Molecular Diagnostics Final Exam
Rated A+
a (5') - ANSWER-*Which end of the sequenced DNA is found at the bottom of a
sequencing gel?*
a. 5'
b. 3'
c. 2'
d. 1'
a (8 mg/ml) - ANSWER-*A RNA prep diluted 1:1000 yields an absorbance reading of
0.2 at 260nm. What's the concentration of this RNA prep?*
a. 8 mg/ml
b. 10000 ug/ml
c. 8000 mg/ml
d. 10 mg/ml
a (ability to analyze specific regions of DNA without cloning them) - ANSWER-*What
was the great advantage to molecular analysis offered by Southern blot?*
a. Ability to analyze specific regions of DNA without cloning them
b. A rapid method to eliminate DNA contaminants
c. A more efficient buffer system
d. Ability to analyze nanogram amounts of DNA
a (an STR allele missing base pairs in the repeat unit) - ANSWER-*What is a
microvariant?*
a. An STR allele missing base pairs in the repeat unit
b. Any mini-STR allele
c. A viral genome integrated into host DNA
d. A DNA region with many HaeIII sites
a (BRCA1) - ANSWER-*A patient with a family history of breast cancer wants to know
her risk of having breast cancer. Which of the following genes should be analyzed in
this patient?*
a. BRCA1
b. Von Hippel-Lindau
c. N-myc
d. K-ras
a (capillary electrophoresis) - ANSWER-*A very limited amount of nucleic acid, 500-
1500 bp in size, is to be analyzed in a short time (same day) with the results available
immediately. Which of the following electrophoresis procedures will satisfy those
conditions?*
a. Capillary electrophoresis
,b. Pulse-field gel electrophoresis
c. Field inversion gel electrophoresis
d. Contour-clamped homogeneous electric field
a (CGH arrays detect gain or loss of DNA regions; gene expression arrays detect
changes in gene expression) - ANSWER-*How do gene expression arrays differ from
comparative genome hybridization (CGH) arrays?*
a. CGH arrays detect gain or loss of DNA regions; gene expression arrays detect
changes in gene expression
b. CGH arrays can interrogate hundreds of genes; while genes expression arrays test
thousands
c. CGH arrays are simpler to perform than gene expression arrays
d. CGH arrays require co-hybridization with comparative reference nucleic acid, while
gene expression arrays do not
a (dot blot) - ANSWER-*Which of the following methods is best suited for qualitative
analysis of DNA, such as mutational analysis, where many samples are compared
simultaneously?*
a. Dot Blot
b. Traditional southern blot
c. Western blot
d. Northern blot
a (family history) - ANSWER-*How are transmission patterns determined?*
a. Family history
b. Karyotyping
c. Environmental studies
d. Comparing unrelated individuals
a (female) - ANSWER-*DNA found at the scene of the crime was determined to have
one peak at 212 bp at the amelogenin locus. The source of this DNA is which of the
following?*
a. Female
b. Male
c. Impossible to tell from one locus
d. This locus does not discriminate between males and females.
a (find blocks of SNPs that are inherited together) - ANSWER-*The goal of the HapMap
project is which of the following?*
a. Find blocks of SNPs that are inherited together
b. Complete the human genome sequence
c. Compare human and primate genomes
d. Sequence all bacterial genomes
a (G bands) - ANSWER-*When chromosomes are stained with Giemsa, the resulting
bands are called:*
, a. G bands
b. Q bands
c. R bands
d. C bands
a (H1) - ANSWER-*There are five major types of histones in eukaryotic cells. One of
these is not part of the structure of nucleosomes and is thought to participate in forming
the 30-nm condensed chromatin fiber. Which histone is this?*
a. H1
b. H2A and B
c. H3
d. H4
a (luminometer) - ANSWER-*A pyrosequencer detects visible light, making it which type
of instrument?*
a. Luminometer
b. UV spectrotometer
c. Mass spectrometer
d. Fluorometer
a (lyse the cell membrane) - ANSWER-*In DNA extraction, the purpose of adding SDS
step is to:*
a. Lyse the cell membrane
b. Precipitate the proteins
c. Precipitate the DNA to concentrate it
d. Precipitate the DNA to remove contaminants
a (microdeletion) - ANSWER-*Which of the following would be most difficult to identify
by karyotype?*
a. Microdeletion
b. Reciprocal translocation
c. Aneuploidy
d. Polyploidy
a (mitochondrial related) - ANSWER-*A pedigree was examined over four generations
of a family with an abnormal rate of genetic disease. Scientists observed that males and
females alike were affected by the disease but only when their mother was also
affected. The children of affected males were all normal. All the children of affected
females were affected. Which of the following describes this inheritance pattern?*
a. Mitochondrial related
b. Autosomal dominant
c. Autosomal recessive
d. Sex linked
a (monitor development of antiretroviral drug resistance) - ANSWER-*HIV genotyping is
performed for which of the following reasons?*
