CRCST Exam Prep: Complete HSPA Content Outline
Guide
Responsibility is on the employer: Written ECP (exposure
control plan) summarizes protection of workers from
exposure to blood- borne diseases.
-determination of employee exposure
-implementation of exposure contgrol
-universal (standard) precautions
-engineering and work practice controls to remove hazard
-use of PPE
-housekeeping
-hep B vaccine
-post-exposure eval and follow up
1. OSHA -communications of hazards to employees
Bloodborne -training regarding hazards
pathogen -recordkeeping
standard -use of biohazard labels and sharps containers
2. pathogen microorganisms that can cause illness
3. micron 1/25,000 of an inch or 1/1,000 of a millimeter
4. aerobic requiring the presence of air or free oxygen
5. anaerobic bacteria that can live in the absence of atmospheric oxygen
6. coccus bacteria shaped like a circle or sphere (spherical)
7. bacillus bacteria shaped like rods or bricks (rod)
8. spirilla bacteria shaped like spirals (spiral)
9. dangers of endospores resistant to disinfection and other conditions such as heat,
(a different form of mak- ing them very diflcult to kill
bacteria that developed
a thick coat
1/
46
, CRCST Exam Prep: Complete HSPA Content Outline
Guide
around the cell's
nucleus when
conditions required
for growth are not
ade- quate)
10. gram staining multi-step process used to identify shapes and characteristic
of bacteria (specimen is aflxed to a slide that is first
stained purple, with crystal violet. Then stained with iodine,
discolored using alcohol or acetone, and finally stained
with safranin.
11. gram-negative bacteria that have an outer membrane that won't retain the
pur-
ple stain after treatment with iodine; they will stain pink (ex:
e.coli and salmonella)
12. gram-positive bacteria that have no outer membrane and will retain the
purple
stain, even if a decolorizer is used (ex: staph and c-ditt)
13. acid-fast (ziehl-neilson stain) some acid-fast bacilli are rod-shaped and are very
diflcult to
stain, but once stained and heat or other agents are
used, the bacteria will resist decolorization with a diute
acid-alcohol solu- tion (ex: TB and leprosy)
14. pH measure of alkalinity or acidity on a scale of 0-14; pH of
7 is neutral; below 7 is acid; above 7 is alkaline
15. psychrophile bacteria whose optimum temperature for growth is 59°-68°
16. mesophile bacteria that grow best at moderate temps 68°-113°
17. thermophile bacteria that grow best at temps of 122°-158°
2/
46
, CRCST Exam Prep: Complete HSPA Content Outline
Guide
18. vegetative state active growth of microorganisms (as opposed to resting or
spore
stages)
3/
46
, CRCST Exam Prep: Complete HSPA Content Outline
Guide
19. angstrom a unit used to measure length of light waves
20. virus the smallest microorganisms and about 1,000 times
smaller than bacteria
21. protozoa one-celled animal organisms that vary widely in size and
shape and contain no cell walls
22. fungi plant-like organisms that include molds, mushrooms, and
yeasts
23. prions an infectious protein particle that, unlike a virus,
contains no nucleic acid, does not trigger an immune
response, and is not destroyed by extreme heat or
cold
24. prion diseases or TSEs rare progressive neurodegenerative disorders that
(transmissible attect hu- mans and animals. Rapidly progressive and
spongiform always fatal. Ex: CJD (Creutzfeldt-Jakob Disease) - only
encephalopathies) about 200 cases per year in U.S
25. proper instrument cleaning, How does CS support infection prevention?
disinfection, and
steriliza- tion
26. cross-contamination migration of contaminants from one person, object, or work
location to another
27. chain of infection six steps; causative agent, reservoir of the agent, portal of
exit,
mode of transmission, portal of entry, susceptible host
28. safety data sheet - what is it? A written statement providing detailed information
about a
and where do you find it?chemical or toxic substance, including potential hazards and ap-
4/
46
Guide
Responsibility is on the employer: Written ECP (exposure
control plan) summarizes protection of workers from
exposure to blood- borne diseases.
