Enterobacteriaceae Flashcards

Enterobacteriaceae Flashcards mm




Biochemically, the Enterobacteriaceae are gram-negative rods that:
mm mm mm mm mm mm




A. Ferment glucose, reduce nitrate to nitrite, and are oxidase negative
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B. Ferment glucose, produce indophenol oxidase, and form gas
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C. Ferment lactose and reduce nitrite to nitrogen gas
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D. Ferment lactose and produce indophenol oxidase - ANS>>A. Ferment glucose,
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reduce nitrate to nitrite, and
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are oxidase negative
mm mm




The ortho-nitrophenyl-β-galactopyranoside (ONPG) test is most useful when
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differentiating:
mm mm




A. Salmonella spp. from Pseudomonas spp.
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B. Shigella spp. from some strains of Escherichia coli
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C. Klebsiella spp. from Enterobacter spp.
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D. Proteus vulgaris from Salmonella spp. - ANS>>B. Shigella spp. from some
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strains of Escherichia coli
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The Voges-Proskauer (VP) test detects which end product of glucose
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fermentation?
mm




A. mm Acetoin
B. mm Nitrite
C. mm Acetic acid mm



D. mm Hydrogen sulfide - ANS>>A. Acetoin mm mm mm mm




At which pH does the methyl red (MR) test become positive?
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A. mm 7.0
B. mm 6.5
C. mm 6.0 mm



D. mm 4.5 - ANS>>D. 4.5
mm mm mm




A positive Simmons citrate test is seen as a:
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A. Blue color in the medium after 24 hours of incubation at 35°C
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B. Red color in the medium after 18 hours of incubation at 35°C
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C. Yellow color in the medium after 24 hours of incubation at 35°C
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, D. Green color in the medium after 18 hours of incubation at 35°C - ANS>>A.
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Blue color in the medium after 24 hours of incubation at 35°C
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In the test for urease production, ammonia reacts to form which product?
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A. mm Ammonium mm citrate
B. mm Ammonium mm carbonate
C. mm Ammonium mm oxalate mm



D. mm Ammonium mm nitrate - ANS>>B. Ammonium carbonate
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Which of the following reagents is added to detect the production of indole?
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A. mm p-Dimethylaminobenzaldehyde
B. mm Bromcresol purple mm



C. mm Methyl red mm



D. mm Cytochrome oxidase - ANS>>A. p-Dimethylaminobenzaldehyde
mm mm mm mm




Decarboxylation of the amino acids lysine, ornithine, and arginine results in the
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formation of:
mm mm




A. mm Ammonia
B. mm Urea mm



C. mm CO2
D. mm Amines - ANS>>D. Aminesmm mm mm




Lysine iron agar (LIA) showing a purple slant and a blackened butt indicates:
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A. mm E. coli
mm



B. mm Citrobacter spp. mm



C. mm Salmonella spp. mm mm



D. mm Proteus spp. - ANS>>C. Salmonella spp.
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Putrescine is an alkaline amine product of which bacterial enzyme?
mm mm mm mm mm mm mm mm mm




A. mm Arginine decarboxylase mm



B. mm Phenylalanine deaminase mm



C. mm Ornithine decarboxylase mm mm



D. mm Lysine decarboxylase - ANS>>C. Ornithine decarboxylase
mm mm mm mm mm




Which genera are positive for phenylalanine deaminase?
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A. Enterobacter, Escherichia, and Salmonella
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B. Morganella, Providencia, and Proteus
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C. Klebsiella and Enterobacter
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D. Proteus, Escherichia, and Shigella - ANS>>B. Morganella, Providencia, and
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Proteus
mm