8/10/25, 5:56 AM CHT EXAM COMPLETE STUDY GUIDE 2024 Flashcards | Quizlet
CHT EXAM COMPLETE STUDY Save
GUIDE 2024
Terms in this set (896)
It is possible to convert Calcium is added to the plasma to form a complex
the plasma with the citrate present in the anti-coagulant. This
into serum by recalcifying restores a normal
the plasma and allowing ionized calcium level allowing clotting to occur.
coagulation to occur.
Polyspecific antiserum absorbed
can be______ with
lymphocytes to determine
if there is a single antibody
or multiple
antibodies.
Another application of autoantibody
lymphocyte
absorption is to use the
patient's own cells to
remove_____. Absorption
can be performed
at cold or warm
temperatures depending
on the type of antibody.
_________ are typically Autoantibodies
cold reacting (4oC) B cell antibodies
and________are typically
warm reacting (22-37oC).
https://quizlet.com/1064849449/cht-exam-complete-study-guide-2024-flash-cards/?new 1/18
,8/10/25, 5:56 AM CHT EXAM COMPLETE STUDY GUIDE 2024 Flashcards | Quizlet
Patient serum may contain false positive reactivity.
autoantibodies and/or
IgM antibodies that may
interfere the binding of
anti-HLA IgG antibodies to
the antigen. In
complement dependent
assays (CDC), IgM
antibodies
can activate complement;
potentially leading to
___________.
DTT dithiothreitol, also known as Cleland's Reagent
DTT inactivates IgM disulphide bonds
molecules by cleaving the
_________ in the
pentamer structure.
Heat inactivation works by DTT.
cleaving the disulphide
bonds in the
pentamer structure, but is
less specific than _____.
therapeutic antilymphocyte
ALA
antibodies
purified, pasteurized, gamma immune globulin,
Thymoglobulin obtained by immunization of rabbits with human
thymocytes
a murine IgG monoclonal antibody directed against
OKT3
CD3
https://quizlet.com/1064849449/cht-exam-complete-study-guide-2024-flash-cards/?new 2/18
, 8/10/25, 5:56 AM CHT EXAM COMPLETE STUDY GUIDE 2024 Flashcards | Quizlet
If these therapeutic false positive
antilymphocyte
antibodies are present in
the patient serum, they
can cause_______ reactivity
in
T cell cytotoxic assays.
ALA removal is magnetic beads
accomplished by
absorption using
_____coated with anti-rabbit
or anti-mouse
immunoglobulin for
Thymoblobulin or OKT3,
respectively.
1. Recalcificiation of plasma
2. Adsorption with lymphocytes
3. Inactivation of IgM antibodies
serum preparations a. DTT treatment
b. Heat Inactivation
4. Depletion of therapeutic anti-lymphocyte
antibodies from serum
Calcium is added to the plasma to combine with the
Why is calcium added to
citrate present in the anticoagulant.
plasma to convert it to
This restores a normal ionized calcium level and
serum?
allows clotting to occur.
At what temperature Typically, auto-antibodies are cold reacting and
should serum absorption should be absorbed at 4oC while alloantibodies
with lymphocytes be are warm reacting and should be absorbed at 37oC.
performed?
https://quizlet.com/1064849449/cht-exam-complete-study-guide-2024-flash-cards/?new 3/18
CHT EXAM COMPLETE STUDY Save
GUIDE 2024
Terms in this set (896)
It is possible to convert Calcium is added to the plasma to form a complex
the plasma with the citrate present in the anti-coagulant. This
into serum by recalcifying restores a normal
the plasma and allowing ionized calcium level allowing clotting to occur.
coagulation to occur.
Polyspecific antiserum absorbed
can be______ with
lymphocytes to determine
if there is a single antibody
or multiple
antibodies.
Another application of autoantibody
lymphocyte
absorption is to use the
patient's own cells to
remove_____. Absorption
can be performed
at cold or warm
temperatures depending
on the type of antibody.
_________ are typically Autoantibodies
cold reacting (4oC) B cell antibodies
and________are typically
warm reacting (22-37oC).
https://quizlet.com/1064849449/cht-exam-complete-study-guide-2024-flash-cards/?new 1/18
,8/10/25, 5:56 AM CHT EXAM COMPLETE STUDY GUIDE 2024 Flashcards | Quizlet
Patient serum may contain false positive reactivity.
autoantibodies and/or
IgM antibodies that may
interfere the binding of
anti-HLA IgG antibodies to
the antigen. In
complement dependent
assays (CDC), IgM
antibodies
can activate complement;
potentially leading to
___________.
DTT dithiothreitol, also known as Cleland's Reagent
DTT inactivates IgM disulphide bonds
molecules by cleaving the
_________ in the
pentamer structure.
Heat inactivation works by DTT.
cleaving the disulphide
bonds in the
pentamer structure, but is
less specific than _____.
therapeutic antilymphocyte
ALA
antibodies
purified, pasteurized, gamma immune globulin,
Thymoglobulin obtained by immunization of rabbits with human
thymocytes
a murine IgG monoclonal antibody directed against
OKT3
CD3
https://quizlet.com/1064849449/cht-exam-complete-study-guide-2024-flash-cards/?new 2/18
, 8/10/25, 5:56 AM CHT EXAM COMPLETE STUDY GUIDE 2024 Flashcards | Quizlet
If these therapeutic false positive
antilymphocyte
antibodies are present in
the patient serum, they
can cause_______ reactivity
in
T cell cytotoxic assays.
ALA removal is magnetic beads
accomplished by
absorption using
_____coated with anti-rabbit
or anti-mouse
immunoglobulin for
Thymoblobulin or OKT3,
respectively.
1. Recalcificiation of plasma
2. Adsorption with lymphocytes
3. Inactivation of IgM antibodies
serum preparations a. DTT treatment
b. Heat Inactivation
4. Depletion of therapeutic anti-lymphocyte
antibodies from serum
Calcium is added to the plasma to combine with the
Why is calcium added to
citrate present in the anticoagulant.
plasma to convert it to
This restores a normal ionized calcium level and
serum?
allows clotting to occur.
At what temperature Typically, auto-antibodies are cold reacting and
should serum absorption should be absorbed at 4oC while alloantibodies
with lymphocytes be are warm reacting and should be absorbed at 37oC.
performed?
https://quizlet.com/1064849449/cht-exam-complete-study-guide-2024-flash-cards/?new 3/18
