. Mul!parous
females are more likely to have an!bodies than males. Using male donors as the sole source of
plasma products is a strategy for reducing the risk of TRALI.
The result of an an!-nuclear an!body test was a !ter of 1:320 with a peripheral pa ern. What
best correlates with these results? - Ans An!-dsDNA Titer 1:80. and a high !ter of an!bodies to
Sm. Peripheral pa ern reacts with the an!genic determinants of double strandedness of DNA.
Abnormali!es found in erythroleukemia include: - Ans Megaloblastoid development.
Which factor will not interfere with the reagent strip test for leukocytes in urine? - Ans Nitrite.
Nitrite is produced by bacteria, and WBCs are found when there is a bacterial infec!on. If nitrite
interfered with the WBC reac!on, then the dips!ck would be worthless when tes!ng pa!ents
with UTIs.
Which locus is used to iden!fy the gender of the individual from whom a DNA sample is
obtained? - Ans Amelogenin. Amelogenin gives different sized amplicons based on whether it
is amplified from the X or Y chromosomes, so males would have 2 bands on a gel at the
amelogenin locus, while females would have only 1.
, Penicillium species is isolated from a bone marrow culture of a pa!ent that travelled to
southeast Asia. ADer 7 days the isolate produces a red pigment that diffuses into the medium.
The technologist should: - Ans Transfer a colony to BHI at 35 degrees. C. Penicillium marneffiei
is a dimorphic fungi that produces a diffusible red pigment.
An elevated serum iron with normal iron binding capacity is most likely associated with: - Ans
Pernicious anemia. Ineffec!ve erythropoiesis. High iron turnover.
Which enzyme substrate is the most specific for prosta!c acid phosphatase for quan!ta!ve
endpoint reac!ons? - Ans Thymolphthalein monophosphate. Thymolphthalein
monophosphate is the most specific substrate of choice for quan!ta!ve endpoint reac!ons;
however, p-nitrophenylphosphate is the preferred substrate for con!nuous monitoring.
The principle of the tablet test for bilirubin in urine or feces is: - Ans Chemical coupling of
bilirubin with a diazonium salt to form a purple color.
Ghost red blood cells are seen in urine that is: - Ans Alkaline and dilute. RBCs absorb water
when in dilute urine, and are also less preserved in alkaline urine. Therefore, as the cells swell in
the alkaline urine, the cell membrane allows hemoglobin to leak from the cell, resul!ng in the
empty cell membrane and the pale appearance.
In the cold agglu!nin test, the tubes containing the serum and erythrocytes are allowed to
stand overnight in the refrigerator, and the results are read the next morning. If a disk of the
erythrocytes floats up from the bo om of the tube with only the flick of finger, this is read as a:
- Ans A 4+ reac!on. When one solid agglu!nate is present, the reac!on is classified as 4+.
A bone marrow shows foam cells ranging from 20-100 μm in size, vacuolated cytoplasm
containing sphingomyelin and is faintly PAS+. This cell type is most characteris!c of: - Ans
Niemann-Pick Disease
, The presence of leukocytes in urine is known as: - Ans Pyuria
In what metabolic disease will urine turn dark brown to black upon standing? - Ans
Alkaptonuria
First-genera!on cephalosporins can be adequately represented by: - Ans Cephalothin
According to AABB Standards, Platelets prepared from Whole Blood shall have at least: - Ans
5.5x10^10 platelets per unit in at least 90% of the units tested
A stool specimen that appears black and tarry should be tested for the presence of: - Ans
Occult blood
Sodium bicarbonate and sodium citrate are components of which of the following? - Ans
JEMBEC system. The JEMBEC system is a transport and inocula!on medium used for direct
pla!ng of specimens for N gonorrhoeae. Chocolate based selec!ve medium is inoculated with
the specimen. This is placed in an impermeable plas!c bag with a bicarbonatesodium citrate
pellet that absorbs moisture to generate a carbon dioxide-rich environment.
A 44-year-old man was admi ed to the hospital following a 2-week history of low-grade fever,
malaise and anorexia. Examina!on of a Giemsa stain revealed many intraerythrocy!c parasites.
Further history revealed frequent camping trips near Martha's Vineyard and Nantucket Island,
but no travel outside the con!nental United States. This parasite could easily be confused with:
- Ans Plasmodium falciparum. The pa!ent's history is sugges!ve of Babesia infec!on. Babesia
micro! ring forms are similar to Plasmodium falciparum. A travel history is helpful in
determining the cause of infec!on.
Platelets prepared in a polyolefin type container, stored at 22° - 24°C in 50 mL of plasma, and
gently agitated can be used for up to: - Ans 5 days.
complete solu on Newest RATED A+ 100% CORRECT
Which enzyme catalyzes the conversion of starch to glucose and maltose? - Ans Amylase
Nephelometers measure light: - Ans Sca ered at a right angle to the light path
A serum sample demonstrates an elevated result when tested with the Jaffe reac!on. This
indicates: - Ans Renal func!onal impairment
A vaginal/rectal swab is collected from a pregnant pa!ent to screen for group B Streptococcus
coloniza!on. What is the best medium to use for specimen inocula!on? - Ans Lim Broth
When tes!ng a pa!ent for HIV an!body, what is used to confirm a posi!ve screening test? -
Ans Western Blot
An isolate from a stool culture gives the following growth characteris!cs and biochemical
reac!ons:
MAC: Colorless colonies
HE agar: Yellow-Orange colonies
TSI: Acid slant/acid bu , no gas, no h2s
Urea: Posi!ve
These screening results are consistent with which enteric pathogen? - Ans Yersinia
entercoli!ca. Yersinia produces yellow colonies on HE because it can ferment sucrose.
