Which of the following is true about the difference between nucleotides and nucleosides?
•A. Nucleotides lack a nitrogenous base.
•B. Nucleosides lack a ribose sugar.
•C. Nucleotides lack a phosphate.
•D. Nucleosides lack a phosphate. - Answers D
The chemical synthesis of DNA
•A. occurs in a 5′ to 3′ direction.
•B. is carried out with the growing strand attached to a solid support.
•C. can add one or more nucleotides per chemical cycle.
D. requires a DNA template. - Answers B
Deoxyribonucleic acid (DNA) is different from ribonucleic acid (RNA) in that
•A. DNA contains a 3′ H, whereas RNA contains a 3′ OH.
•B. only DNA utilizes the base uracil.
•C. only RNA can be hydrolyzed by alkaline conditions.
D. only DNA contains a pentose sugar. - Answers C
Evidence that DNA stores the genetic information of the cell was provided by Avery-MacLeod-
McCarty experiment in which
•A. DNA from a heat-killed virulent strain of the bacterium Streptococcus pneumoniae could
genetically transform live non virulent strain into a virulent strain.
•B. DNA from a heat-killed nonvirulent strain of the bacterium Streptococcus pneumoniae could
transform live non virulent strain into a virulent strain.
•C. a live nonvirulent strain of the bacterium Streptococcus pneumoniae could be transformed
into a virulent strain by heat treatment.
•D. DNA from a heat-killed virulent strain of the bacterium Streptococcus pneumoniae could
transform a live nonvirulent strain if the DNA was treated with deoxyribonucleases. - Answers A
, Which of the following is true about the denaturation of double-helical DNA?
•A. Denaturation increases with decreasing temperature.
•B. Denaturation is accompanied by an increase in the absorption of UV light by DNA.
•C. G-C rich DNA melts at lower temperature than A-T rich DNA.
•D. Once denatured, DNA strands cannot anneal. - Answers B
Which of the following is true about the structure of DNA as proposed by Watson and Crick (B-
form DNA)?
•A. DNA forms a left-handed double helix.
•B. The strands of DNA are parallel.
•C. The complementary base pairs are held together by hydrogen bonds.
•D. A complete turn of the double helix occurs after every base pair. - Answers C
"Chargaff's rules" about the composition of bases in DNA dictates that
•A. the sum of purine residues must equal the sum of pyrimidine residues.
•B. the sum of A-T base pairs must equal the sum of G-C base pairs.
•C. the base composition of DNA is the same in all species.
•D. DNA specimens isolated from different tissues of the same species vary in base
composition. - Answers A
DNA can be sequenced by the Sanger method in which
•A. radioactively labeled DNA is subjected to four sets of base-specific cleavage reactions.
•B. automated sequencing can be done using different fluorescent-labeled 2′ deoxynucleotides.
•C. the required DNA synthesis is performed via automated chemical synthesis.
•D. the sequence can be read by a laser beam. - Answers D
Which of the following is true about DNA sequence palindromes?
•A. A palindrome can form a cruciform structure involving one strand of DNA.
•B. Palindromes have two-fold symmetry over two strands of DNA.
•C. Palindromes are also known as mirror repeats.