Rated A+
a (5') - ANSWER-*Which end of the sequenced DNA is found at the bottom of a
sequencing gel?*
a. 5'
b. 3'
c. 2'
d. 1'
a (8 mg/ml) - ANSWER-*A RNA prep diluted 1:1000 yields an absorbance reading of
0.2 at 260nm. What's the concentration of this RNA prep?*
a. 8 mg/ml
b. 10000 ug/ml
c. 8000 mg/ml
d. 10 mg/ml
a (ability to analyze specific regions of DNA without cloning them) - ANSWER-*What
was the great advantage to molecular analysis offered by Southern blot?*
a. Ability to analyze specific regions of DNA without cloning them
b. A rapid method to eliminate DNA contaminants
c. A more efficient buffer system
d. Ability to analyze nanogram amounts of DNA
a (an STR allele missing base pairs in the repeat unit) - ANSWER-*What is a
microvariant?*
a. An STR allele missing base pairs in the repeat unit
b. Any mini-STR allele
c. A viral genome integrated into host DNA
d. A DNA region with many HaeIII sites
a (BRCA1) - ANSWER-*A patient with a family history of breast cancer wants to know
her risk of having breast cancer. Which of the following genes should be analyzed in
this patient?*
a. BRCA1
b. Von Hippel-Lindau
c. N-myc
d. K-ras
a (capillary electrophoresis) - ANSWER-*A very limited amount of nucleic acid, 500-
1500 bp in size, is to be analyzed in a short time (same day) with the results available
immediately. Which of the following electrophoresis procedures will satisfy those
conditions?*
a. Capillary electrophoresis
,b. Pulse-field gel electrophoresis
c. Field inversion gel electrophoresis
d. Contour-clamped homogeneous electric field
a (CGH arrays detect gain or loss of DNA regions; gene expression arrays detect
changes in gene expression) - ANSWER-*How do gene expression arrays differ from
comparative genome hybridization (CGH) arrays?*
a. CGH arrays detect gain or loss of DNA regions; gene expression arrays detect
changes in gene expression
b. CGH arrays can interrogate hundreds of genes; while genes expression arrays test
thousands
c. CGH arrays are simpler to perform than gene expression arrays
d. CGH arrays require co-hybridization with comparative reference nucleic acid, while
gene expression arrays do not
a (dot blot) - ANSWER-*Which of the following methods is best suited for qualitative
analysis of DNA, such as mutational analysis, where many samples are compared
simultaneously?*
a. Dot Blot
b. Traditional southern blot
c. Western blot
d. Northern blot
a (family history) - ANSWER-*How are transmission patterns determined?*
a. Family history
b. Karyotyping
c. Environmental studies
d. Comparing unrelated individuals
a (female) - ANSWER-*DNA found at the scene of the crime was determined to have
one peak at 212 bp at the amelogenin locus. The source of this DNA is which of the
following?*
a. Female
b. Male
c. Impossible to tell from one locus
d. This locus does not discriminate between males and females.
a (find blocks of SNPs that are inherited together) - ANSWER-*The goal of the HapMap
project is which of the following?*
a. Find blocks of SNPs that are inherited together
b. Complete the human genome sequence
c. Compare human and primate genomes
d. Sequence all bacterial genomes
a (G bands) - ANSWER-*When chromosomes are stained with Giemsa, the resulting
bands are called:*
, a. G bands
b. Q bands
c. R bands
d. C bands
a (H1) - ANSWER-*There are five major types of histones in eukaryotic cells. One of
these is not part of the structure of nucleosomes and is thought to participate in forming
the 30-nm condensed chromatin fiber. Which histone is this?*
a. H1
b. H2A and B
c. H3
d. H4
a (luminometer) - ANSWER-*A pyrosequencer detects visible light, making it which type
of instrument?*
a. Luminometer
b. UV spectrotometer
c. Mass spectrometer
d. Fluorometer
a (lyse the cell membrane) - ANSWER-*In DNA extraction, the purpose of adding SDS
step is to:*
a. Lyse the cell membrane
b. Precipitate the proteins
c. Precipitate the DNA to concentrate it
d. Precipitate the DNA to remove contaminants
a (microdeletion) - ANSWER-*Which of the following would be most difficult to identify
by karyotype?*
a. Microdeletion
b. Reciprocal translocation
c. Aneuploidy
d. Polyploidy
a (mitochondrial related) - ANSWER-*A pedigree was examined over four generations
of a family with an abnormal rate of genetic disease. Scientists observed that males and
females alike were affected by the disease but only when their mother was also
affected. The children of affected males were all normal. All the children of affected
females were affected. Which of the following describes this inheritance pattern?*
a. Mitochondrial related
b. Autosomal dominant
c. Autosomal recessive
d. Sex linked
a (monitor development of antiretroviral drug resistance) - ANSWER-*HIV genotyping is
performed for which of the following reasons?*
