BIOL Chapter 9 - Practice
Study online at https://quizlet.com/_5nkzrv
1. At which site does the charged initiator tRNA P site
bind during protein synthesis?
2. Peptidyl transferase enzyme that catalyzes the formation
of peptide bonds during translation
3. EF‑Tu elongation factor that facilitates the
entry of charged tRNAs into the A site
4. What event occurs during translocation? mRNA shifts in the 5' direction along
the ribosome
5. Although the process of translation is similar in In bacteria, the start codon lies just
bacteria and eukaryotes, there are some impor- downstream of a recognition se-
tant differences quence called the Shine-Dalgarno
sequence. In eukaryotes, the start
codon is located within a different
recognition sequence, the Kozak se-
quence.
In bacteria, the amino acid on the
initiator tRNA is a modified me-
thionine called N-formylmethionine
(fMet). In eukaryotes, the amino acid
on the initiator tRNA is methionine.
6. In bacteria, a 70S initiation complex is assem-
bled from the two ribosomal subunits, mRNA,
and initiator tRNA with the assistance of three
initiation factors and energy from GTP.
7.
1/6
, BIOL Chapter 9 - Practice
Study online at https://quizlet.com/_5nkzrv
Wild-type strains of E. coli have a tRNA with
a 5′-GUA-3′ anticodon that recognizes the UAC
codon for tyrosine. We can represent this tRNA
as GUA-tRNATyr.
Suppose you isolated a strain of E. coli in which
a mutation changed the anticodon in this tRNA
to 5′-CUA-3′ (creating CUA-tRNATyr). Answer the
following questions about translation elonga-
tion in each strain.
8. In bacteria, the methionine that initiates the for- a formyl group is attached to the ini-
mation of a polypeptide chain differs from sub- tiating methionine
sequently added methionines in that _______.
9. Translation in bacteria is directly dependent on complementary base pairing be-
all of the following associations except _______. tween mRNA and DNA (transcription
is dependent on this)
10. Which of the following best describes the first The small ribosomal subunit binds to
step in the formation of the translation initiation an mRNA sequence near the 5' end
complex? of the transcript
11. Once elongation is underway, tRNAs involved A Site, P Site, E Site
in the process occupy a series of sites on the
complexed ribosome. The occupation of sites
occurs in the following order.
12. When a peptide bond is formed between two is attached to the tRNA occupying the
amino acids, one is attached to the tRNA occu- A site
pying the P site and the other _______.
13.
2/6
Study online at https://quizlet.com/_5nkzrv
1. At which site does the charged initiator tRNA P site
bind during protein synthesis?
2. Peptidyl transferase enzyme that catalyzes the formation
of peptide bonds during translation
3. EF‑Tu elongation factor that facilitates the
entry of charged tRNAs into the A site
4. What event occurs during translocation? mRNA shifts in the 5' direction along
the ribosome
5. Although the process of translation is similar in In bacteria, the start codon lies just
bacteria and eukaryotes, there are some impor- downstream of a recognition se-
tant differences quence called the Shine-Dalgarno
sequence. In eukaryotes, the start
codon is located within a different
recognition sequence, the Kozak se-
quence.
In bacteria, the amino acid on the
initiator tRNA is a modified me-
thionine called N-formylmethionine
(fMet). In eukaryotes, the amino acid
on the initiator tRNA is methionine.
6. In bacteria, a 70S initiation complex is assem-
bled from the two ribosomal subunits, mRNA,
and initiator tRNA with the assistance of three
initiation factors and energy from GTP.
7.
1/6
, BIOL Chapter 9 - Practice
Study online at https://quizlet.com/_5nkzrv
Wild-type strains of E. coli have a tRNA with
a 5′-GUA-3′ anticodon that recognizes the UAC
codon for tyrosine. We can represent this tRNA
as GUA-tRNATyr.
Suppose you isolated a strain of E. coli in which
a mutation changed the anticodon in this tRNA
to 5′-CUA-3′ (creating CUA-tRNATyr). Answer the
following questions about translation elonga-
tion in each strain.
8. In bacteria, the methionine that initiates the for- a formyl group is attached to the ini-
mation of a polypeptide chain differs from sub- tiating methionine
sequently added methionines in that _______.
9. Translation in bacteria is directly dependent on complementary base pairing be-
all of the following associations except _______. tween mRNA and DNA (transcription
is dependent on this)
10. Which of the following best describes the first The small ribosomal subunit binds to
step in the formation of the translation initiation an mRNA sequence near the 5' end
complex? of the transcript
11. Once elongation is underway, tRNAs involved A Site, P Site, E Site
in the process occupy a series of sites on the
complexed ribosome. The occupation of sites
occurs in the following order.
12. When a peptide bond is formed between two is attached to the tRNA occupying the
amino acids, one is attached to the tRNA occu- A site
pying the P site and the other _______.
13.
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