, When stool examina!on is nega!ve, the preferred specimen for the diagnosis of paragonimiasis
is: - Ans Sputum. Paragonimus worms live in cys!c cavi!es in the lungs. Ova may be found in
sputum, or swallowed and passed in stool.
A branching gram-posi!ve, par!ally acid-fast organism is isolated from a bronchial washing on a
63-year-old woman receiving chemotherapy. The organism does not hydrolyze casein, tyrosine
or xanthine. The most likely iden!fica!on is: - Ans Nocardia asteroides. (THINK BECAUSE OF
THE BRANCHING). It is par!ally acid fast and does not hydrolyze the substrates casein, tyrosine,
or xanthine.
Use of only male donors as a source of plasma intended for transfusion is advocated to reduce
which type of reac!on? - Ans TRALI. TRALI is most commonly caused by donor HLA or
granulocyte-specific an!bodies that react with recipient an!gens, causing damage to the lung
basement membrane and bilateral pulmonary edema within 6 hours of transfusion. Mul!parous
females are more likely to have an!bodies than males. Using male donors as the sole source of
plasma products is a strategy for reducing the risk of TRALI.
The result of an an!-nuclear an!body test was a !ter of 1:320 with a peripheral pa ern. What
best correlates with these results? - Ans An!-dsDNA Titer 1:80. and a high !ter of an!bodies to
Sm. Peripheral pa ern reacts with the an!genic determinants of double strandedness of DNA.
Abnormali!es found in erythroleukemia include: - Ans Megaloblastoid development.
Which factor will not interfere with the reagent strip test for leukocytes in urine? - Ans Nitrite.
Nitrite is produced by bacteria, and WBCs are found when there is a bacterial infec!on. If nitrite
interfered with the WBC reac!on, then the dips!ck would be worthless when tes!ng pa!ents
with UTIs.
Which locus is used to iden!fy the gender of the individual from whom a DNA sample is
obtained? - Ans Amelogenin. Amelogenin gives different sized amplicons based on whether it
is amplified from the X or Y chromosomes, so males would have 2 bands on a gel at the
amelogenin locus, while females would have only 1.
, Penicillium species is isolated from a bone marrow culture of a pa!ent that travelled to
southeast Asia. ADer 7 days the isolate produces a red pigment that diffuses into the medium.
The technologist should: - Ans Transfer a colony to BHI at 35 degrees. C. Penicillium marneffiei
is a dimorphic fungi that produces a diffusible red pigment.
An elevated serum iron with normal iron binding capacity is most likely associated with: - Ans
Pernicious anemia. Ineffec!ve erythropoiesis. High iron turnover.
Which enzyme substrate is the most specific for prosta!c acid phosphatase for quan!ta!ve
endpoint reac!ons? - Ans Thymolphthalein monophosphate. Thymolphthalein
monophosphate is the most specific substrate of choice for quan!ta!ve endpoint reac!ons;
however, p-nitrophenylphosphate is the preferred substrate for con!nuous monitoring.
The principle of the tablet test for bilirubin in urine or feces is: - Ans Chemical coupling of
bilirubin with a diazonium salt to form a purple color.
Ghost red blood cells are seen in urine that is: - Ans Alkaline and dilute. RBCs absorb water
when in dilute urine, and are also less preserved in alkaline urine. Therefore, as the cells swell in
the alkaline urine, the cell membrane allows hemoglobin to leak from the cell, resul!ng in the
empty cell membrane and the pale appearance.
In the cold agglu!nin test, the tubes containing the serum and erythrocytes are allowed to
stand overnight in the refrigerator, and the results are read the next morning. If a disk of the
erythrocytes floats up from the bo om of the tube with only the flick of finger, this is read as a:
- Ans A 4+ reac!on. When one solid agglu!nate is present, the reac!on is classified as 4+.
A bone marrow shows foam cells ranging from 20-100 μm in size, vacuolated cytoplasm
containing sphingomyelin and is faintly PAS+. This cell type is most characteris!c of: - Ans
Niemann-Pick Disease
, The presence of leukocytes in urine is known as: - Ans Pyuria
In what metabolic disease will urine turn dark brown to black upon standing? - Ans
Alkaptonuria
First-genera!on cephalosporins can be adequately represented by: - Ans Cephalothin
According to AABB Standards, Platelets prepared from Whole Blood shall have at least: - Ans
5.5x10^10 platelets per unit in at least 90% of the units tested
A stool specimen that appears black and tarry should be tested for the presence of: - Ans
Occult blood
Sodium bicarbonate and sodium citrate are components of which of the following? - Ans
JEMBEC system. The JEMBEC system is a transport and inocula!on medium used for direct
pla!ng of specimens for N gonorrhoeae. Chocolate based selec!ve medium is inoculated with
the specimen. This is placed in an impermeable plas!c bag with a bicarbonatesodium citrate
pellet that absorbs moisture to generate a carbon dioxide-rich environment.
A 44-year-old man was admi ed to the hospital following a 2-week history of low-grade fever,
malaise and anorexia. Examina!on of a Giemsa stain revealed many intraerythrocy!c parasites.
Further history revealed frequent camping trips near Martha's Vineyard and Nantucket Island,
but no travel outside the con!nental United States. This parasite could easily be confused with:
- Ans Plasmodium falciparum. The pa!ent's history is sugges!ve of Babesia infec!on. Babesia
micro! ring forms are similar to Plasmodium falciparum. A travel history is helpful in
determining the cause of infec!on.
Platelets prepared in a polyolefin type container, stored at 22° - 24°C in 50 mL of plasma, and
gently agitated can be used for up to: - Ans 5 days.
