Blood Bank Rotation Practice
questions, Dr. Walz Questions with
100% Correct Answers
A2 negative Correct Answer: Forard typing: Anti-A 3+. Anti-B negative. Anti- A,B
3+. Anti- D negative. Rh control negative
Reverse typing: A1 cell negative. B cell negative.
A1B negative, genotyp: dce/dce Correct Answer: Forward typing: Anti-A
negative. Anti-B 4+. Anti-A,B 4+. Anti-D negative. Weak D negative.
Genotyping: Anti-C negative, Anti-E negative, Anti-c 3+, Anti-e 3+
A1B positive Correct Answer: Forward typing: Anti-A negative. Anti-B 4+. Anti-A,B
4+. Anti-D 3+.
Reverse typing: A1cells 3+. Bcells negative.
O positive
genotype: DCe/dce Correct Answer: Forward typing: Anti-A negative. Anti-B
negative. Anti-A,B negative. Anti-D negative. Rh control negative.
Genotyping: Weak D 2+. Weak D control negative. Anti-C 2+. Anti-E negative.
Anti-c 3+. Anti-e 3+.
There is a suspicious weak anti-B. To ensure that this an 'imposter B', acidify a
small amount of antisera, then dforward type with it. If there is no agglutination,
then it is indeed an imposter B. Correct Answer: 63 year-old male w/colon
cancer, HgB value of 6.5 g/dl. Needs packed RBCs.
, Anti-A 4+, Anti-B 1+, Anti-A,B 4+, A1 cells negative, B cells 4+
4 units of C-negative blood
30% of the population are C negative
N= 4/.30 N=13.33 = 14
Crossmatch 14 units of blood to find 4 units that are antigen negative for C.
Correct Answer: An accident victim comes to the ER and needs blood for
surgery ASAP. In working up the patients' sample, and anti-C is identified in the
plasma. How would you go about finding 4 units of C negative blood to issue?
Which blood group antigens are most susceptible to denaturation from the
action of proteolytic enzymes, such as papain and ficin? Correct Answer: Duffy-
Fya and Fyb
If anti-c has been tentatively identified in the patient's serum, but anti-E, anti-K,
and anti-S have not been excluded, RBCs of what phenotypes would need to
be included as selected cells? Correct Answer: The selected cell would need to
be negative for little c and positive for E, K, and S.
Incomplete washing of the indirect Coombs test will result in false
(negative/positive) test results and subsequently (negative/positive) reactions
with check cells. Correct Answer: Negative, negative
List 4 limitations of the Immediate spin crossmatch: Correct Answer: 1) I.S.
crossmatch may detect clinically insignificant cold-reacting antibodies.
2) I.S. crossmatch may not be able to detect all incompatibilities with antibodies
that need work-ups.
3) I.S. crossmatch may not detect all ABO incompatibilities.
questions, Dr. Walz Questions with
100% Correct Answers
A2 negative Correct Answer: Forard typing: Anti-A 3+. Anti-B negative. Anti- A,B
3+. Anti- D negative. Rh control negative
Reverse typing: A1 cell negative. B cell negative.
A1B negative, genotyp: dce/dce Correct Answer: Forward typing: Anti-A
negative. Anti-B 4+. Anti-A,B 4+. Anti-D negative. Weak D negative.
Genotyping: Anti-C negative, Anti-E negative, Anti-c 3+, Anti-e 3+
A1B positive Correct Answer: Forward typing: Anti-A negative. Anti-B 4+. Anti-A,B
4+. Anti-D 3+.
Reverse typing: A1cells 3+. Bcells negative.
O positive
genotype: DCe/dce Correct Answer: Forward typing: Anti-A negative. Anti-B
negative. Anti-A,B negative. Anti-D negative. Rh control negative.
Genotyping: Weak D 2+. Weak D control negative. Anti-C 2+. Anti-E negative.
Anti-c 3+. Anti-e 3+.
There is a suspicious weak anti-B. To ensure that this an 'imposter B', acidify a
small amount of antisera, then dforward type with it. If there is no agglutination,
then it is indeed an imposter B. Correct Answer: 63 year-old male w/colon
cancer, HgB value of 6.5 g/dl. Needs packed RBCs.
, Anti-A 4+, Anti-B 1+, Anti-A,B 4+, A1 cells negative, B cells 4+
4 units of C-negative blood
30% of the population are C negative
N= 4/.30 N=13.33 = 14
Crossmatch 14 units of blood to find 4 units that are antigen negative for C.
Correct Answer: An accident victim comes to the ER and needs blood for
surgery ASAP. In working up the patients' sample, and anti-C is identified in the
plasma. How would you go about finding 4 units of C negative blood to issue?
Which blood group antigens are most susceptible to denaturation from the
action of proteolytic enzymes, such as papain and ficin? Correct Answer: Duffy-
Fya and Fyb
If anti-c has been tentatively identified in the patient's serum, but anti-E, anti-K,
and anti-S have not been excluded, RBCs of what phenotypes would need to
be included as selected cells? Correct Answer: The selected cell would need to
be negative for little c and positive for E, K, and S.
Incomplete washing of the indirect Coombs test will result in false
(negative/positive) test results and subsequently (negative/positive) reactions
with check cells. Correct Answer: Negative, negative
List 4 limitations of the Immediate spin crossmatch: Correct Answer: 1) I.S.
crossmatch may detect clinically insignificant cold-reacting antibodies.
2) I.S. crossmatch may not be able to detect all incompatibilities with antibodies
that need work-ups.
3) I.S. crossmatch may not detect all ABO incompatibilities.
