MCB 252 FINAL EXAM RESULTS
features of polyclonal antibodies - answers✔✔Many different antibodies
Diversity of use cases
Recognize distinct epitope on antigen surface
Protein purification requirement - answers✔✔Need assay that is specific for the particular
protein
Determining protein structure by X ray crystallography - answers✔✔Need purification
Amino acid sequence
Biochemistry - answers✔✔purify, study in vitro
Genetics - answers✔✔isolate mutant organism that is defective in process
Cell biology - answers✔✔follow dynamics in living cell
Advantage of GFP over immunofluorescence - answers✔✔Allows visualization of living cells and
organisms
,Transgenics - answers✔✔integrated into chromosome and maintained in all cells of living
organism
Transformation - answers✔✔DNA introduced and stably maintained
Transfection - answers✔✔DNA introduced and transiently maintained
3 steps of Nucleation of actin assembly in vitro - answers✔✔Tri molecular reaction with actin
monomer addition
Nucleation
Elongation
Steady state
Actin filaments features - answers✔✔Grow or shrink by addition or loss of monomers from the
ends
Actin, microtubules, and intermediate filaments are all multi-stranded
Which of the following is/are a property(ies) of a reaction that is at steady state?
At least one parameter of the reaction can vary over time. Incorrect At least one parameter of
the reaction remains constant over time.
At steady state the reaction requires a continuous input of energy.
At least one parameter of the reaction remains constant over time, and at least one parameter
of the reaction can vary over time.
: All of the above - answers✔✔: All of the above
, In lecture we discussed an experiment that utilized a plastic bead coated with the ActA protein.
What conclusion did we come to based on the results of that experiment?
That polymerization of actin is sufficient to move a membrane
That ActA is a motor protein that is necessary for movement of Listeria
That ATP hydrolysis by actin provides the energy required to move a membrane
That Myosin V motor heads have a 72nm step size
That stress fibers are involved in moving Listeria - answers✔✔That polymerization of actin is
sufficient to move a membrane
You are watching Listeria moving around inside two cells. You inject one cell with phalloidin.
What are you most likely to expect to see happen in the injected cell?
The bacteria in the injected cell will stop moving.
: The comet tails will become longer in the injected cell than the non-injected cell.
The comet tails will become shorter in the injected cell than the non-injected cell.
The bacteria in the injected cell will move faster than in the non-injected cell.
features of polyclonal antibodies - answers✔✔Many different antibodies
Diversity of use cases
Recognize distinct epitope on antigen surface
Protein purification requirement - answers✔✔Need assay that is specific for the particular
protein
Determining protein structure by X ray crystallography - answers✔✔Need purification
Amino acid sequence
Biochemistry - answers✔✔purify, study in vitro
Genetics - answers✔✔isolate mutant organism that is defective in process
Cell biology - answers✔✔follow dynamics in living cell
Advantage of GFP over immunofluorescence - answers✔✔Allows visualization of living cells and
organisms
,Transgenics - answers✔✔integrated into chromosome and maintained in all cells of living
organism
Transformation - answers✔✔DNA introduced and stably maintained
Transfection - answers✔✔DNA introduced and transiently maintained
3 steps of Nucleation of actin assembly in vitro - answers✔✔Tri molecular reaction with actin
monomer addition
Nucleation
Elongation
Steady state
Actin filaments features - answers✔✔Grow or shrink by addition or loss of monomers from the
ends
Actin, microtubules, and intermediate filaments are all multi-stranded
Which of the following is/are a property(ies) of a reaction that is at steady state?
At least one parameter of the reaction can vary over time. Incorrect At least one parameter of
the reaction remains constant over time.
At steady state the reaction requires a continuous input of energy.
At least one parameter of the reaction remains constant over time, and at least one parameter
of the reaction can vary over time.
: All of the above - answers✔✔: All of the above
, In lecture we discussed an experiment that utilized a plastic bead coated with the ActA protein.
What conclusion did we come to based on the results of that experiment?
That polymerization of actin is sufficient to move a membrane
That ActA is a motor protein that is necessary for movement of Listeria
That ATP hydrolysis by actin provides the energy required to move a membrane
That Myosin V motor heads have a 72nm step size
That stress fibers are involved in moving Listeria - answers✔✔That polymerization of actin is
sufficient to move a membrane
You are watching Listeria moving around inside two cells. You inject one cell with phalloidin.
What are you most likely to expect to see happen in the injected cell?
The bacteria in the injected cell will stop moving.
: The comet tails will become longer in the injected cell than the non-injected cell.
The comet tails will become shorter in the injected cell than the non-injected cell.
The bacteria in the injected cell will move faster than in the non-injected cell.
