“Lab 2 Culturing & Aseptic Technique BIO250L”
Student Name: Click here to enter text.
Access Code (located on the lid of your lab kit): Click here to enter text.
Lab Report Format Expectations
Utilize college level grammar and professional formatting when completing this worksheet.
Submissions without proper formatting, all required photos or sufficient responses will be rejected.
Pre-lab Questions
1. This lab includes two experiments that each look at different aspects of culturing
microorganisms onto growth media. What concepts does each experiment focus on? Why do
you think these concepts are important to the field of microbiology?
Culturing and aseptic technique in microbiology focus on fundamental concepts related to the
cultivation, maintenance and manipulation of microorganisms in laboratory settings. These
concepts are are essential for research, diagnosis and biotechnological processes.
2. In this lab, the experiments will allow or call for the use of four inoculation tools. List these tools
and provide a scenario in which you would use each tool.
○ Innoculation Loop- to pick single colony and to spread on the meidum to get isolated
colonies.
○ Inoculation Needle- used for stab inoculation of semi-solid media or for making single
point inoculations
○ Pipettes- used for transfering liquid cultures, making dilutions or adding specific volumes
of liquids to media.
○ Bacterial Spreaders- used for spreading bacterial suspensions evenly over the surface of
soild media.
3. Describe a piece of equipment used in biotech labs to extend the growth pattern of microbes.
Focus particularly on those used with E. coli used to make human insulin.
A piece of equipment used in biotech labs to extend the growth pattern of microbes would be a
bioreactor.
4. You’re a physician trying to isolate bacterial colonies from the human gut in an attempt to
diagnose a gastrointestinal infection. You streak your sample on a growth media containing
glucose, amino acids, and salts containing both sulfur and phosphorus with a pH of 7. You
incubate the plates in aerobic conditions at 37 ˚C for three days, at which point you can see
clear bacterial colonies forming on the plate. Would you feel confident in stating that you had
, “Lab 2 Culturing & Aseptic Technique BIO250L”
successfully cultured all the bacteria from your gut sample? Why or why not?
I am asure that I cultured the bacteria from the gut sample,because there was bacterial growth
growing.
EXPERIMENT 1: AGAR PLATE PREPARATION AND BACTERIAL INOCULATION
Introduction Questions
1. Describe the objectives of Experiment 1. State the goal of the experiment and the learning
outcome that it is trying to convey to you as a microbiology student.
The goal of bacterial inoculation and agar plate preparation in microbiology .As a microbiology
student, performing bacterial inoculation and agar preparation serves several education
objectives like isolation of pure cultures, identification and characterization.
2. Proper aseptic technique is crucial to ensuring the growth of pure bacterial colonies. What will
you do in this lab to demonstrate that you are using proper aseptic technique?
Proper aseptic techniques are as follows, hand washing, flaming the inoculation loop or needles,
avoiding splattering, closing tubed or petri dishes quickly.
In a laboratory setting, what are three ways you can properly sterilize culturing equipment?
○ Autoclaving
○ Dry heat Sterilization
○ chemical sterilization
3. What method of sterilization will you use in this experiment?
nutrient agar
4. What results do you expect to see with this experiment? For example, do you expect to see
growth from all surfaces or just some? Are there particular strains you expect from the surfaces
you swabbed?
Staphylococcus, small round colonies. .
Student Name: Click here to enter text.
Access Code (located on the lid of your lab kit): Click here to enter text.
Lab Report Format Expectations
Utilize college level grammar and professional formatting when completing this worksheet.
Submissions without proper formatting, all required photos or sufficient responses will be rejected.
Pre-lab Questions
1. This lab includes two experiments that each look at different aspects of culturing
microorganisms onto growth media. What concepts does each experiment focus on? Why do
you think these concepts are important to the field of microbiology?
Culturing and aseptic technique in microbiology focus on fundamental concepts related to the
cultivation, maintenance and manipulation of microorganisms in laboratory settings. These
concepts are are essential for research, diagnosis and biotechnological processes.
2. In this lab, the experiments will allow or call for the use of four inoculation tools. List these tools
and provide a scenario in which you would use each tool.
○ Innoculation Loop- to pick single colony and to spread on the meidum to get isolated
colonies.
○ Inoculation Needle- used for stab inoculation of semi-solid media or for making single
point inoculations
○ Pipettes- used for transfering liquid cultures, making dilutions or adding specific volumes
of liquids to media.
○ Bacterial Spreaders- used for spreading bacterial suspensions evenly over the surface of
soild media.
3. Describe a piece of equipment used in biotech labs to extend the growth pattern of microbes.
Focus particularly on those used with E. coli used to make human insulin.
A piece of equipment used in biotech labs to extend the growth pattern of microbes would be a
bioreactor.
4. You’re a physician trying to isolate bacterial colonies from the human gut in an attempt to
diagnose a gastrointestinal infection. You streak your sample on a growth media containing
glucose, amino acids, and salts containing both sulfur and phosphorus with a pH of 7. You
incubate the plates in aerobic conditions at 37 ˚C for three days, at which point you can see
clear bacterial colonies forming on the plate. Would you feel confident in stating that you had
, “Lab 2 Culturing & Aseptic Technique BIO250L”
successfully cultured all the bacteria from your gut sample? Why or why not?
I am asure that I cultured the bacteria from the gut sample,because there was bacterial growth
growing.
EXPERIMENT 1: AGAR PLATE PREPARATION AND BACTERIAL INOCULATION
Introduction Questions
1. Describe the objectives of Experiment 1. State the goal of the experiment and the learning
outcome that it is trying to convey to you as a microbiology student.
The goal of bacterial inoculation and agar plate preparation in microbiology .As a microbiology
student, performing bacterial inoculation and agar preparation serves several education
objectives like isolation of pure cultures, identification and characterization.
2. Proper aseptic technique is crucial to ensuring the growth of pure bacterial colonies. What will
you do in this lab to demonstrate that you are using proper aseptic technique?
Proper aseptic techniques are as follows, hand washing, flaming the inoculation loop or needles,
avoiding splattering, closing tubed or petri dishes quickly.
In a laboratory setting, what are three ways you can properly sterilize culturing equipment?
○ Autoclaving
○ Dry heat Sterilization
○ chemical sterilization
3. What method of sterilization will you use in this experiment?
nutrient agar
4. What results do you expect to see with this experiment? For example, do you expect to see
growth from all surfaces or just some? Are there particular strains you expect from the surfaces
you swabbed?
Staphylococcus, small round colonies. .
