Cytogenetics ASCP TEST
Day 1 FISH - CORRECT ANSWER-1. Denatured in 2xSSC for 2 mins
2. EtOH bath for 2 mins each (70%,80%,90%)
3. Dry very well
ALL FISH B-CELL - CORRECT ANSWER--CEP 4,10,17
-t(9;22) BCR/ABL DUAL COLOR/DUAL FUSION
-t(12;21) ETV6/RUNX1 DUAL COLOR SINGLE FUSION
-11q23 MLL (rearrangement/deletion break apart)
ALL FISH T-CELL - CORRECT ANSWER--t(9;22) BCR/ABL
AML FISH - CORRECT ANSWER--t(8;21)
-INV(16) OR t(16;16)
-11q23 MLL rearrangement/deletion
45, X - CORRECT ANSWER-Turner syndrome, webbing of the neck, short stature,
amnorrhea
47, XXY - CORRECT ANSWER-Klinefelter syndrome, tall stature, sterility,
gynecomastia
47, XX, +13 - CORRECT ANSWER-Patau syndrome, severe mental retardation, and
physical deformities
47, XX,+18 - CORRECT ANSWER-Edwards syndrome
del(4)(p16) - CORRECT ANSWER-Wolf Hirschhorn
del(5)(p15) - CORRECT ANSWER-Cri-du-Chat
del(13)(q14) - CORRECT ANSWER-retinoblastoma
dup(22)(pterq-11) - CORRECT ANSWER-cat's eye syndrome
TEL/AML - CORRECT ANSWER--t(12;21)
-ETV6-12
-RUNX1-21
M2 - CORRECT ANSWER--t(8;21)(q22;q22)
ETO/RUNx1
-RUNx1T1-8q21.3
-RUNx1-21q22
Day 1 FISH - CORRECT ANSWER-1. Denatured in 2xSSC for 2 mins
2. EtOH bath for 2 mins each (70%,80%,90%)
3. Dry very well
ALL FISH B-CELL - CORRECT ANSWER--CEP 4,10,17
-t(9;22) BCR/ABL DUAL COLOR/DUAL FUSION
-t(12;21) ETV6/RUNX1 DUAL COLOR SINGLE FUSION
-11q23 MLL (rearrangement/deletion break apart)
ALL FISH T-CELL - CORRECT ANSWER--t(9;22) BCR/ABL
AML FISH - CORRECT ANSWER--t(8;21)
-INV(16) OR t(16;16)
-11q23 MLL rearrangement/deletion
45, X - CORRECT ANSWER-Turner syndrome, webbing of the neck, short stature,
amnorrhea
47, XXY - CORRECT ANSWER-Klinefelter syndrome, tall stature, sterility,
gynecomastia
47, XX, +13 - CORRECT ANSWER-Patau syndrome, severe mental retardation, and
physical deformities
47, XX,+18 - CORRECT ANSWER-Edwards syndrome
del(4)(p16) - CORRECT ANSWER-Wolf Hirschhorn
del(5)(p15) - CORRECT ANSWER-Cri-du-Chat
del(13)(q14) - CORRECT ANSWER-retinoblastoma
dup(22)(pterq-11) - CORRECT ANSWER-cat's eye syndrome
TEL/AML - CORRECT ANSWER--t(12;21)
-ETV6-12
-RUNX1-21
M2 - CORRECT ANSWER--t(8;21)(q22;q22)
ETO/RUNx1
-RUNx1T1-8q21.3
-RUNx1-21q22
