UMKC Biology 108 Lab Practical #1 QUESTIONS WITH
COMPLETE SOLUTIONS
A longer wavelength is associated with a lower or higher
resolution? Correct Answer Lower resolution
A scientifically useful hypothesis must be...? Correct Answer
testable and falsifiable (able to be proved false)
After separating the pigments, what was your objective?
Correct Answer Measure the absorbency of light
Amylase breaks starch down to what sugar? Correct Answer
Maltose
Anaphase Correct Answer Sister chromatids are separated and
move to opposite ends of the cell
Benedict's Reagent color changes from blue to yellow in the
presence of glucose. What chemical rxn causes this color
change? Correct Answer It's caused by the reduction of
copper
Cytokinesis Correct Answer Division of the cytoplasm
forming two daughter cells
Even though the hypothesis can be falsified, it can never be
proven true. The evidence from an investigation can only...?
Correct Answer provide support for the hypothesis
,For a question to be pursued by scientists, the phenomenon must
be _____? The elements must be _____? Correct Answer 1.
well defined and testable
2. measurable and controllable
How can you test a hypothesis? Correct Answer Experiments,
additional observations, synthesis of information available
How could you tell if amylase had a low activity level? Correct
Answer There was no change in color over a long period of
time.
How could you test to see if amylase was permanently denatured
at a high temperature? Correct Answer Lower the temperature
to an optimum level and see if the time of change or a change in
color occurs. If there is no change, amylase has been irreversibly
inactivated (denatured).
How did you know that starch did not diffuse out of the bag?
Correct Answer If starch had diffused out of the bag, it would
have reacted with the potassium iodide solution in the beaker
and turned it purple. We knew it did't because there was no color
change. We can assume starch was unable to permeate through
the bag.
How do we know if the sample of Benedict's Reagent actually
worked? Correct Answer We had a positive control in the
BAG SAMPLE. The bag sample contained a heavy
concentration of glucose and was expected to have changed
orange/red in color. The beaker solution also contained glucose,
be it in less concentration.
, How do we know when the enzyme was active? Correct
Answer "If there was slow change, the was low activity. If
there was fast change, there was high activity."
How do you calculate the retardation factor? Correct Answer
Migration distance of the substance / migration distance of the
solvent front
How do you calculate total magnification? Correct Answer
Eyepiece /optical lens x objective lens
How does adding oil between the specimen and objective lens
increase resolution? What do you change in the equation?
Correct Answer The oil increases dispersion of light, what is
changed is the refractive index.
If _____, then _____. Correct Answer 1. hypothesis
2. prediction
If chlorophyll is green, what color does it absorb? Correct
Answer It absorbs every pigment except for green, which it
reflects.
If the cell cycle is 1000 minutes, and 30% of the time is spent in
mitosis, how long is interphase? Correct Answer 700 minutes
In microscopy, what is depth of field? Correct Answer The
thickness of the specimen that may be seen in focus at one time
(the vertical range of focus)
COMPLETE SOLUTIONS
A longer wavelength is associated with a lower or higher
resolution? Correct Answer Lower resolution
A scientifically useful hypothesis must be...? Correct Answer
testable and falsifiable (able to be proved false)
After separating the pigments, what was your objective?
Correct Answer Measure the absorbency of light
Amylase breaks starch down to what sugar? Correct Answer
Maltose
Anaphase Correct Answer Sister chromatids are separated and
move to opposite ends of the cell
Benedict's Reagent color changes from blue to yellow in the
presence of glucose. What chemical rxn causes this color
change? Correct Answer It's caused by the reduction of
copper
Cytokinesis Correct Answer Division of the cytoplasm
forming two daughter cells
Even though the hypothesis can be falsified, it can never be
proven true. The evidence from an investigation can only...?
Correct Answer provide support for the hypothesis
,For a question to be pursued by scientists, the phenomenon must
be _____? The elements must be _____? Correct Answer 1.
well defined and testable
2. measurable and controllable
How can you test a hypothesis? Correct Answer Experiments,
additional observations, synthesis of information available
How could you tell if amylase had a low activity level? Correct
Answer There was no change in color over a long period of
time.
How could you test to see if amylase was permanently denatured
at a high temperature? Correct Answer Lower the temperature
to an optimum level and see if the time of change or a change in
color occurs. If there is no change, amylase has been irreversibly
inactivated (denatured).
How did you know that starch did not diffuse out of the bag?
Correct Answer If starch had diffused out of the bag, it would
have reacted with the potassium iodide solution in the beaker
and turned it purple. We knew it did't because there was no color
change. We can assume starch was unable to permeate through
the bag.
How do we know if the sample of Benedict's Reagent actually
worked? Correct Answer We had a positive control in the
BAG SAMPLE. The bag sample contained a heavy
concentration of glucose and was expected to have changed
orange/red in color. The beaker solution also contained glucose,
be it in less concentration.
, How do we know when the enzyme was active? Correct
Answer "If there was slow change, the was low activity. If
there was fast change, there was high activity."
How do you calculate the retardation factor? Correct Answer
Migration distance of the substance / migration distance of the
solvent front
How do you calculate total magnification? Correct Answer
Eyepiece /optical lens x objective lens
How does adding oil between the specimen and objective lens
increase resolution? What do you change in the equation?
Correct Answer The oil increases dispersion of light, what is
changed is the refractive index.
If _____, then _____. Correct Answer 1. hypothesis
2. prediction
If chlorophyll is green, what color does it absorb? Correct
Answer It absorbs every pigment except for green, which it
reflects.
If the cell cycle is 1000 minutes, and 30% of the time is spent in
mitosis, how long is interphase? Correct Answer 700 minutes
In microscopy, what is depth of field? Correct Answer The
thickness of the specimen that may be seen in focus at one time
(the vertical range of focus)