-determination of employee exposure
-implementation of exposure contgrol
-universal (standard) precautions
-engineering and work practice controls to remove hazard
-use of PPE
-housekeeping
-hep B vaccine
-post-exposure eval and follow up
1. OSHA -communications of hazards to employees
Bloodborne -training regarding hazards
pathogen -recordkeeping
standard -use of biohazard labels and sharps containers
2. pathogen microorganisms that can cause illness
3. micron 1/25,000 of an inch or 1/1,000 of a millimeter
4. aerobic requiring the presence of air or free oxygen
5. anaerobic bacteria that can live in the absence of atmospheric oxygen
6. coccus bacteria shaped like a circle or sphere (spherical)
7. bacillus bacteria shaped like rods or bricks (rod)
8. spirilla bacteria shaped like spirals (spiral)
9. dangers of endospores resistant to disinfection and other conditions such as heat,
(a different form of mak- ing them very diflcult to kill
bacteria that developed
a thick coat
1/
46
, CRCST Exam Prep: Complete HSPA Content Outline
Guide
around the cell's
nucleus when
conditions required
for growth are not
ade- quate)
10. gram staining multi-step process used to identify shapes and characteristic
of bacteria (specimen is aflxed to a slide that is first
stained purple, with crystal violet. Then stained with iodine,
discolored using alcohol or acetone, and finally stained
with safranin.
11. gram-negative bacteria that have an outer membrane that won't retain the
pur-
ple stain after treatment with iodine; they will stain pink (ex:
e.coli and salmonella)
12. gram-positive bacteria that have no outer membrane and will retain the
purple
stain, even if a decolorizer is used (ex: staph and c-ditt)
13. acid-fast (ziehl-neilson stain) some acid-fast bacilli are rod-shaped and are very
diflcult to
stain, but once stained and heat or other agents are
used, the bacteria will resist decolorization with a diute
acid-alcohol solu- tion (ex: TB and leprosy)
14. pH measure of alkalinity or acidity on a scale of 0-14; pH of
7 is neutral; below 7 is acid; above 7 is alkaline
15. psychrophile bacteria whose optimum temperature for growth is 59°-68°
16. mesophile bacteria that grow best at moderate temps 68°-113°
17. thermophile bacteria that grow best at temps of 122°-158°
2/
46
, CRCST Exam Prep: Complete HSPA Content Outline
Guide
18. vegetative state active growth of microorganisms (as opposed to resting or
spore
stages)
3/
46
, CRCST Exam Prep: Complete HSPA Content Outline
Guide
19. angstrom a unit used to measure length of light waves
20. virus the smallest microorganisms and about 1,000 times
smaller than bacteria
21. protozoa one-celled animal organisms that vary widely in size and
shape and contain no cell walls
22. fungi plant-like organisms that include molds, mushrooms, and
yeasts
23. prions an infectious protein particle that, unlike a virus,
contains no nucleic acid, does not trigger an immune
response, and is not destroyed by extreme heat or
cold
24. prion diseases or TSEs rare progressive neurodegenerative disorders that
(transmissible attect hu- mans and animals. Rapidly progressive and
spongiform always fatal. Ex: CJD (Creutzfeldt-Jakob Disease) - only
encephalopathies) about 200 cases per year in U.S
25. proper instrument cleaning, How does CS support infection prevention?
disinfection, and
steriliza- tion
26. cross-contamination migration of contaminants from one person, object, or work
location to another
27. chain of infection six steps; causative agent, reservoir of the agent, portal of
exit,
mode of transmission, portal of entry, susceptible host
28. safety data sheet - what is it? A written statement providing detailed information
about a
and where do you find it?chemical or toxic substance, including potential hazards and ap-
4/
46
