BIOD 171: MODULE 4 REal
Study online at https://quizlet.com/_hlz9hu
False
T/F: Growth media is best suited for dis-
tinguishing between two similar species
growth media is designed to simply sup-
of bacteria
port (and not restrict) microbial growth
A researcher is asked to determine
which of two vials contains E coli
and which contains salmonella. Knowing
both are Gram-negative while only one Differential media
of them is capable of fermenting lactose,
which type of media would be best suit-
ed?
A fastidious microbe is an organism with
complex growth requirements and will
What are the requirements of a fastidi- not grow without then. Enriched medias
ous microbe? thus contain these specific and essential
nutrients required for the growth of a par-
ticular subset of microorganisms.
T/F: LB agar is classified as a non-selec-
True
tive, non-differential media
The polysaccharide-based hardening
agent added to LB media to produce LB Seaweed extract
agar is derived from what?
Blood agar is which type of medium?
Select all that apply:
- Selective and Differential - Enriched
- Enriched - Differential
- Differential
- Selective
Match the following hemolytic class with
its description of activity:
1. Alpha hemolysis 1. Alpha hemolysis- Greenish-brown col-
2. Beta hemolysis or (B)
3. Gamma hemolysis 2. Beta hemolysis- Distinct zone of clear-
ing (C)
A. No change 3. Gamma hemolysis- No change (A)
B. Greenish-brown color
C. Distinct zone of clearing
1/6
, BIOD 171: MODULE 4 REal
Study online at https://quizlet.com/_hlz9hu
Match the following hemolytic class with
its description of activity:
1. Alpha hemolysis- Incomplete hemolyt-
1. Alpha hemolysis ic activity (B)
2. Beta hemolysis 2. Beta hemolysis- Complete hemolytic
3. Gamma hemolysis activity (C)
3. Gamma hemolysis- No hemolytic ac-
A. No hemolytic activity tivity (A)
B. Incomplete hemolytic activity
C. Complete hemolytic activity
Blood agar
Columbia CNA agar is most closely re-
CNA agar is similar to BAP as its also
lated to which media:
enriched with blood and allows for differ-
entiation based on hemolytic patterns
False
T/F: Chocolate agar gets its brown color chocolate is never added to the media.
from cocoa to produce an enriched me- the name is derived simply based on the
dia color that actually comes from the pres-
ence of 'cooked' (lysed) red blood cells in
the media
A researcher is studying a strain of E. coli
currently growing on a MacConkey plate.
However, the researcher can't remember No, because gram negative microbes
if E. coli is Gram-positive or Gram-nega- only grow on macConkey agar
tive. Would a Gram stain be necessary to
confirm? Why or why not?
In an attempt to detect the presence of
The SMAC should be tried next because
the pathogenic strain of E. coli O157:H7,
it can be differentiated by lactose fermen-
a researcher spread a culture onto
tation and sorbitol fermentation, both of
a MacConkey agar with failed results.
which can be fermented by the non-path-
What type of agar should they (correctly)
ogenic strain
try next? Why?
What is the Gram status (positive or Gram-negative because gram positive
negative) of microbes growing on Eosin bacteria growth is restricted by EMB
Methylene Blue (EMB) agar plates? plates
2/6
Study online at https://quizlet.com/_hlz9hu
False
T/F: Growth media is best suited for dis-
tinguishing between two similar species
growth media is designed to simply sup-
of bacteria
port (and not restrict) microbial growth
A researcher is asked to determine
which of two vials contains E coli
and which contains salmonella. Knowing
both are Gram-negative while only one Differential media
of them is capable of fermenting lactose,
which type of media would be best suit-
ed?
A fastidious microbe is an organism with
complex growth requirements and will
What are the requirements of a fastidi- not grow without then. Enriched medias
ous microbe? thus contain these specific and essential
nutrients required for the growth of a par-
ticular subset of microorganisms.
T/F: LB agar is classified as a non-selec-
True
tive, non-differential media
The polysaccharide-based hardening
agent added to LB media to produce LB Seaweed extract
agar is derived from what?
Blood agar is which type of medium?
Select all that apply:
- Selective and Differential - Enriched
- Enriched - Differential
- Differential
- Selective
Match the following hemolytic class with
its description of activity:
1. Alpha hemolysis 1. Alpha hemolysis- Greenish-brown col-
2. Beta hemolysis or (B)
3. Gamma hemolysis 2. Beta hemolysis- Distinct zone of clear-
ing (C)
A. No change 3. Gamma hemolysis- No change (A)
B. Greenish-brown color
C. Distinct zone of clearing
1/6
, BIOD 171: MODULE 4 REal
Study online at https://quizlet.com/_hlz9hu
Match the following hemolytic class with
its description of activity:
1. Alpha hemolysis- Incomplete hemolyt-
1. Alpha hemolysis ic activity (B)
2. Beta hemolysis 2. Beta hemolysis- Complete hemolytic
3. Gamma hemolysis activity (C)
3. Gamma hemolysis- No hemolytic ac-
A. No hemolytic activity tivity (A)
B. Incomplete hemolytic activity
C. Complete hemolytic activity
Blood agar
Columbia CNA agar is most closely re-
CNA agar is similar to BAP as its also
lated to which media:
enriched with blood and allows for differ-
entiation based on hemolytic patterns
False
T/F: Chocolate agar gets its brown color chocolate is never added to the media.
from cocoa to produce an enriched me- the name is derived simply based on the
dia color that actually comes from the pres-
ence of 'cooked' (lysed) red blood cells in
the media
A researcher is studying a strain of E. coli
currently growing on a MacConkey plate.
However, the researcher can't remember No, because gram negative microbes
if E. coli is Gram-positive or Gram-nega- only grow on macConkey agar
tive. Would a Gram stain be necessary to
confirm? Why or why not?
In an attempt to detect the presence of
The SMAC should be tried next because
the pathogenic strain of E. coli O157:H7,
it can be differentiated by lactose fermen-
a researcher spread a culture onto
tation and sorbitol fermentation, both of
a MacConkey agar with failed results.
which can be fermented by the non-path-
What type of agar should they (correctly)
ogenic strain
try next? Why?
What is the Gram status (positive or Gram-negative because gram positive
negative) of microbes growing on Eosin bacteria growth is restricted by EMB
Methylene Blue (EMB) agar plates? plates
2/6
